DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure and mechanism of the hexameric MecA-ClpC molecular machine. Structure and mechanism of the hexameric MecA-ClpC molecular machine. Nature 2011 imex curation Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219161X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. Structure and mechanism of the hexameric MecA-ClpC molecular machine. Structure and mechanism of the hexameric MecA-ClpC molecular machine. Nature 2011 imex curation Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219159X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. Structure and mechanism of the hexameric MecA-ClpC molecular machine. Structure and mechanism of the hexameric MecA-ClpC molecular machine. Nature 2011 imex curation Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219160X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. meca1_bacsu Adapter protein MecA 1 mecA BSU11520 protein protein bacsu Bacillus subtilis MEIERINEHTVKFYMSYGDIEDRGFDREEIWYNRERSEELFWEVMDEVHEEEEFAVEGPLWIQVQALDKGLEIIVTKAQLSKDGQKLELPIPEDKKQEPASEDLDALLDDFQKEEQAVNQEEKEQKLQFVLRFGDFEDVISLSKLNVNGSKTTLYSFENRYYLYVDFCNMTDEEVENQLSILLEYATESSISIHRLEEYGKLIISEHALETIKKHFAS F6E377D8EA241B7D F6E377D8EA241B7D clpc_bacsu Negative regulator of genetic competence ClpC/MecB clpC mecB BSU00860 protein protein bacsu Bacillus subtilis MMFGRFTERAQKVLALAQEEALRLGHNNIGTEHILLGLVREGEGIAAKALQALGLGSEKIQKEVESLIGRGQEMSQTIHYTPRAKKVIELSMDEARKLGHSYVGTEHILLGLIREGEGVAARVLNNLGVSLNKARQQVLQLLGSNETGSSAAGTNSNANTPTLDSLARDLTAIAKEDSLDPVIGRSKEIQRVIEVLSRRTKNNPVLIGEPGVGKTAIAEGLAQQIINNEVPEILRDKRVMTLDMGTVVAGTKYRGEFEDRLKKVMDEIRQAGNIILFIDELHTLIGAGGAEGAIDASNILKPSLARGELQCIGATTLDEYRKYIEKDAALERRFQPIQVDQPSVDESIQILQGLRDRYEAHHRVSITDDAIEAAVKLSDRYISDRFLPDKAIDLIDEAGSKVRLRSFTTPPNLKELEQKLDEVRKEKDAAVQSQEFEKAASLRDTEQRLREQVEDTKKSWKEKQGQENSEVTVDDIAMVVSSWTGVPVSKIAQTETDKLLNMENILHSRVIGQDEAVVAVAKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESIFGDEESMIRIDMSEYMEKHSTSRLVGSPPGYVGYDEGGQLTEKVRRKPYSVVLLDEIEKAHPDVFNILLQVLEDGRLTDSKGRTVDFRNTILIMTSNVGASELKRNKYVGFNVQDETQNHKDMKDKVMGELKRAFRPEFINRIDEIIVFHSLEKKHLTEIVSLMSDQLTKRLKEQDLSIELTDAAKAKVAEEGVDLEYGARPLRRAIQKHVEDRLSEELLRGNIHKGQHIVLDVEDGEFVVKTTAKTN 9592231886C2E870 mint Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with mecA to negatively regulate comK 9592231886C2E870 clpc-meca-4 2 5 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain D1-M domain Region 150-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 121-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P37958:p.Glu184Arg E184R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E184R'. Wed May 02 18:54:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu184arg' Tue Jun 30 17:30:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu184Arg' ecolx Escherichia coli direct interaction direct interaction clpc-meca-5 1 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 108-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction clpc-meca-6 3 5 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 121-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction