DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249254X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249257X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249251X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249255X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249256X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249253X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. Structure (London, England : 1993) 2012 imex curation Du J., Kelly AE., Funabiki H., Patel DJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du J., Kelly AE., Funabiki H., Patel DJ. DIP original experiment: DIP-249252X Structure (London, England : 1993) 2012 IMEx Structural basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human Survivin. birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD DIP protein O15392 original sequence version: 129 DIP protein O15392 original sequence version: 132 DIP protein O15392-1 original sequence version: 141 DIP protein O15392-1 original sequence version: 143 9E7CADCDF2822286 h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA smacn Diablo peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AVPIAQKSEPHSLSS birc5-smacn-1 6 10 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction birc5-h3(121)-1 3 9 unspecified role unspecified role unspecified role unspecified role Region 1-10 Region 1-10 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction birc5-smacn-2 5 10 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role O15392:p.[Lys62Tyr;His80Trp] K62Y/H80W mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H W certain certain sequence position certain certain certain sequence position certain K Y This feature has been corrected as a result of our quality control procedures. The original label was 'K62Y/H80W'. Wed May 02 18:36:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys62tyr,his80trp' Tue Jun 30 20:18:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys62Tyr;His80Trp]' ecolx Escherichia coli direct interaction direct interaction birc5-h3(121)-2 1 9 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction birc5-h3(121)-3 7 9 unspecified role unspecified role unspecified role unspecified role pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction birc5-h3(121)-4 2 9 unspecified role unspecified role unspecified role unspecified role pT3 pT3 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role O15392:p.Glu63Ala E63A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E63A'. Wed May 02 16:02:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu63ala' Tue Jun 30 18:35:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu63Ala' O15392:p.Leu64Ala L64A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L64A'. Wed May 02 16:28:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu64ala' Tue Jun 30 18:59:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu64Ala' O15392:p.Trp67Ala W67A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W67A'. Wed May 02 18:45:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp67ala' Tue Jun 30 17:07:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp67Ala' O15392:p.His80Ala H80A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H80A'. Wed May 02 16:29:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his80ala' Tue Jun 30 19:47:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His80Ala' O15392:p.[Lys62Tyr;His80Trp] K62Y/H80W mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H W certain certain sequence position certain certain certain sequence position certain K Y This feature has been corrected as a result of our quality control procedures. The original label was 'K62Y/H80W'. Wed May 02 15:02:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys62tyr,his80trp' Tue Jun 30 18:51:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys62Tyr;His80Trp]' O15392:p.Glu76Ala E76A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E76A'. Wed May 02 15:24:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu76ala' Tue Jun 30 20:16:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu76Ala' O15392:p.Asp71Ala D71A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D71A'. Wed May 02 14:42:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp71ala' Tue Jun 30 19:33:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp71Ala' O15392:p.Glu65Ala E65A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E65A'. Wed May 02 19:34:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu65ala' Tue Jun 30 19:27:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu65Ala' ecolx Escherichia coli direct interaction direct interaction h3(121)-birc5-1 4 9 unspecified role unspecified role bait bait Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction