DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218947X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions acis1 Acidianus sp. (strain A1-3) saxs x ray scattering saxs predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218948X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218943X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218941X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218942X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions acis1 Acidianus sp. (strain A1-3) solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218945X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions acis1 Acidianus sp. (strain A1-3) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218944X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. Nature 2011 imex curation Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. Only protein-protein interactions acis1 Acidianus sp. (strain A1-3) comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Smeulders MJ., Barends TR., Pol A., Scherer A., Zandvoort MH., Udvarhelyi A., Khadem AF., Menzel A., Hermans J., Shoeman RL., Wessels HJ., van den Heuvel LP., Russ L., Schlichting I., Jetten MS., Op den Camp HJ. DIP original experiment: DIP-218946X Nature 2011 IMEx Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon. cs2h_acis1 Carbon disulfide hydrolase Carbon disulfide lyase csh protein protein acis1 Acidianus sp. (strain A1-3) MVSEYIDSELKRLEDYALRRVKGIPNNRRLWVLTCMDERVHIEQSLGIQPDDAHIYRNAGGIVTDDAIRSASLTTNFFGTKEIIVVTHTDCGMLRFTGEEVAKYFISKGIKPTEVQLDPLLPAFRISSEEDFIKWFKFYEDLGVKSPDEMALKGVEILRNHPLIPKDVRITGYVYEVETHRLRKPNQIIYNETSKFEHGTIVKE DIP protein G0WXL9 original sequence version: 1 csh-1 6 9 unspecified role unspecified role unspecified role unspecified role acis1 Acidianus sp. (strain A1-3) direct interaction direct interaction csh-2 4 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction csh-3 8 9 unspecified role unspecified role unspecified role unspecified role acis1 Acidianus sp. (strain A1-3) direct interaction direct interaction csh-4 5 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction csh-5 1 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction csh-6 3 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction csh-7 2 9 unspecified role unspecified role unspecified role unspecified role acis1 Acidianus sp. (strain A1-3) direct interaction direct interaction csh-8 7 9 unspecified role unspecified role unspecified role unspecified role acis1 Acidianus sp. (strain A1-3) direct interaction direct interaction