DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251562X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251563X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251565X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251566X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251564X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251568X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251560X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251567X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Passon DM., Lee M., Rackham O., Stanley WA., Sadowska A., Filipovska A., Fox AH., Bond CS. DIP original experiment: DIP-251561X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. q8wxf1-1 Paraspeckle component 1 PSPC1 PSP1 Paraspeckle protein 1 PSP1-alpha protein protein human Homo sapiens Human MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGNQGPPPMMGMNMNNRATIPGPPMGPGPAMGPEGAANMGTPMMPDNGAVHNDRFPQGPPSQMGSPMGSRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY DIP protein Q8WXF1-1 original sequence version: 104 nono_human Non-POU domain-containing octamer-binding protein NONO NRB54 54 kDa nuclear RNA- and DNA-binding protein p54(nrb) 55 kDa nuclear protein NMT55 DNA-binding p52/p100 complex, 52 kDa subunit protein protein human Homo sapiens Human MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY DIP protein Q15233 original sequence version: 156 DIP protein Q15233 original sequence version: 148 DIP protein Q15233 original sequence version: 139 DIP protein Q15233 original sequence version: 91 26BBD3828F5B9E49 pspc1-nono-9 5 11 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait Region 61-312 Region 61-312 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association pspc1-nono-10 7 11 unspecified role unspecified role prey prey Region 53-312 Region 53-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role bait bait Region 61-320 Region 61-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association pspc1-nono-11 4 11 unspecified role unspecified role prey prey Q15233:p.[Tyr267Ala;Trp271Ala] Y267A/W271A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y267A/W271A'. Wed May 02 15:24:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr267ala,trp271ala' Tue Jun 30 19:51:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr267Ala;Trp271Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction nono-pspc1-8 1 11 unspecified role unspecified role unspecified role unspecified role Region 53-312 Region 53-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 61-320 Region 61-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pspc1-pspc1-1 3 10 unspecified role unspecified role prey prey Q8WXF1-1:p.[Tyr275Ala;Trp279Ala] Y275A/W279A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y275A/W279A'. Wed May 02 19:09:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr275ala,trp279ala' Tue Jun 30 18:28:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr275Ala;Trp279Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction nono-pspc1-9 9 11 unspecified role unspecified role unspecified role unspecified role Region 53-312 Region 53-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 61-320 Region 61-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nono-pspc1-10 2 11 unspecified role unspecified role unspecified role unspecified role Region 53-312 Region 53-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 61-320 Region 61-320 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pspc1-nono-12 8 11 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role bait bait Q8WXF1-1:p.[Tyr275Ala;Trp279Ala] Y275A/W279A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y275A/W279A'. Wed May 02 16:26:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr275ala,trp279ala' Tue Jun 30 18:31:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr275Ala;Trp279Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction nono-nono-4 6 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 11 unspecified role unspecified role prey prey Q15233:p.[Tyr267Ala;Trp271Ala] Y267A/W271A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y267A/W271A'. Wed May 02 16:11:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr267ala,trp271ala' Tue Jun 30 17:03:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr267Ala;Trp271Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction