DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253820X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253822X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253818X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253821X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253817X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Sitar T., Gallinger J., Ducka AM., Ikonen TP., Wohlhoefler M., Schmoller KM., Bausch AR., Joel P., Trybus KM., Noegel AA., Schleicher M., Huber R., Holak TA. DIP original experiment: DIP-253819X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 q9u1k1-1 Protein spire p150-Spir CG10076 spir protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK DIP protein Q9U1K1-1 original sequence version: 83 DIP protein Q9U1K1 original sequence version: 83 DIP protein Q9U1K1 original sequence version: 99 010CD024F9ABC078 act1_drome Actin-5C Act5C CG4027 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P10987 original sequence version: 117 DIP protein P10987 original sequence version: 132 BDAF13C191BB1BBC act5c-spir-4 5 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-spir-5 6 7 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit 8 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 396-450 Region 396-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-spir-6 3 7 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit 8 unspecified role unspecified role unspecified role unspecified role Region 428-485 Region 428-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 396-450 Region 396-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction act5c-spir-5 1 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role Region 396-485 Region 396-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-spir-7 2 7 unspecified role unspecified role unspecified role unspecified role Pyrene-label Pyrene-label pyrene label pyrene label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit 8 unspecified role unspecified role unspecified role unspecified role Region 448-485 Region 448-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction act5c-spir-6 4 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role Region 371-388 Region 371-388 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 396-419 Region 396-419 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 428-450 Region 428-450 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 461-485 Region 461-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction