DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249246X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249244X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249247X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249249X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249250X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249248X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. Structure (London, England : 1993) 2012 imex curation Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Graveline R., Kumar GS., Zhang Y., Krishnan A., David G., Radhakrishnan I. DIP original experiment: DIP-249245X Structure (London, England : 1993) 2012 IMEx Structural basis for molecular interactions involving MRG domains: implications in chromatin biology. phf12_mouse PHD finger protein 12 Phf12 Kiaa1523 PHD factor 1 protein protein mouse Mus musculus Mouse MWEKMETKTIVYDLDTSGGLMEQIQALLAPPKTDEAEKRSRKPEKESRRSGRATNHDSCDSCKEGGDLLCCDHCPAAFHLQCCNPPLSEEMLPPGEWMCHRCTVRRKKREQKKELGHVNGLVDKSSKRTTSPSSDTDLLDRPASKTELKAIAHARILERRASRPGTPTSNASTETPTSEHNDVDEDIVDVDEEPVAAEPDYVQPQLRRPFELLIAAAMERNPTQFQLPNELTCTTALPGSSKRRRKEETTGKNVKRTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQCDYCPLLFHMDCLEPPLTAMPLGRWMCPNHIEHVVLNQKNLTLSNRCQVFDRFQDTISQHVVKVDFLNRIHKKHPPNRRVLQSVKRRSLKVPDAIKSQYQFPPPLIAPAAIRDGELICSGVPEESQTHLLNSEHLATQAEQQEWLCSVVALQCSILKHLSAKQMPSPWDSEQTEKADIKPVIVTDSSITTSLQTADKAPLPSHYPLSCPSAVSTQNSLGCSPPHQPPTLEDISCSSCVEKSKKAPCGTANGPVNTEIKANGPHLYSSPTDSTDPRRLPGANTPLPGLTHRQGWPRPLTPPSAGGLQNHVGIIVKTENATGPSSCPQRSLVPVPSLPPSIPSSCASIENTSTLHRKTVQSQIGPSSTESRPLGSPPNATRVLTPPQAAGDSILATGANQRFCSPAPSSDGKVSPGTLSIGSALTVPSFPANSTAMVDLTNSLRAFMDVNGEIEINMLDEKLIKFLALQRVHQLFPSRVQASPGNVGTHPLASGGHHPEVQRKEVQARAVFCPLLGLGGAVNMCYRTLYIGTGADMDVCLTNYGHCNYVSGKHACIFYDENTKHYELLNYSEHGTTVDNVLYSCDFSEKTPPTPPSSIVAKVQSVIRRRRHQKQDEEPSEEAAMMSSQAQGPQRRPCNCKASSSSLIGGSGAGWEGTALLHHGSYIKLGCLQFVFSITEFATKQPKGDASLLQDGVLAEKLSLKPHQGPVLRSNSVP DIP protein Q5SPL2 original sequence version: 60 palb2_human Partner and localizer of BRCA2 PALB2 FANCN protein protein human Homo sapiens Human MDEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQDLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRTDDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEEISSKNPARSPVTEIRTHLLSLKSELPDSPEPVTEINEDSVLIPPTAQPEKGVDTFLRRPNFTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKNIRFTSPVSLEAQGKKMTVSTDNLLVNKAISKSGQLPTSSNLEANISCSLNELTYNNLPANENQNLKEQNQTEKSLKSPSDTLDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQRKVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQKLLSLTKVSSPAGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVNRSKEEVTSHKYQHEKLFIQVKGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSLKQLLSFLSITDFQLPDEDFGPLKLEKVKSCSEKPVEPFESKMFGERHLKEGSCIFPEELSPKRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSSSILLYTPLNTVAPDDNDRPTTDMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLASDTKQFDSSGSPAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTETAELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKALDAWQWEKLYTWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVLLKSGNIKAVLGLTKRRLVSSSGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQGMQEALLGTTIMNNIVIWNLKTGQLLKKMHIDDSYQASVCHKAYSEMGLLFIVLSHPCAKESESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLEGDVKDHCAAAILTSGTIAIWDLLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYHYS DIP protein Q86YC2 original sequence version: 96 DIP protein Q86YC2 original sequence version: 62 DIP protein Q86YC2 original sequence version: 65 DIP protein Q86YC2 original sequence version: 107 DIP protein Q86YC2 original sequence version: 74 DIP protein Q86YC2 original sequence version: 112 215EC32291315FA2 q9ubu8-2 Mortality factor 4-like protein 1 MORF4L1 MORF-related gene 15 protein MRG15 Protein MSL3-1 Transcription factor-like protein MRG15 FWP006 HSPC008 HSPC061 PP368 protein protein human Homo sapiens Human MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKVAIKDKQVKYFIHYSGWNKNWDEWVPESRVLKYVDTNLQKQRELQKANQEQYAEGKMRGAAPGKKTSGLQQKNVEVKTKKNKQKTPGNGDGGSTSETPQPPRKKRARVDPTVENEETFMNRVEVKVKIPEELKPWLVDDWDLITRQKQLFYLPAKKNVDSILEDYANYKKSRGNTDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILADHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLNYLHDFLKYLAKNSATLFSASDYEVAPPEYHRKAV DIP protein Q9UBU8-2 original sequence version: 161 DIP protein Q9UBU8-2 original sequence version: 126 DIP protein Q9UBU8-2 original sequence version: 122 33CDBEC7D9CF513F phf12_human PHD finger protein 12 KIAA1523 PHD factor 1 PHF12 protein protein human Homo sapiens Human MWEKMETKTIVYDLDTSGGLMEQIQALLAPPKTDEAEKRSRKPEKEPRRSGRATNHDSCDSCKEGGDLLCCDHCPAAFHLQCCNPPLSEEMLPPGEWMCHRCTVRRKKREQKKELGHVNGLVDKSGKRTTSPSSDTDLLDRSASKTELKAIAHARILERRASRPGTPTSSASTETPTSEQNDVDEDIIDVDEEPVAAEPDYVQPQLRRPFELLIAAAMERNPTQFQLPNELTCTTALPGSSKRRRKEETTGKNVKKTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQCDYCPLLFHMDCLEPPLTAMPLGRWMCPNHIEHVVLNQKNMTLSNRCQVFDRFQDTVSQHVVKVDFLNRIHKKHPPNRRVLQSVKRRSLKVPDAIKSQYQFPPPLIAPAAIRDGELICNGIPEESQMHLLNSEHLATQAEQQEWLCSVVALQCSILKHLSAKQMPSHWDSEQTEKADIKPVIVTDSSVTTSLQTADKTPTPSHYPLSCPSGISTQNSLSCSPPHQSPALEDIGCSSCAEKSKKTPCGTANGPVNTEVKANGPHLYSSPTDSTDPRRLPGANTPLPGLSHRQGWPRPLTPPAAGGLQNHTVGIIVKTENATGPSSCPQRSLVPVPSLPPSIPSSCASIENTSTLQRKTVQSQIGPPLTDSRPLGSPPNATRVLTPPQAAGDGILATTANQRFSSPAPSSDGKVSPGTLSIGSALTVPSFPANSTAMVDLTNSLRAFMDVNGEIEINMLDEKLIKFLALQRIHQLFPSRVQPSPGSVGTHQLASGGHHIEVQRKEVQARAVFYPLLGLGGAVNMCYRTLYIGTGADMDVCLTNYGHCNYVSGKHACIFYDENTKHYELLNYSEHGTTVDNVLYSCDFSEKTPPTPPSSIVAKVQSVIRRRRHQKQDEEPSEEAAMMSSQAQGPQRRPCNCKASSSSLIGGSGAGWEGTALLHHGSYIKLGCLQFVFSITEFATKQPKGDASLLQDGVLAEKLSLKPHQGPVLRSNSVP DIP protein Q96QT6 original sequence version: 88 239223BE9EAF619B phf12-morf4l1-2 5 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9UBU8-2:p.Val227Ala V227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V227A'. Wed May 02 17:57:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val227ala' Tue Jun 30 17:33:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val227Ala' Q9UBU8-2:p.Leu287Ala L287A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L287A'. Wed May 02 18:39:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu287ala' Tue Jun 30 16:16:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu287Ala' Q9UBU8-2:p.Leu168Ala L168A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L168A'. Wed May 02 17:10:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu168ala' Tue Jun 30 18:15:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu168Ala' human Homo sapiens Human physical association physical association morf4l1-phf12-1 2 11 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction morf4l1-phf12-2 6 11 unspecified role unspecified role unspecified role unspecified role Region 219-232 Region 219-232 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction morf4l1-palb2-2 3 9 unspecified role unspecified role unspecified role unspecified role Region 562-629 Region 562-629 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction morf4l1-phf12-3 1 11 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q96QT6:p.Phe210Ala F210A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F210A'. Wed May 02 19:21:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe210ala' Tue Jun 30 18:05:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe210Ala' Q96QT6:p.Phe225Ala F225A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:06:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe225ala' Tue Jun 30 19:32:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe225Ala' Q96QT6:p.Leu213Ala L213A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L213A'. Wed May 02 20:22:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu213ala' Tue Jun 30 19:16:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu213Ala' Q96QT6:p.Leu227Ala L227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L227A'. Wed May 02 17:01:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu227ala' Tue Jun 30 16:25:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu227Ala' Q96QT6:p.Pro228Ala P228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P228A'. Wed May 02 19:05:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro228ala' Tue Jun 30 20:02:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro228Ala' Q96QT6:p.Asn221Glu N221E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N E This feature has been corrected as a result of our quality control procedures. The original label was 'N221E'. Wed May 02 20:27:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn221glu' Tue Jun 30 17:43:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn221Glu' ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction morf4l1-phf12-4 7 11 unspecified role unspecified role unspecified role unspecified role Region 200-241 Region 200-241 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Q9UBU8-2:p.Val268Ala V268A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V268A'. Wed May 02 19:55:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val268ala' Tue Jun 30 18:21:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val268Ala' Q9UBU8-2:p.Val227Ala V227A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V227A'. Wed May 02 14:59:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val227ala' Tue Jun 30 17:27:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val227Ala' Q9UBU8-2:p.Leu287Ala L287A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L287A'. Wed May 02 16:31:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu287ala' Tue Jun 30 18:38:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu287Ala' Q9UBU8-2:p.Tyr235Ala Y235A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y235A'. Wed May 02 15:13:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr235ala' Tue Jun 30 19:59:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr235Ala' Region 155-323 Region 155-323 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9UBU8-2:p.Met228Ala M228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M228A'. Wed May 02 17:15:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met228ala' Tue Jun 30 19:12:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met228Ala' Q9UBU8-2:p.Met273Ala M273A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M273A'. Wed May 02 18:29:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met273ala' Tue Jun 30 17:02:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met273Ala' Q9UBU8-2:p.Trp172Ala W172A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W172A'. Wed May 02 20:10:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp172ala' Tue Jun 30 17:12:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp172Ala' ecolx Escherichia coli direct interaction direct interaction phf12-morf4l1-3 4 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5SPL2:p.Phe210Ala F210A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F210A'. Wed May 02 15:17:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe210ala' Tue Jun 30 19:23:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe210Ala' Q5SPL2:p.Phe225Ala F225A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:28:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe225ala' Tue Jun 30 17:03:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe225Ala' Q5SPL2:p.Leu213Ala L213A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L213A'. Wed May 02 17:03:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu213ala' Tue Jun 30 19:22:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu213Ala' Q5SPL2:p.Leu227Ala L227A mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L227A'. Wed May 02 15:53:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu227ala' Tue Jun 30 16:30:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu227Ala' Q5SPL2:p.Pro228Ala P228A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P228A'. Wed May 02 20:29:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro228ala' Tue Jun 30 17:32:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro228Ala' human Homo sapiens Human 10 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association