DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Structure (London, England : 1993) 2011 imex curation Park YB., Chae J., Kim YC., Cho Y. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Park YB., Chae J., Kim YC., Cho Y. DIP original experiment: DIP-220118X Structure (London, England : 1993) 2011 IMEx Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Structure (London, England : 1993) 2011 imex curation Park YB., Chae J., Kim YC., Cho Y. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Park YB., Chae J., Kim YC., Cho Y. DIP original experiment: DIP-220120X Structure (London, England : 1993) 2011 IMEx Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Structure (London, England : 1993) 2011 imex curation Park YB., Chae J., Kim YC., Cho Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Park YB., Chae J., Kim YC., Cho Y. DIP original experiment: DIP-220117X Structure (London, England : 1993) 2011 IMEx Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Crystal structure of human Mre11: understanding tumorigenic mutations. Structure (London, England : 1993) 2011 imex curation Park YB., Chae J., Kim YC., Cho Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park YB., Chae J., Kim YC., Cho Y. DIP original experiment: DIP-220119X Structure (London, England : 1993) 2011 IMEx Crystal structure of human Mre11: understanding tumorigenic mutations. nbn_human Nibrin Nijmegen breakage syndrome protein 1 Cell cycle regulatory protein p95 NBN NBS1 NBS P95 protein protein human Homo sapiens Human MWKLLPAAGPAGGEPYRLLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKDNSKYGTFVNEEKMQNGFSRTLKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQPPQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTCVVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDPQGHPSTGLKTTTPGPSLSQGVSVDEKLMPSAPVNTTTYVADTESEQADTWDLSERPKEIKVSKMEQKFRMLSQDAPTVKESCKTSSNNNSMVSNTLAKMRIPNYQLSPTKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLPHIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR DIP protein O60934 original sequence version: 65 DIP protein O60934 original sequence version: 84 DIP protein O60934 original sequence version: 96 DIP protein O60934 original sequence version: 131 DIP protein O60934 original sequence version: 133 DIP protein O60934 original sequence version: 128 DIP protein O60934 original sequence version: 99 DIP protein O60934 original sequence version: 85 CD602F09BA73DAB6 mre11_human Double-strand break repair protein MRE11 MRE11 Double-strand break repair protein MRE11A HNGS1 MRE11A Meiotic recombination 11 homolog 1 Meiotic recombination 11 homolog A protein protein human Homo sapiens Human MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHIDALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR DIP protein P49959 original sequence version: 152 DIP protein P49959 original sequence version: 146 DIP protein P49959 original sequence version: 114 DIP protein P49959 original sequence version: 161 DIP protein P49959 original sequence version: 168 DIP protein P49959 original sequence version: 173 DIP protein P49959 original sequence version: 154 DIP protein P49959-1 original sequence version: 127 DIP protein P49959 original sequence version: 96 DIP protein P49959 original sequence version: 115 D94ABFBDDF6106AD mre11-20 3 6 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-411 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P49959:p.Cys146Ala C146A/R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A Wed May 02 16:15:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys146ala' Tue Jun 30 16:52:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys146Ala' region Δ87-117 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ87-117'. P49959:p.Cys146Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C R Wed May 02 17:16:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys146arg' Tue Jun 30 17:23:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys146Arg' ecolx Escherichia coli direct interaction direct interaction nbn-mre11-2 2 5 unspecified role unspecified role bait bait Region 440-754 Region 440-754 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey Core domain Region 1-411 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P49959:p.Cys146Ala C146A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C146A'. Wed May 02 14:57:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys146ala' Tue Jun 30 20:05:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys146Ala' region Δ87-117 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ87-117'. P49959:p.Arg80Ala R80A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R80A'. Wed May 02 17:16:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg80ala' Tue Jun 30 19:19:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg80Ala' P49959:p.Asp86Leu D86L mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D L This feature has been corrected as a result of our quality control procedures. The original label was 'D86L'. Wed May 02 18:05:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp86leu' Tue Jun 30 18:37:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp86Leu' P49959:p.Asn117Leu N117L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N L This feature has been corrected as a result of our quality control procedures. The original label was 'N117L'. Wed May 02 15:00:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn117leu' Tue Jun 30 18:34:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn117Leu' P49959:p.Pro121Gly P121G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P G This feature has been corrected as a result of our quality control procedures. The original label was 'P121G'. Wed May 02 15:29:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro121gly' Tue Jun 30 17:21:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro121Gly' P49959:p.Ser104Cys S104C mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S C This feature has been corrected as a result of our quality control procedures. The original label was 'S104C'. Wed May 02 17:38:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser104cys' Tue Jun 30 20:18:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser104Cys' P49959:p.Arg202Gly R202G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R G This feature has been corrected as a result of our quality control procedures. The original label was 'R202G'. Wed May 02 18:04:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg202gly' Tue Jun 30 16:26:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg202Gly' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction mre11-16 1 6 unspecified role unspecified role unspecified role unspecified role Core domain Region 1-411 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P49959:p.Cys146Ala C146A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C146A'. Wed May 02 16:54:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys146ala' Tue Jun 30 18:29:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys146Ala' P49959:p.Cys146Arg C146R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C R This feature has been corrected as a result of our quality control procedures. The original label was 'C146R'. Wed May 02 19:56:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys146arg' Tue Jun 30 16:21:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys146Arg' ecolx Escherichia coli direct interaction direct interaction mre11-17 4 6 unspecified role unspecified role unspecified role unspecified role Coredomain Region 1-411 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction