DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. DIP original experiment: DIP-251547X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang M., Mishra S., Sakthivel R., Rojas M., Ranjan R., Sullivan WJ., Fontoura BM., Ménard R., Dever TE., Nussenzweig V. DIP original experiment: DIP-251548X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx PK4, a eukaryotic initiation factor 2α(eIF2α) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. c6ktb8_plaf7 PF3D7_0628200 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MCNFIKKGIRFNGDKYIFLFDIFIKKYLLNVFVANILWEEENNICFLNRLKNKRKKSILFLKEEYLRYLMILNKEEKNKKKRLKKIYECIKVFSIKEFRWLINKLEIIYFYFFCHLLLLCIFQNIFLLTYMSKEYFLKNIHDYMINILSNDIKFNTCIEFTHNDKYEQKCITMAYYDFLLNKKGKLKKRNKYYDIKNIEPLTGKDKNLYSYYNFSPFFPMSSSDIINVNTNDKISNILWNDKYIDTLNHVNMKKKDIPLNIHSNNILMNNYAYRKYVRSYMIKKRINDLILYNLKNIFEKINNIEKLTNINNFMNFKKEYEKVSKEVCNNNNNNYDPSDYMLILPNSSKDHALYNNINNDEYMYKKFCNFISQAREIKKQREKEKCHRDEKCDRGENYDRGEKYDRDEKYDKEEKGLKEDNYRDVNEWNSNKSERCNKIYPSDYKFFLSEDKKYDTPYYSNERVDFHNSDIYMNDMDEELYYSSYHNNHHNNDITYNSNVYKRMLNEVIHPSVESNDVKKGPLNNDNIKNKESNVYEINDIIYQLNGEKKMNTNMCEKRGNKIFDSNNFHNINERKKKILDENDMITIDNNIDKKENILFPYFHMEILKDNEMNITKYYKDKQYYGQYKYDENIYIYDLIVLDTSGYIYKVSTDGSYHWKRKIVDHIQDYSNIEEKDDERKVLKKRNKLNEKDMYESNNKYESNMYDMNTYESNKYDINTYDKNISDSTKAHQNIYVLKKNVYDMNYDSKRALKISPYHNYFNTSIINSINRDKLKHSKPLYKNNIKTNDDNKKLRENKTKNKRLVSNYLGNLFYINENNEVIPLNINIKDVVNNSPFKSPLFPNMVFIGSRESTIINLDYDTGHVLRKYDEASNELLDKPPKKKSRKNKSIKNVKDINDNMKLYKEGSQNVEELSFTEEENKKKSILEGEKIEVASLNNEENMNKGFVCEKDDKINNYDNDEDDLFYEQYEDNNDNDNNKNDNNKNDNNKNDNNKNDNNNNNNNNNNNSYYYYNINGNASDDILVHSKNELLNNTYINKTNIKDENSNNNESFLNKIDGMNNFKLLPKRNMKRNRKKNLLKRLMVSTNKLSMLLNRYHLINRSLERMRKDIKRGKNKRILKKRQLQISLIKWVIKAVDENTLKKKWITTWVDVGSIFMTDVHRKDTSFINSLIEIVGNKLILRPIEKDKMKSTTYQILKIMNNDTDEIQMYRNEDMKYEVINNVDDINNVDNINNANNMNNVNNINNMNNMNNMNNMNNMNNMNNINNMNNINNMNNINNINNINNMNNILNDRLKNRINNKDNSNIKSKIFIFSESISSVFAVKYKNLSNIFTLDIIAKPNIKLYSDYDNLNNFTYNPVHVKKERTLFLPFSKDISDLDGDKFSCSFEDNIIYGKRLIHRLNSISVNISSIEKDMKYLLSNIIYVYDKNKRIPISYIYDMKNLIYEYQKVKQEFLYHLPWDEGDQKYLSRTDDVLNNSIIDNIGKKGPIFICEYINKFMDLYFEENDICYDYCSMLNIWDKIFNNTVSDGDSLLLSNLYRVVHNAFKNNNKYNNNNIYFDNNINININSSSSSSSRSRRNIYNFDNYYNNRRDYNSFWEDRHNILMNRENFLINTSTDKVFSGGKNNELKEFTSIRYKRRRWYWRVFYTIMFIIFFPVLFIYRRIIKRRKGSSKGNKIGTSSNNKLIKKNRTFKDYEDDENNIMSEDEEDDLDMNYDLIFDDDRLKVKKIKRMRKNYNNNNNNNNNKNNNNISNNNSNSNSKSNRFLSKLSNIDLANIDLNLIKKSHGKKMDNFEQPTLVDILARHARDSTHNDGVSYYPFNENETYNMLSLNYAWGGNHKHMNVERTSEYNMGNISHQLNYNNIRNLGDNKISAYELDIYEKELFHLYRRRAASQDVLNKKSFVMKKRIRSSYKVGSSNKYHKKNYTDNEKDKKKYRSYKEKHINEKMFDKKEFLNFLTNFNKKFMKKNSLVDHLIKMNDKAEDNYDGYNSSGSRYNNINDDGVELCGTKRYTNNKNNSDYDNYNNNNNMKNKRYSNKKHNNDNIIINNNNNKYTDERKYRNKSIKEDVDYTNDYYNIQLNNNKINNNQTKNKIDTIRNISHEKLGNNKSSSARNLSLIQTSHIPYDAPLADFLENGRFLRTFENISLIGQGGFGSVYKVSHRLEPGSPTYAVKFIYLKVSSLDNVSSRRYFREIAANRDIYSKHVVRYYTWWCEEPQFLPMHLMPKEIQNLVKKNKDTFKKRLTKNKKYSNNCISDSSNNNNSSCYSASSYNSSINSNYRNMKLWIKKKEQSPDMKRYKEVLRKNNAPNLVFYSDNDGLTSKNKENPEKNHNPFLSDKNFSDSIYKKKKSHDYNSSSHKLKKRKNKKKKSKKKRKSKSKIKTNAQGIYEESENDEGRDHFQYKKGKEQFSKFIGKHNSMGFTQSFQEYDPFDNGYLSEEDRDLIVFADNEESNGNDQQMIRHDNMNNENVIIKHRNEDDKNGLDGDKNGLDGDKNGLDGDKNGLDGDKNGLDGDKNELDGDKNGLDGDKNGLDGDKNGLDGDKNELDDNTKKLDDNTKKLDDLLMKQKINSLTRNDIVNIENENPAPHATNNIKNKKVDLNGELTYYDYVGKNEVIPNSRTETNVESINTNGMFNNKFSVMKDEGGEYKKKENMTWGDTKRDGLYENGKHEKDGLGVNKCITNKYIENDDDDDDDDDNNNNNNNIDERKKDLKKKQKNAITKGNEDLLATNGTNNKEKRKKDDDINKNMEKIKSYKKKTPVPEFSIVLLLQMELCKGYTLRKWLDRSTRSDKPLHFTYSDKKMNHPLEFDLFKQLIKGLKDIHATCFIHRDLKPENIFVDPDTYTLKIGDLGLVRFIEEKKREKDFNNIDCYKDNIYTDINQNAITSQISIKGQIIGTPGYTAPEGGALCDEKADIYSAALILLELLCPRFTTIMERYKRLNDFRNYYTVPDYVKIHLNPWYILMLQMSKPNPADRPSAADVYSKIKVLLDPHLTDFAFSFNDIHNEHMNKPPQGTNNFERITDNKDKFVIQSVVDMKNKVENEEIPIEKGLNSNVENIKNENNGADK DIP protein C6KTB8 original sequence version: 31 86C96D7EE0256EDC q8ibh7_plaf7 PF3D7_0728000 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MTEMRVKADLGDCRFYKKKFPEVDDLIMVKVNRIEDMGAYVSILEYNDMEGMILMSELSKRRFRSVNKLIRVGRHEVVLVLRVDSQKGYIDLSKRRVSPKDIIKCEEKFSKSKKVHQTVRHVAKEHGITVEELNEKAIWPLYERYGHALDALKEATMNPENVFKGLDISEEIKNSLLKDIKLRLTPQALKLRGRIDVWCFGYEGIDAVKEALKKGKEISNDKVSINIKLIAPPQYVIVTSCHDKDLGMAKIQEAMKVISDKIKEYKGGDFKQQGEILVIGGDEEKRLEELLDKHDGISSDDDDYNTSDEDDENSSEEDENTSEDEEEED DIP protein Q8IBH7 original sequence version: 62 8A0237A13C5B7733 c6ktb8_plaf7-1 1 3 putative self putative self unspecified role unspecified role Region 2109-2236/2758-3072 Region 2109-2236/2758-3072 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true c6ktb8_plaf7-q8ibh7_plaf7-1 2 4 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 3 enzyme enzyme unspecified role unspecified role Region 2109-2236/2758-3072 Region 2109-2236/2758-3072 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation