DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253404X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253406X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253407X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253401X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253410X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253405X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253409X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253403X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253400X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253408X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Jennings-Antipov LD., Song L., Collier RJ. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Jennings-Antipov LD., Song L., Collier RJ. DIP original experiment: DIP-253402X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Interactions of anthrax lethal factor with protective antigen defined by site-directed spin labeling. pag_bacan Protective antigen PA-83 Anthrax toxins translocating protein pagA pag pXO1-110 BXA0164 GBAA_pXO1_0164 protein protein bacan Bacillus anthracis MKKRKVLIPLMALSTILVSSTGNLEVIQAEVKQENRLLNESESSSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIPSSELENIPSENQYFQSAIWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQRENPTEKGLDFKLYWTDSQNKKEVISSDNLQLPELKQKSSNSRKKRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVAAYPIVHVDMENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVNTGTAPIYNVLPTTSLVLGKNQTLATIKAKENQLSQILAPNNYYPSKNLAPIALNAQDDFSSTPITMNYNQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNATNIYTVLDKIKLNAKMNILIRDKRFHYDRNNIAVGADESVVKEAHREVINSSTEGLLLNIDKDIRKILSGYIVEIEDTEGLKEVINDRYDMLNISSLRQDGKTFIDFKKYNDKLPLYISNPNYKVNVYAVTKENTIINPSENGDTSTNGIKKILIFSKKGYEIG DIP protein P13423 original sequence version: 106 DIP protein P13423 original sequence version: 91 DIP protein P13423 original sequence version: 93 DIP protein P13423 original sequence version: 110 DIP protein P13423 original sequence version: 124 DIP protein P13423 original sequence version: 150 3AE1EFBF48FAA03F lef_bacan Lethal factor Anthrax lethal toxin endopeptidase component lef pXO1-107 BXA0172 GBAA_pXO1_0172 protein protein bacan Bacillus anthracis MNIKKEFIKVISMSCLVTAITLSGPVFIPLVQGAGGHGDVGMHVKEKEKNKDENKRKDEERNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGGKIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDYVENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLEQNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLARYEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDFLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS DIP protein P15917 original sequence version: 109 DIP protein P15917 original sequence version: 138 DIP protein P15917 original sequence version: 93 DIP protein P15917 original sequence version: 98 DIP protein P15917 original sequence version: 112 DIP protein P15917 original sequence version: 125 2076B4D7277317EE paga-12 9 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction lef-paga-16 2 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain P63 domain Region 197-764 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lef-paga-17 8 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role P13423:p.Phe456Ser F456S/H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 16:19:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456ser' Tue Jun 30 19:59:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456Ser' P13423:p.Phe456His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F H Wed May 02 17:39:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456his' Tue Jun 30 17:32:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456His' ecolx Escherichia coli direct interaction direct interaction lef-paga-18 4 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role P13423:p.[Arg207Ala;Lys243Glu] R207A/K243E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R207A/K243E'. Wed May 02 18:27:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg207ala,lys243glu' Tue Jun 30 19:21:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg207Ala;Lys243Glu]' ecolx Escherichia coli direct interaction direct interaction lef-paga-19 6 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain P63 domain Region 197-764 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lef-paga-20 5 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain P63 domain Region 197-764 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lef-paga-21 10 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction lef-paga-22 3 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction lef-paga-23 11 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role P13423:p.Phe456Ser F456S/H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 14:38:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456ser' Tue Jun 30 17:57:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456Ser' P13423:p.Phe456His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F H Wed May 02 18:42:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456his' Tue Jun 30 17:26:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456His' ecolx Escherichia coli direct interaction direct interaction lef-paga-24 1 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role P13423:p.Phe456Ser F456S/H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 20:27:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456ser' Tue Jun 30 15:38:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456Ser' P13423:p.Phe456His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F H Wed May 02 20:05:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe456his' Tue Jun 30 19:05:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe456His' ecolx Escherichia coli direct interaction direct interaction lef-paga-25 7 13 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 34-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction