DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250783X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250779X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250780X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250782X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250781X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. Nature structural & molecular biology 2012 imex curation Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Dou H., Buetow L., Hock A., Sibbet GJ., Vousden KH., Huang DT. DIP original experiment: DIP-250784X Nature structural & molecular biology 2012 IMEx Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl. zap70_human Tyrosine-protein kinase ZAP-70 ZAP70 SRK Syk-related tyrosine kinase 70 kDa zeta-chain associated protein protein protein human Homo sapiens Human MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA DIP protein P43403 original sequence version: 146 DIP protein P43403-1 original sequence version: 167 D1E1A8EC66FA116F ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM DIP protein P62837 original sequence version: 81 DIP protein P62837-1 original sequence version: 134 DIP protein P62837 original sequence version: 95 DIP protein P62837 original sequence version: 139 DIP protein P62837 original sequence version: 37 DIP protein P62837 original sequence version: 115 DIP protein P62837 original sequence version: 58 DIP protein P62837 original sequence version: 74 DIP protein P62837 original sequence version: 103 DIP protein P62837 original sequence version: 101 C942BE7853CBC355 cbl_human E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-Cbl Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 CBL CBL2 RNF55 RING-type E3 ubiquitin transferase CBL protein protein human Homo sapiens Human MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DIP protein P22681 original sequence version: 173 DIP protein P22681 original sequence version: 128 DIP protein P22681 original sequence version: 145 DIP protein P22681 original sequence version: 150 DIP protein P22681 original sequence version: 191 DIP protein P22681 original sequence version: 91 DIP protein P22681 original sequence version: 139 1AA6BF67377322CA cbl-zap70-1 2 7 unspecified role unspecified role unspecified role unspecified role Region 286-297 Region 286-297 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role unspecified role unspecified role Region 47-435 Region 47-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cbl-ube2d2-1 5 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 354-435 Region 354-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cbl-zap70-2 1 7 unspecified role unspecified role prey prey Region 286-297 Region 286-297 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait Region 47-435 Region 47-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction cbl-ube2d2-2 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 286-297 Region 286-297 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role unspecified role unspecified role Region 47-435 Region 47-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association cbl-ube2d2-3 4 8 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait Region 354-435 Region 354-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P22681:p.Met222Glu M222E mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M E This feature has been corrected as a result of our quality control procedures. The original label was 'M222E'. Wed May 02 14:48:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met222glu' Tue Jun 30 16:39:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met222Glu' P22681:p.Tyr368Phe Y368F mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y F This feature has been corrected as a result of our quality control procedures. The original label was 'Y368F'. Wed May 02 14:33:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr368phe' Tue Jun 30 19:06:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr368Phe' pY371 pY371 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cbl-ube2d2-4 6 8 unspecified role unspecified role prey prey P62837:p.Lys4Ala K4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K4A'. Wed May 02 15:53:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys4ala' Tue Jun 30 19:32:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys4Ala' ecolx Escherichia coli 9 unspecified role unspecified role bait bait Region 354-435 Region 354-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction