DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 2011 imex curation Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219170X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 2011 imex curation Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219168X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 2011 imex curation Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219169X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 2011 imex curation Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219167X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. q9w4m7_drome tyf CG485 Dmel\CG4857 EG:EG0007.12 EG:EG0007.4 TYF Dmel_CG4857 CG4857 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNNEQTPSSSDTPADRVASGGAAAKLPTIIPDTPIVSIAAGTMSESTTTTTVTKTTPTPFPQEQLMTMMSDHVAKADAQLDEACRNQGAKDEDSQGAAPVAANHVATSGSSSRGNDPKKSSSPSNSHSSGNHAATADAAKGSAAEAAAGDTSFSQVFAMLKDSPSAELSRPNRRVLQYGATAKGKTQDDQNVGKRQKKKKTKYDTWEDSDFNDLDDASLKKLLEEAYWYRNPGDRKNKSERFLQMLKKAEYDEEISYRAIKSCLTINPSALTSSAATGSSGGSSSSAGHNSSYNSTNHRSTDPGQRHKQGGSLQDLVEAAHRSELATTSAAAAAAATAAATQRFNCDYQLSGRSNRRQQQQQQQHNQNATSSASASKKIKNLNVSGRQREGGSLPSSVNFEPASSAMEAKQNKQQAQPSTEAAGGSFSGSVGNKHHHRSSTGSCSSLITQSRDTEANNQFDLDMDDATGVGASAVVGASGDQDDNMLEQLDPQNPSMKFLIDALNDTPLEARYTGAGKKFLIDDRLHFSVLELSARRKLQQQAQYLAALTGQFGLGLEEEKRKVEAGYVSLNDNYTACSSVYGGGAGSPAAVEGSKPSTSSSAVSGGSNCGASSDALSSDLRPAKIGRMVPAAAAVAAAPMGGKTTTRQLDENGNALGDGFGGSAASGSSGASGASAAASSSNGNGNANQFTALITPTVGSSVPTSTVAHRQNSVSTLLSTGTNTTTTAMAAAAVAASYSQLQQAPVGGAAGTVSGIGIGMQQPTKQKTKKKSQQERNTTTVDVESVAGYRGNDPVEQLVKYIENDVNGGGNSAAGQRKKERKKQNKLKKSNSLEELRTCSKMEVDDLKRQSATTEMMRHKKGTNNASSGGSSSTASGSSNSGKHNSASVADINKNCNKEQQQQSTPTVQVRNSNNPGTQQRKGERRSWGTEELQYLGDHQEISAAWSELETVGQKELPLALPALARMSELDALNTVLSETAEFHVVTKKKKPKKQRAVTMDDAAVAAAAVTGGNLQRMQQITKSASSNIMSQRMHYYTPYNSNNGGGSVNYKQQQQQSQQYQEHYQAPQGQHHHHHHHHHHHHHHHSGSAVSNQVDSSRRKSTSSMPPSEKSDSSDLDSVHSLPIQTGKKKSLGGGNNKQRGAPSASQRQAKQNNNAAAPISYADIARNKQEALNNATASDTELELGDKMQIKSGGKSKSRPDFPELPGAVTAVAASGGAANQATVVSSQNTPPSSSSSISYSQSLNATPPSSSSDAESTNSPELLAQVQIPAYSMITPTLTASMISSVSSSVASTSSTSCSPPALQKSKSVEHDTSYSCNSSNLDQQYPALEKTVKRHSTNNVSVAVAASTSASASSSLYNFAAAAKQQLTENTHSAVSPPAAVTTTSTAVAASAPAPTTVTVPAAVTVPCYSSSSTHGSTQTSTSTASSAIAALPSGKAKAKPKELSPSSSCSKKPTKPSQDVPSISLSISTTPRATTSTSTTTTQKTTAATQTEGAKKLIGGIHKLPSSSCIAKGAGAVLEATAGRRAVIILNDDREAGRSNNEFIFGDFNEDELKLFDDNLDDEEEGQHKQSSNKKQQTEGESDVTQDDTEDVDQDLDKELECLGRRPEKKQERQQDISASSDQHLNDSGAASDAVNSSASLDMLSISAEAAQSSPNAGATATSSSVASPINSSTPSGASSASASTSTSSSSVSISSSSGGNGAACLASVSGMLGGVANQSGDSGIYAAANTSIKEHVNTFLSKASSSEETLPSPNSISMQQLETCNDIEAAIIAAARAAAAARSTSCSRSNSQEQDALQPQVKTKATPNPEAKMVLPVFVTYNNNNNDDDDDEEDDESLQELSFMADLKADAATEDIAMQPPPPSCPAMMTNTELIVDYIAKTWNAIANSKYVTYYNEQEQEQET DIP protein Q9W4M7 original sequence version: 58 DIP protein Q9W4M7 original sequence version: 100 78EBE281C9EFE93F if4e_drome Eukaryotic translation initiation factor 4E1 eIF4E1 Eif4e eIF-4E eIF-4F 25 kDa subunit mRNA cap-binding protein CG4035 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSDFHRMKNFANPKSMFKTSAPSTEQGRPEPPTSAAAPAEAKDVKPKEDPQETGEPAGNTATTTAPAGDDAVRTEHLYKHPLMNVWTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKDTMVKQGSNVKSIYTL DIP protein P48598 original sequence version: 96 B844B2DD5738758E pabp_drome Polyadenylate-binding protein pAbp CG5119 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN DIP protein P21187 original sequence version: 105 DIP protein P21187 original sequence version: 146 A76B204ED206392E tyf-pabp-1 4 7 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association eif_4e-pabp-1 3 6 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association pabp-tyf-1 1 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey N-terminal domain Region 1-1167 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction eif_4e-pabp-2 2 6 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly association association