DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of ultraviolet-B perception by UVR8. Structural basis of ultraviolet-B perception by UVR8. Nature 2012 imex curation Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. DIP original experiment: DIP-249543X Nature 2012 IMEx Structural basis of ultraviolet-B perception by UVR8. Structural basis of ultraviolet-B perception by UVR8. Structural basis of ultraviolet-B perception by UVR8. Nature 2012 imex curation Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. DIP original experiment: DIP-249544X Nature 2012 IMEx Structural basis of ultraviolet-B perception by UVR8. Structural basis of ultraviolet-B perception by UVR8. Structural basis of ultraviolet-B perception by UVR8. Nature 2012 imex curation Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wu D., Hu Q., Yan Z., Chen W., Yan C., Huang X., Zhang J., Yang P., Deng H., Wang J., Deng X., Shi Y. DIP original experiment: DIP-249542X Nature 2012 IMEx Structural basis of ultraviolet-B perception by UVR8. uvr8_arath Ultraviolet-B receptor UVR8 UVR8 Protein UV-B RESISTANCE 8 RCC1 domain-containing protein UVR8 MGI19.7 At5g63860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEDMAADEVTAPPRKVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRTDRLVPERVTSTGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLGHGDLEDHLIPHKLEALSNSFISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVGNNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGIGESVDRNFPKIIEALSVDGASGQHIESSNIDPSSGKSWVSPAERYAVVPDETGLTDGSSKGNGGDISVPQTDVKRVRI DIP protein Q9FN03 original sequence version: 86 DIP protein Q9FN03 original sequence version: 66 D2EA103CCFC92E98 uvr8-1 3 4 unspecified role unspecified role unspecified role unspecified role Core domain Region 12-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction uvr8-2 1 4 unspecified role unspecified role unspecified role unspecified role Q9FN03:p.Trp285Ala W285A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W285A'. Wed May 02 20:15:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp285ala' Tue Jun 30 20:20:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp285Ala' Core domain Region 12-381 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction uvr8-3 2 4 unspecified role unspecified role unspecified role unspecified role Core domain Region 12-381 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9FN03:p.Arg286Ala R286A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R286A'. Wed May 02 15:48:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg286ala' Tue Jun 30 18:55:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg286Ala' Q9FN03:p.Arg338Ala R338A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R338A'. Wed May 02 17:38:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg338ala' Tue Jun 30 19:57:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg338Ala' ecolx Escherichia coli direct interaction direct interaction