DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Nature structural & molecular biology 2011 imex curation Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218500X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Nature structural & molecular biology 2011 imex curation Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218498X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Nature structural & molecular biology 2011 imex curation Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218496X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Nature structural & molecular biology 2011 imex curation Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218497X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Structural basis for engagement by complement factor H of C3b on a self surface. Nature structural & molecular biology 2011 imex curation Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218499X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. co3_human Complement C3 C3 CPAMD1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 protein protein human Homo sapiens Human MGPTSGPSLLLLLLTHLPLALGSPMYSIITPNILRLESEETMVLEAHDAQGDVPVTVTVHDFPGKKLVLSSEKTVLTPATNHMGNVTFTIPANREFKSEKGRNKFVTVQATFGTQVVEKVVLVSLQSGYLFIQTDKTIYTPGSTVLYRIFTVNHKLLPVGRTVMVNIENPEGIPVKQDSLSSQNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVFSTEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEVTITARFLYGKKVEGTAFVIFGIQDGEQRISLPESLKRIPIEDGSGEVVLSRKVLLDGVQNPRAEDLVGKSLYVSATVILHSGSDMVQAERSGIPIVTSPYQIHFTKTPKYFKPGMPFDLMVFVTNPDGSPAYRVPVAVQGEDTVQSLTQGDGVAKLSINTHPSQKPLSITVRTKKQELSEAEQATRTMQALPYSTVGNSNNYLHLSVLRTELRPGETLNVNFLLRMDRAHEAKIRYYTYLIMNKGRLLKAGRQVREPGQDLVVLPLSITTDFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCVGSLVVKSGQSEDRQPVPGQQMTLKIEGDHGARVVLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGSGKDYAGVFSDAGLTFTSSSGQQTAQRAELQCPQPAARRRRSVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARASHLGLARSNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN DIP protein P01024 original sequence version: 140 DIP protein P01024 original sequence version: 148 30C2832A9E75FFC4 cfah_human Complement factor H CFH HF HF1 HF2 H factor 1 protein protein human Homo sapiens Human MRLLAKIICLMLWAICVAEDCNELPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCIRVKTCSKSSIDIENGFISESQYTYALKEKAKYQCKLGYVTADGETSGSITCGKDGWSAQPTCIKSCDIPVFMNARTKNDFTWFKLNDTLDYECHDGYESNTGSTTGSIVCGYNGWSDLPICYERECELPKIDVHLVPDRKKDQYKVGEVLKFSCKPGFTIVGPNSVQCYHFGLSPDLPICKEQVQSCGPPPELLNGNVKEKTKEEYGHSEVVEYYCNPRFLMKGPNKIQCVDGEWTTLPVCIVEESTCGDIPELEHGWAQLSSPPYYYGDSVEFNCSESFTMIGHRSITCIHGVWTQLPQCVAIDKLKKCKSSNLIILEEHLKNKKEFDHNSNIRYRCRGKEGWIHTVCINGRWDPEVNCSMAQIQLCPPPPQIPNSHNMTTTLNYRDGEKVSVLCQENYLIQEGEEITCKDGRWQSIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR DIP protein P08603 original sequence version: 161 DIP protein P08603-1 original sequence version: 149 3C26D62A2BF9BFEE c3-cfh-5 2 7 unspecified role unspecified role prey prey CCP19-20 domain Region 1107-1231 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P08603:p.Asp1119Gly D1119G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D G This feature has been corrected as a result of our quality control procedures. The original label was 'D1119G'. Wed May 02 15:06:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1119gly' Tue Jun 30 19:16:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1119Gly' P08603:p.Arg1182Ser R1182S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R S This feature has been corrected as a result of our quality control procedures. The original label was 'R1182S'. Wed May 02 15:09:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1182ser' Tue Jun 30 19:30:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1182Ser' P08603:p.Arg1203Ser R1203S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R S This feature has been corrected as a result of our quality control procedures. The original label was 'R1203S'. Wed May 02 20:03:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1203ser' Tue Jun 30 18:03:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1203Ser' P08603:p.Arg1215Gln R1215Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q This feature has been corrected as a result of our quality control procedures. The original label was 'R1215Q'. Wed May 02 15:12:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1215gln' Tue Jun 30 16:40:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1215Gln' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role bait bait C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction c3-cfh-6 3 7 unspecified role unspecified role unspecified role unspecified role CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role unspecified role unspecified role C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cfh-c3-1 1 7 unspecified role unspecified role bait bait CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role prey prey C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction c3-cfh-7 4 7 unspecified role unspecified role unspecified role unspecified role CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role unspecified role unspecified role C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction c3-cfh-8 5 7 unspecified role unspecified role prey prey CCP19-20 domain Region 1107-1231 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role bait bait C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P01024:p.[Glu1110Ala;Asp1115Ala] E1110A/D1115A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1110A/D1115A'. Wed May 02 17:06:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1110ala,asp1115ala' Tue Jun 30 18:59:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu1110Ala;Asp1115Ala]' P01024:p.Glu1110Ala E1110A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1110A'. Wed May 02 16:13:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1110ala' Tue Jun 30 18:08:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1110Ala' P01024:p.Asp1115Ala D1115A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D1115A'. Wed May 02 15:42:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1115ala' Tue Jun 30 19:59:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1115Ala' P01024:p.Glu1153Ala E1153A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1153A'. Wed May 02 16:36:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1153ala' Tue Jun 30 19:26:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1153Ala' P01024:p.Ile1157Ala I1157A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I1157A'. Wed May 02 16:33:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile1157ala' Tue Jun 30 19:36:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1157Ala' ecolx Escherichia coli direct interaction direct interaction