DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218893X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218887X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218890X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218892X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218889X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218891X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 2011 imex curation Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Rasmussen SG., DeVree BT., Zou Y., Kruse AC., Chung KY., Kobilka TS., Thian FS., Chae PS., Pardon E., Calinski D., Mathiesen JM., Shah ST., Lyons JA., Caffrey M., Gellman SH., Steyaert J., Skiniotis G., Weis WI., Sunahara RK., Kobilka BK. DIP original experiment: DIP-218888X Nature 2011 IMEx Crystal structure of the β2 adrenergic receptor-Gs protein complex. gnas2_bovin Guanine nucleotide-binding protein G(s) subunit alpha isoforms short GNAS GNAS1 Adenylate cyclase-stimulating G alpha protein protein protein bovin Bos taurus (Bovine) Bovine MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL DIP protein P04896 original sequence version: 127 DIP protein P04896-1 original sequence version: 164 4D07A734AFF2A6BC gbb1_rat Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Transducin beta chain 1 Gnb1 protein protein rat Rattus norvegicus (Rat) Rat MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN DIP protein P54311 original sequence version: 123 DIP protein P54311 original sequence version: 96 896CBD32D2686598 gbg2_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 GNG2 G gamma-I protein protein bovin Bos taurus (Bovine) Bovine MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL DIP protein P63212 original sequence version: 54 DIP protein P63212 original sequence version: 55 DIP protein P63212 original sequence version: 66 EDB74E4135E7A37A adrb2_human Beta-2 adrenergic receptor Beta-2 adrenoreceptor ADRB2 ADRB2R B2AR protein protein human Homo sapiens Human MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL DIP protein P07550 original sequence version: 180 DIP protein P07550 original sequence version: 144 408C22731C6EDFBE gnb1-gnas-1 2 10 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association adrb2-gnas-1 4 11 unspecified role unspecified role bait bait Region 1-365 Region 1-365 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association gnas-gnb1-1 5 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association adrb2-gnas-2 6 11 unspecified role unspecified role unspecified role unspecified role Region 1-365 Region 1-365 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association gnas-gnb1-2 7 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association adrb2-gnas-3 3 11 unspecified role unspecified role unspecified role unspecified role Region 1-365 Region 1-365 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association adrb2-gnas-4 1 11 unspecified role unspecified role unspecified role unspecified role Region 1-365 Region 1-365 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association