DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS biology 2011 imex curation Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions psepf Pseudomonas fluorescens anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216749X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS biology 2011 imex curation Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions psepf Pseudomonas fluorescens pull down pull down predetermined predetermined participant predetermined Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216751X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS biology 2011 imex curation Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216748X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS biology 2011 imex curation Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions psepf Pseudomonas fluorescens pull down pull down predetermined predetermined participant predetermined Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216750X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. q3kk29_psepf lapA Pfl01_0133 protein protein psepf Pseudomonas fluorescens MTPGESSMSSVVAIVKSIVGQVFVVSPEGVRRVLVEGDRLFAGDQVDTGVSGAVSLELADGRTIDLGRDTQWSADTPDSSTDLAEATAQAAPSVEELQQAIAAGVDPTTALESTAAGPSAAGTGGAAGGGHSFVMLDATAGRVDPTIGFPTAGINAAGQAAQNITGGQNTDTTANALRESTLSLSATPSITEAGGVLVYTATLTQAPLTDLTITLSNGAVIVIPAGSTTGTANVPLAPNDTVYNDPTQIDVTVTGTTGGSGITVTPPTTPATTQVTDTIDTTTVTLTAGPSVTEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGTVNVPTAPNDVYNNGSTVTTTITGATGGNFENLVPNPTPAVTTITDSIDTTGLTLTATGTVVEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGSVNVPTAPNDVYNNGGTVSTTITGATGGNFENLVPNTTPATTTVTDSIDTTNLSLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKIEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTSTVKLTADSSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTVTTTAPNDALAGNAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADITNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDVLTGHAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADTTNLTLSATGTVAEGGSIVYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTTTVKLTATESAVEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTSVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTRVTDTVDTTNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDALNGHTPLSNSITGVTGGNYENLVADKTPVSTTVTDTVDTTDLTLTATDSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAVEGGTVSYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITNVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTAPAVTEVTDTIDTSTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITDVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATPEVNEGGQITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVETTISTTTGGNFENLVTSTAPAVTTVNDTIDTSTVSLTATANVAEGETVVYTATVTAPVTGSPVVVTLSNGQTITIAVGETTGTVNFVAPNSPLAGGSSLSVTIDKATGGNYENLAVDGKSADTSVSDTVDTTNLNLTATDSVAEGGSITYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGGFENLVPSTVPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTIGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTASETAAEGGTVTYTATVGAPVTGSPVTVTLANGQQITIEVGKTTGTVTTTAPNDVLNDHAPLTNAITDVSGGNYENLVADKTPVSTTITDTIDTTNLSLTATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGTVEATIKSATGGNFENLVTSTTPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLSNGQSITIEVGKTTGTVTTTAPNDALTGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATNSVAEGGSIIYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVPSTVPAVTQVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGTPVTVTLSNGAVIIIEAGKTTGTVTVAAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLTGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGNVVYTATVGAPVTGSPVTVTLSNGQSITIEVGKTSGTVTTTAPNDVLNGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATNSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVIVNLANGQSITIEVGKTTGTVTTIAPNDALTGQAPLTNSITNVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATIKSATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATTTAAEGGTVTYTATVGAPVTGSPVTVNLANGQSITIEVGKTTGTVTTTAPNDALTGHTPLTNSITNVTGGNYENLVADKTPVSTTVTDTIDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIDAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVSITGSTSVTEGQTASYTVSLTHPAQTEVTLKIVYSGTAADGSDFTGVYTVKIPAGASSAQFNVATIDDKITEGTENFVVKIDSATGGNFENLAVSSTNGSVSTSIIDNDAPPVIDLDANNSSGATGADYKVTFTEGTTGPGVSIADTDIKITDPDSTMLTGATIVLTNRQDGDALNLGNSVNGITINANSTNGTVTLTLSGNATLADYMQAIKNISFTNSSEDPSTVPRIITVTVTDGGNYSNTATTTVNVVAINDAPVAAPVNVTGTEDTPLILGWSTFGVSDVDSPASSLGVKITQLPGEGKLQYLDGSTWKDVANNQTFSKADIDAGKLRFVPDANESGVNGYGGTGVGNNQADYAQIKFQPTDGQLLGNTGTIKIDITPVADAPTLSVADNSVKSTGLIKEVWTGLSGLGTDGSGANSTTLKNVIDGAGKPNTSGTVNNVQSDGSVTAGTASKTSGLIYLEAGKTYTFSGTGDDSLLVTIGGKSVASTTWGAGGNLNGSFTPTTSGYYTLDIYHHNQSGPGSYDVNLSVNGSTPIDLSSAGVPIYTGVQDLVNAGVTVSDLHGSNGEGYYDGYKLNTGAEGTTVKLSSISTALTDTDGSETLSIKISGAPVGSVLSDGAGHSFTVTATSGDANVTGWNLGTLTVTPPTYYNGQFNLTVTSTSTEQVGGSASTTATIPVTVVPAVYNSIVATSADDTVTGTDGNDIMVADIGGLTVVPGTNYNIAFMVDSSGSMSASSINAAKDSLTSVFNTLKQSLGGSNSGTVNIFLVDFDTQVNKSVSVNLNDPNALTLLKAVLDSMASGGGTNYEDVFKATANFFQSAEAVANTGAKNLTYFITDGQPTYYQSNEQTNPTLYGNVKLDDVVKTSNYKLGSSFATYIDSTHYLSISTSGEVVLQTLKNNNWSWNSLGTIHAQGDGTYEISSLAGSGNSTSSNTTDNSAAGFALLSGLSNVEAIGLNNGVSLNDLKPYDSDKTPQTNIDPKDLANSIIGHTEATMPGNDTVSGGDGNDILFGDLVSFNGIAGEGYQAMQAFVAKETGVDVSKVTTSNVHQYITEHYQAFDVSGAHDGNDTLLGGAGNDILFGSGGNDLLDGGKGNDILLGGTGNDTLIGGQGNDILIGGSGADTFVWKAGDTGNDVIKDFKASEGDRIDLRDLLQGETGSTIDNFLKITTVDGVSSLQVSSGGKFNSGDAAAATPDVTIKLEGNNWSSANIHNLIAGSDPTIKVDHNNS DIP protein Q3KK29 original sequence version: 49 FBCD98C90EEA1FDE q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN DIP protein Q3KK32 original sequence version: 39 438AE5C5244F9A51 q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG DIP protein Q3KK31 original sequence version: 42 0EC47D6D9BF3C233 lapa-lapf-1 3 5 enzyme target enzyme target substrate substrate unspecified role unspecified role N-terminal domain Region 8-242 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 6 enzyme enzyme unspecified role unspecified role Q3KK32:p.Cys135Ala C135A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C135A'. Wed May 02 19:26:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys135ala' Tue Jun 30 17:00:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys135Ala' ecolx Escherichia coli protein cleavage protein cleavage lapd-lapf-1 4 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range psepf Pseudomonas fluorescens 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range psepf Pseudomonas fluorescens physical association physical association lapf-lapd-1 1 7 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range psepf Pseudomonas fluorescens 6 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range psepf Pseudomonas fluorescens physical association physical association lapd-lapf-2 2 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q3KK31:p.Glu617Ala E617A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E617A'. Wed May 02 16:59:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu617ala' Tue Jun 30 19:55:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu617Ala' Q3KK31:p.Leu152Pro L152P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P This feature has been corrected as a result of our quality control procedures. The original label was 'L152P'. Wed May 02 17:38:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu152pro' Tue Jun 30 17:25:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu152Pro' psepf Pseudomonas fluorescens 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range psepf Pseudomonas fluorescens physical association physical association