DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220454X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220453X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220456X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220451X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220457X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220455X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. Nature structural & molecular biology 2011 imex curation Moser BA., Chang YT., Kosti J., Nakamura TM. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Moser BA., Chang YT., Kosti J., Nakamura TM. DIP original experiment: DIP-220452X Nature structural & molecular biology 2011 IMEx Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast. tpz1_schpo Protection of telomeres protein tpz1 tpz1 mug169 Meiotically up-regulated gene 169 protein SPAC6F6.16c SPAC6F6.18c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNCLKHPWLENGLLNLIKNADVLPIRVFKCQPLQIFEYIRYEHPIRCKLSDTEFYIEAEFSSQSISDLNNFTEKRITSLRGGIVTLGNFLIHLIPSQSGIIPWIQVESFNFQGCEGAVFGNPKAITTSALFNALLQSPYLAALANEFNRSIKEGSSYQEASLSQQEKPNDNTSNSRDIKNNIQFHWKNMTSLSIEECIIPKGQQLILEKESEENTTHGIYLEERKMAQGLHNSVSETPEVKQEDNDEDLDAYSWSSSTDSAGEIPSLPTNRKILEKIAEKPPPFESPLEDDETPDQTNEHEANQVNVSQLPLNPRGSGISGRPVESTEQLNSSLTIERSQSIQSTDSKQRVETQSHRRSKIEIFDAQDELFDRSICTTIDDSTGKLLNAEETPIKTGDLHSTSASSVISCTPPAINFTSDICNEQIELEYKRKPIPDYDFMKGLETTLQELYVEHQSKKRRLELFQLTNNHQKNSEACEMCRLGLPHGSFFELLRDWKKIEEFRNKS A1A2F59238DACBF7 A1A2F59238DACBF7 clr3_schpo Histone deacetylase clr3 clr3 Cryptic loci regulator 3 SPBC800.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLASNSDGASTSVKPSDDAVNTVTPWSILLTNNKPMSGSENTLNNESHEMSQILKKSGLCYDPRMRFHATLSEVDDHPEDPRRVLRVFEAIKKAGYVSNVPSPSDVFLRIPAREATLEELLQVHSQEMYDRVTNTEKMSHEDLANLEKISDSLYYNNESAFCARLACGSAIETCTAVVTGQVKNAFAVVRPPGHHAEPHKPGGFCLFNNVSVTARSMLQRFPDKIKRVLIVDWDIHHGNGTQMAFYDDPNVLYVSLHRYENGRFYPGTNYGCAENCGEGPGLGRTVNIPWSCAGMGDGDYIYAFQRVVMPVAYEFDPDLVIVSCGFDAAAGDHIGQFLLTPAAYAHMTQMLMGLADGKVFISLEGGYNLDSISTSALAVAQSLLGIPPGRLHTTYACPQAVATINHVTKIQSQYWRCMRPKHFDANPKDAHVDRLHDVIRTYQAKKLFEDWKITNMPILRDSVSNVFNNQVLCSSNFFQKDNLLVIVHESPRVLGNGTSETNVLNLNDSLLVDPVSLYVEWAMQQDWGLIDINIPEVVTDGENAPVDILSEVKELCLYVWDNYVELSISKNIFFIGGGKAVHGLVNLASSRNVSDRVKCMVNFLGTEPLVGLKTASEEDLPTWYYRHSLVFVSSSNECWKKAKRAKRRYGRLMQSEHTETSDMMEQHYRAVTQYLLHLLQKARPTSQ 6B0E4184A056D899 ccq1_schpo Coiled-coil quantitatively-enriched protein 1 ccq1 Structural maintenance of chromosomes protein ccq1 SPCC188.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEINDFTNSIIINESDTKYEVYSEVNSSNRWIAKEDCPSPLEDVWLLKLAGHKRKLEEPLSCMRNEEPASKQREIEKFLRTDLDHSENDFLTQEVDEFPSTQQLFPIPLQNDTAFDSSEESITKSSKSSFSVLDIGLPMSALQRKMMHRLVQYFAFCIDHFCTGPSDSRIQEKIRLFIQSAHNIAKHPSLYDTEVRNPSAAESTNSHVSLDASNFSSYAENSSKFLFLQELFKNLSPSYSKTFFLFISNQFLANTLTQWLKSQNIDAELWAEEDAKTSQHPAIWICVSKKAPSASHFLQSCPDLSATIFYDIEAYMSVTSSLPSIQSLVLRLIHLGSIEHAIKCFQSSYNASFLVNIVGVVATLSSSSEENSEASNLSTLFEKSGNFEEILGSESHSSITEKTRDIAKNVATWLKNGENFSSWPLPPLMDLASLSVAEPRDSQPSVSQVNDTFVKSSDSTFPSSQSMQSPSKLHSLTSNATDLLSSSSLKKNFFSQQEADEVELSNNYDLQGAAVQYLQRRLRMVEDELHEAINSKNVQQSRSEELEQQISKLTDNLQEYRNTVRELKLDLEKSKKKNEDLSKLEVEKVEEIANLKKELTHLAKQQEFGFKYVQEFSNEDLQGKLIEANEKNYKLTQLLKTQKEDADFITNQYQNASTFAAEQSKEVAKLQAECKRLQAINSKVMEEVKVYNDSRVEALLAKVSSLEETLKILEQKSLPKFTPHNQSPRIIDSN 14193682700A2634 est1_schpo Telomere elongation protein est1 Ever shorter telomeres protein 1 est1 SPBC2D10.13 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSQQLSSNEICEEQGKLVKALCEAAQGGFKHDLYEKIIAIEIEVEKKLLNRLKSIQTNTFERPDIIWSCCHYKIIQHFRSRFREIHPRHVVEKKKTKKVFFKFLKTCAIFYQTCISELISKFQLDSYRPFFCKWTSSATVSSTISNDEMSSIPEASYSRNHMEALECVYNCFIYLGDMARYSSTCLKKRGAYDRALGFYDLAHRTLPGNGMHRNQIAVVWASDECIVESIYWFSSALCSEDPPKSALLNLLKQLIAFYRRCFAVHFEFVSPMMILLFIISDCCIHSLKEIQPFKCPSIMVKDLENSLLQSNIRNVGYEKKNLYYISSAFSNLLHCRYFNCNDRLRSFYYRFSSWLFHQTLACAKEVESERAPTSYLTSCLPSIKVILARVLESSPAFGFIERNELEQLSYHFRICEKFFKESSENKMLNLFEDYNEFGLCKSFICLFKRLNEDIIGPKLNINPPDYTTHPFSESIKRFYQISKILNLLLS AAB5A05688AA3360 AAB5A05688AA3360 clr3-ccq1-1 2 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association tpz1-ccq1-1 4 8 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey Region 123-436 Region 123-436 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ccq1-est1-1 5 11 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role bait bait Region 86-100 Region 86-100 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pT73 pT73 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Q10432:p.Gln94Ala Q94A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q94A'. Wed May 02 16:41:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln94ala' Tue Jun 30 20:05:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln94Ala' Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q10432:p.Thr93Asp T93D mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T93D'. Wed May 02 18:35:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr93asp' Tue Jun 30 19:11:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr93Asp' in vitro In vitro physical association physical association est1-ccq1-1 1 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey Region 1-436 Region 1-436 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q10432:p.Thr93Asp T93A/D/E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T D Wed May 02 16:43:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr93asp' Tue Jun 30 18:04:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr93Asp' Q10432:p.Gln94Ala Q94A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q94A'. Wed May 02 18:36:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln94ala' Tue Jun 30 16:06:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln94Ala' Q10432:p.Thr93Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:23:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr93ala' Tue Jun 30 17:20:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr93Ala' Q10432:p.Thr93Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T E Wed May 02 17:17:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr93glu' Tue Jun 30 20:13:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr93Glu' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association tpz1-ccq1-2 7 8 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association ccq1-est1-2 3 11 unspecified role unspecified role prey prey O74804:p.Arg79Ala R79A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R79A'. Wed May 02 15:55:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg79ala' Tue Jun 30 15:35:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg79Ala' O74804:p.Arg180Ala R180A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R180A'. Wed May 02 16:56:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg180ala' Tue Jun 30 19:13:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg180Ala' O74804:p.[Arg79Ala;Arg180Ala] R79A/R180A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R79A/R180A'. Wed May 02 17:49:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg79ala,arg180ala' Tue Jun 30 18:11:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg79Ala;Arg180Ala]' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role bait bait Region 86-100 Region 86-100 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pT73 pT73 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro physical association physical association est1-ccq1-2 6 11 unspecified role unspecified role bait bait O74804:p.Lys73Ala K73A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K73A'. Wed May 02 19:12:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys73ala' Tue Jun 30 19:19:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys73Ala' O74804:p.Arg79Ala R79A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R79A'. Wed May 02 15:09:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg79ala' Tue Jun 30 16:45:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg79Ala' O74804:p.Arg180Ala R180A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R180A'. Wed May 02 18:39:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg180ala' Tue Jun 30 16:41:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg180Ala' O74804:p.[Arg79Ala;Arg180Ala] R79A/R180A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R79A/R180A'. Wed May 02 15:20:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg79ala,arg180ala' Tue Jun 30 17:06:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg79Ala;Arg180Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association