DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218833X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218830X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218832X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218831X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218829X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Hierarchical binding of cofactors to the AAA ATPase p97. Structure (London, England : 1993) 2011 imex curation Hänzelmann P., Buchberger A., Schindelin H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hänzelmann P., Buchberger A., Schindelin H. DIP original experiment: DIP-218828X Structure (London, England : 1993) 2011 IMEx Hierarchical binding of cofactors to the AAA ATPase p97. ufd1_human Ubiquitin recognition factor in ER-associated degradation protein 1 UFD1 UFD1L Ubiquitin fusion degradation protein 1 protein protein human Homo sapiens Human MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP DIP protein Q92890 original sequence version: 91 DIP protein Q92890 original sequence version: 134 FC69860002C042C6 npl4_human Nuclear protein localization protein 4 homolog NPLOC4 KIAA1499 NPL4 protein protein human Homo sapiens Human MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT DIP protein Q8TAT6 original sequence version: 79 6ED6A0145F15C01D q9unn5-1 FAS-associated factor 1 FAF1 UBXD12 UBXN3A CGI-03 UBX domain-containing protein 12 UBX domain-containing protein 3A protein protein human Homo sapiens Human MASNMDREMILADFQACTGIENIDEAITLLEQNNWDLVAAINGVIPQENGILQSEYGGETIPGPAFNPASHPASAPTSSSSSAFRPVMPSRQIVERQPRMLDFRVEYRDRNVDVVLEDTCTVGEIKQILENELQIPVSKMLLKGWKTGDVEDSTVLKSLHLPKNNSLYVLTPDLPPPSSSSHAGALQESLNQNFMLIITHREVQREYNLNFSGSSTIQEVKRNVYDLTSIPVRHQLWEGWPTSATDDSMCLAESGLSYPCHRLTVGRRSSPAQTREQSEEQITDVHMVSDSDGDDFEDATEFGVDDGEVFGMASSALRKSPMMPENAENEGDALLQFTAEFSSRYGDCHPVFFIGSLEAAFQEAFYVKARDRKLLAIYLHHDESVLTNVFCSQMLCAESIVSYLSQNFITWAWDLTKDSNRARFLTMCNRHFGSVVAQTIRTQKTDQFPLFLIIMGKRSSNEVLNVIQGNTTVDELMMRLMAAMEIFTAQQQEDIKDEDEREARENVKREQDEAYRLSLEADRAKREAHEREMAEQFRLEQIRKEQEEEREAIRLSLEQALPPEPKEENAEPVSKLRIRTPSGEFLERRFLASNKLQIVFDFVASKGFPWDEYKLLSTFPRRDVTQLDPNKSLLEVKLFPQETLFLEAKE DIP protein Q9UNN5-1 original sequence version: 104 tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein P55072 original sequence version: 89 DIP protein P55072 original sequence version: 114 DIP protein P55072 original sequence version: 123 DIP protein P55072 original sequence version: 150 DIP protein P55072 original sequence version: 174 DIP protein P55072 original sequence version: 175 DIP protein P55072 original sequence version: 176 DIP protein P55072 original sequence version: 160 DIP protein P55072 original sequence version: 128 DIP protein P55072 original sequence version: 132 501B721D3A77BA8A nsf1c_human NSFL1 cofactor p47 p97 cofactor p47 NSFL1C UBXN2C UBX domain-containing protein 2C protein protein human Homo sapiens Human MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT DIP protein Q9UNZ2 original sequence version: 104 DIP protein Q9UNZ2 original sequence version: 145 DIP protein Q9UNZ2 original sequence version: 112 79364617F940B9F9 faf1-vcp-1 6 10 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-187 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role UBX domain Region 568-650 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction faf1-vcp-2 2 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vcp-faf1-3 3 10 unspecified role unspecified role bait bait Region 1-480 Region 1-480 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey UBX domain Region 568-650 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9UNN5-1:p.Arg579Ala R579A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R579A'. Wed May 02 20:02:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg579ala' Tue Jun 30 20:23:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg579Ala' Q9UNN5-1:p.Phe619Ala F619A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F619A'. Wed May 02 16:24:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe619ala' Tue Jun 30 18:51:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe619Ala' Q9UNN5-1:p.Pro620Asn P620N mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P N This feature has been corrected as a result of our quality control procedures. The original label was 'P620N'. Wed May 02 16:37:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro620asn' Tue Jun 30 20:16:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro620Asn' Q9UNN5-1:p.Arg621Ala R621A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R621A'. Wed May 02 14:53:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg621ala' Tue Jun 30 17:41:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg621Ala' Q9UNN5-1:p.Pro640Asn P640N mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P N This feature has been corrected as a result of our quality control procedures. The original label was 'P640N'. Wed May 02 19:21:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro640asn' Tue Jun 30 19:18:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro640Asn' ecolx Escherichia coli direct interaction direct interaction faf1-nploc4-1 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association nsfl1c-vcp-13 5 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 1-480 Region 1-480 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction vcp-faf1-4 1 10 unspecified role unspecified role bait bait Region 1-480 Region 1-480 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P55072:p.Val38Ala V38A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V38A'. Wed May 02 18:45:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val38ala' Tue Jun 30 18:19:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val38Ala' P55072:p.Phe52Ala F52A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F52A'. Wed May 02 18:59:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe52ala' Tue Jun 30 19:11:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe52Ala' P55072:p.Ile70Ala I70A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I70A'. Wed May 02 15:45:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile70ala' Tue Jun 30 19:55:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile70Ala' P55072:p.Leu72Ala L72A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L72A'. Wed May 02 19:03:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu72ala' Tue Jun 30 18:20:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu72Ala' P55072:p.Tyr110Ala Y110A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y110A'. Wed May 02 20:10:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr110ala' Tue Jun 30 16:31:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr110Ala' P55072:p.Glu141Ala E141A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E141A'. Wed May 02 20:10:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu141ala' Tue Jun 30 19:09:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu141Ala' ecolx Escherichia coli 9 unspecified role unspecified role prey prey UBX domain Region 568-650 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction