DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249458X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249456X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249452X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249460X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249451X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249453X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249455X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249459X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249457X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature 2012 imex curation Wiener R., Zhang X., Wang T., Wolberger C. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wiener R., Zhang X., Wang T., Wolberger C. DIP original experiment: DIP-249454X Nature 2012 IMEx The mechanism of OTUB1-mediated inhibition of ubiquitination. q13404-2 Ubiquitin-conjugating enzyme E2 variant 1 UBE2V1 CROC1 UBE2V UEV1 P/OKcl.19 CROC-1A UEV-1A CROC-1 TRAF6-regulated IKK activator 1 beta Uev1A protein protein human Homo sapiens Human MPGEVQASYLKSQSKLSDEGRLEPRKFHCKGSKSPSQFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN DIP protein Q13404-2 original sequence version: 123 otubl_caeel Ubiquitin thioesterase otubain-like C25D7.8 Deubiquitinating enzyme otubain-like otub-1 Ubiquitin-specific-processing protease otubain-like protein protein caeel Caenorhabditis elegans MANEPQKSDDNGQAAEAVVTDDEIVLQDQQLKTIEDEQKSVPLVATLAPFSILCAEYDNETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASSRDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDDQQIAPEITLLYRPGHYDVIYKKDSTEASEIEN DIP protein Q9XVR6 original sequence version: 73 262500E41F8FA23C q96fw1-1 Ubiquitin thioesterase OTUB1 OTUB1 OTB1 OTU1 Deubiquitinating enzyme OTUB1 OTU domain-containing ubiquitin aldehyde-binding protein 1 Otubain-1 Ubiquitin-specific-processing protease OTUB1 HSPC263 Otubain-1 protein protein human Homo sapiens Human MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK DIP protein Q96FW1-1 original sequence version: 98 DIP protein Q96FW1-1 original sequence version: 106 DIP protein Q96FW1-1 original sequence version: 112 ube2n_human Ubiquitin-conjugating enzyme E2 N Ubiquitin-protein ligase N Ubiquitin carrier protein N Ubc13 Bendless-like ubiquitin-conjugating enzyme UBE2N BLU UbcH13 E2 ubiquitin-conjugating enzyme N protein protein human Homo sapiens Human MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI DIP protein P61088 original sequence version: 137 DIP protein P61088 original sequence version: 105 DIP protein P61088 original sequence version: 60 DIP protein P61088 original sequence version: 64 DIP protein P61088 original sequence version: 85 FACD84D883D77407 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG otub1-ube2n-2 5 14 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Q96FW1-1:p.[Phe138Ala;Tyr170Ala] Y170A/F138A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'Y170A/F138A'. Wed May 02 19:29:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe138ala,tyr170ala' Tue Jun 30 17:18:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe138Ala;Tyr170Ala]' ecolx Escherichia coli direct interaction direct interaction ube2n-ube2v1-5 6 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction otub1-ube2n-3 4 11 unspecified role unspecified role prey prey ecolx Escherichia coli 14 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli association association ub-10 7 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction otub1-ube2n-4 10 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association otub1-ube2n-5 2 14 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association otub_1-ube2n-1 1 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction otub_1-ube2n-2 3 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 14 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction otub1-ub-1 9 15 unspecified role unspecified role prey prey ecolx Escherichia coli 14 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli association association otub1-ub-2 8 11 unspecified role unspecified role prey prey ecolx Escherichia coli 15 unspecified role unspecified role prey prey ecolx Escherichia coli 14 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli association association