DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure (London, England : 1993) 2011 imex curation Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220179X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure (London, England : 1993) 2011 imex curation Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220176X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure (London, England : 1993) 2011 imex curation Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220177X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Structure (London, England : 1993) 2011 imex curation Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220178X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. q01344-2 Interleukin-5 receptor subunit alpha IL5RA IL5R Soluble-S1 CDw125 protein protein human Homo sapiens Human MIIVAHVLLILLGATEILQADLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGFSR DIP protein Q01344-2 original sequence version: 120 il5_human Interleukin-5 IL5 T-cell replacing factor Eosinophil differentiation factor B-cell differentiation factor I protein protein human Homo sapiens Human MRMLLHLSLLALGAAYVYAIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES DIP protein P05113 original sequence version: 130 DC984467179556A3 il5-il5ra-3 1 5 unspecified role unspecified role prey prey Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role bait bait P05113:p.Arg110Ala R110A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R110A'. Wed May 02 17:53:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg110ala' Tue Jun 30 19:57:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg110Ala' ecolx Escherichia coli direct interaction direct interaction il5-il5ra-4 2 5 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction il5-il5ra-5 3 5 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction il5-il5ra-6 4 5 unspecified role unspecified role prey prey Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q01344-2:p.Glu78Pro E78P mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E P This feature has been corrected as a result of our quality control procedures. The original label was 'E78P'. Wed May 02 15:21:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu78pro' Tue Jun 30 19:49:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu78Pro' Q01344-2:p.Asp75Glu D75E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D E This feature has been corrected as a result of our quality control procedures. The original label was 'D75E'. Wed May 02 15:47:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp75glu' Tue Jun 30 18:33:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp75Glu' Q01344-2:p.Arg80Ala R80A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R80A'. Wed May 02 17:25:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg80ala' Tue Jun 30 19:55:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg80Ala' Q01344-2:p.Ser84Ala S84A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S84A'. Wed May 02 17:11:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser84ala' Tue Jun 30 18:52:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser84Ala' Q01344-2:p.Ile89Ala I89A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I89A'. Wed May 02 19:38:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile89ala' Tue Jun 30 18:10:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile89Ala' Q01344-2:p.Trp178Ala W178A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W178A'. Wed May 02 15:12:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp178ala' Tue Jun 30 17:01:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp178Ala' Q01344-2:p.Ile203Ala I203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I203A'. Wed May 02 15:21:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile203ala' Tue Jun 30 15:59:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile203Ala' Q01344-2:p.Arg208Lys R208K mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R K This feature has been corrected as a result of our quality control procedures. The original label was 'R208K'. Wed May 02 15:36:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg208lys' Tue Jun 30 16:09:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg208Lys' Q01344-2:p.Asp228Ala D228A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D228A'. Wed May 02 15:40:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp228ala' Tue Jun 30 17:10:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp228Ala' Q01344-2:p.Met315Ala M315A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M315A'. Wed May 02 17:02:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met315ala' Tue Jun 30 16:49:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met315Ala' Q01344-2:p.Leu43Ala L43A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L43A'. Wed May 02 18:27:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu43ala' Tue Jun 30 18:48:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu43Ala' Q01344-2:p.Tyr77Ala Y77A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y77A'. Wed May 02 14:46:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr77ala' Tue Jun 30 16:26:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr77Ala' ecolx Escherichia coli 6 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction