DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Structure (London, England : 1993) 2011 imex curation Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218775X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Structure (London, England : 1993) 2011 imex curation Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218776X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. Structure (London, England : 1993) 2011 imex curation Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Boettcher AJ., Wu J., Kim C., Yang J., Bruystens J., Cheung N., Pennypacker JK., Blumenthal DA., Kornev AP., Taylor SS. DIP original experiment: DIP-218777X Structure (London, England : 1993) 2011 IMEx Realizing the allosteric potential of the tetrameric protein kinase A RIα holoenzyme. kap0_bovin cAMP-dependent protein kinase type I-alpha regulatory subunit PRKAR1A protein protein bovin Bos taurus (Bovine) Bovine MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV DIP protein P00514 original sequence version: 101 DIP protein P00514 original sequence version: 111 DIP protein P00514 original sequence version: 133 DIP protein P00514 original sequence version: 149 B086A291809422F4 kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P05132 original sequence version: 185 DIP protein P05132 original sequence version: 186 DIP protein P05132-1 original sequence version: 145 DIP protein P05132-1 original sequence version: 193 DIP protein P05132-1 original sequence version: 199 DIP protein P05132 original sequence version: 163 DIP protein P05132 original sequence version: 192 DIP protein P05132-1 original sequence version: 197 DIP protein P05132-1 original sequence version: 149 02F85D66EB21A1FA prkaca-prkar1a-6 1 4 unspecified role unspecified role unspecified role unspecified role Region 74-245 Region 74-245 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction prkaca-prkar1a-7 2 4 unspecified role unspecified role unspecified role unspecified role Region 74-245 Region 74-245 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction prkaca-prkar1a-8 3 4 unspecified role unspecified role unspecified role unspecified role Region 2-260 Region 2-260 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction