UniProt UniProt http://www.uniprot.org Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Molecular biology of the cell 2011 imex curation Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions mouse-atdc5 mouse ATDC5 chondrocytic cell line Mouse atdc5 ATDC5 chondrogenic cell line confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Accepted 2017-APR-24 AT 08:02 BST AT 08:02 BST by MGT Molecular biology of the cell 2011 imex curation agreed and changed; agreed and changed; 1- for arid1a binding sites: "...Both the ΔC1 and ΔC2 mutants associated with Sox9 (Figure 7B). Although the ΔN2 mutant showed modest binding to Sox9, the ΔN1 mutant did not interact with Sox9 (Figure 7B)...." means 1-293 is sufficient and 150-589 is sufficient.do you think we shoud enter 150-293 as required to bind??? (feel always unsure with these deductions) 2 - sox9 as human -ok, agreed, thought they (or nanother cited ref ) have cloned from pcr... but they might have just changed tags Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Molecular biology of the cell 2011 imex curation Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Accepted 2017-APR-24 AT 08:02 BST AT 08:02 BST by MGT Molecular biology of the cell 2011 imex curation agreed and changed; agreed and changed; 1- for arid1a binding sites: "...Both the ΔC1 and ΔC2 mutants associated with Sox9 (Figure 7B). Although the ΔN2 mutant showed modest binding to Sox9, the ΔN1 mutant did not interact with Sox9 (Figure 7B)...." means 1-293 is sufficient and 150-589 is sufficient.do you think we shoud enter 150-293 as required to bind??? (feel always unsure with these deductions) 2 - sox9 as human -ok, agreed, thought they (or nanother cited ref ) have cloned from pcr... but they might have just changed tags Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Molecular biology of the cell 2011 imex curation Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-bosc_23 Homo sapiens embryonic kidney cell line BOSC-23 bosc_23 Human embryonic kidney cell line BOSC-23 The BOSC-23 cell line are a derivative of the 293T cell line. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Amano K., Hata K., Muramatsu S., Wakabayashi M., Takigawa Y., Ono K., Nakanishi M., Takashima R., Kogo M., Matsuda A., Nishimura R., Yoneda T. Origin of Sox9 according PubMed 18677406 following cited Hata et al, 2008, PubMed 19759178 rikonisi@dent.osaka-u.ac.jp rikonis@dent.osaka-u.ac.jp Accepted 2017-APR-24 AT 08:02 BST AT 08:02 BST by MGT Molecular biology of the cell 2011 imex curation agreed and changed; agreed and changed; 1- for arid1a binding sites: "...Both the ΔC1 and ΔC2 mutants associated with Sox9 (Figure 7B). Although the ΔN2 mutant showed modest binding to Sox9, the ΔN1 mutant did not interact with Sox9 (Figure 7B)...." means 1-293 is sufficient and 150-589 is sufficient.do you think we shoud enter 150-293 as required to bind??? (feel always unsure with these deductions) 2 - sox9 as human -ok, agreed, thought they (or nanother cited ref ) have cloned from pcr... but they might have just changed tags sox9_human Transcription factor SOX-9 SOX9 protein protein human Homo sapiens Human MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP DIP protein P48436 original sequence version: 154 9289CFBB8D6631A2 ari5a_mouse AT-rich interactive domain-containing protein 5A Arid5a protein protein mouse Mus musculus Mouse MAAPPAKGNTEQSEEGDLPQLPVSPKPDDEQSRSQSPTQLQDSPEAGGEQEEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLKGEDDKPLPPTKPRKQYKMAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRDSTEQLGPVSGPSPPLTGASSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGARSPNKDIQDSPQNLRGPAENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQHPRDFFSGLKDRVLLGPPGKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPTERPGAPSPHPSSPGLGSKRGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPTPPEAYRGTMLRCPLNFTGSADPLKGQASLPFSPLVIPAFPAHLLATTGSSPMAASLMHFPPTPYDAVLRNRLGPASSAWHMPPVTTYAAPHFFHLNTKL D4C00C2F1DEBDCC3 arid5a-sox9-1 3 4 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2A arid5a-sox9-2 1 4 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection dsred fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind range range range range region venus venus fluorescent protein tag venus undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range colocalization colocalization 2B, 7C sox9-arid5a-1 2 5 unspecified role unspecified role prey prey cell lysate cell lysate region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region required to bind necessary binding region required to bind range range range range human-bosc_23 Homo sapiens embryonic kidney cell line BOSC-23 bosc_23 Human embryonic kidney cell line BOSC-23 The BOSC-23 cell line are a derivative of the 293T cell line. 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 7B