DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255502X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255505X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255503X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255500X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255501X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Phosphorylation-dependent activity of the deubiquitinase DUBA. Nature structural & molecular biology 2012 imex curation Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Huang OW., Ma X., Yin J., Flinders J., Maurer T., Kayagaki N., Phung Q., Bosanac I., Arnott D., Dixit VM., Hymowitz SG., Starovasnik MA., Cochran AG. DIP original experiment: DIP-255504X Nature structural & molecular biology 2012 IMEx Phosphorylation-dependent activity of the deubiquitinase DUBA. q96g74-1 OTU domain-containing protein 5 OTUD5 Deubiquitinating enzyme A protein protein human Homo sapiens Human MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRDPPPDKS DIP protein Q96G74-1 original sequence version: 77 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG otud5-ub-1 4 8 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal 7 enzyme enzyme unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction otud5-ub-2 1 8 enzyme target enzyme target substrate substrate unspecified role unspecified role K11 K11 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain K48 K48 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain K63 K63 ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS177 pS177 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli deubiquitination deubiquitination reaction deubiquitination otud5-ub-3 6 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS177 pS177 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction otud5-ub-4 5 8 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal 7 enzyme enzyme unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS177 pS177 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction otud5-ub-5 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS177 pS177 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction otud5-ub-6 2 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role OTU domain Region 172-351 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS177 pS177 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction