DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249109X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249107X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249106X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249105X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249108X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. axin1_human Axin-1 Axis inhibition protein 1 AXIN1 AXIN protein protein human Homo sapiens Human MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD DIP protein O15169 original sequence version: 118 DIP protein O15169 original sequence version: 125 DIP protein O15169-1 original sequence version: 140 DIP protein O15169 original sequence version: 132 10779173F5092F3F dvl2_human Segment polarity protein dishevelled homolog DVL-2 DSH homolog 2 DVL2 protein protein human Homo sapiens Human MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein O14641 original sequence version: 150 DIP protein O14641 original sequence version: 113 DIP protein O14641 original sequence version: 124 DIP protein O14641 original sequence version: 154 DIP protein O14641 original sequence version: 159 4BAD95B6C3FE531B axin1-28 2 6 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'I794A/R797D'. Wed May 02 20:28:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile794ala,arg797asp' Tue Jun 30 18:28:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile794Ala;Arg797Asp]' ecolx Escherichia coli direct interaction direct interaction axin1-2 4 6 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'I794A/R797D'. Wed May 02 16:13:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile794ala,arg797asp' Tue Jun 30 18:12:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile794Ala;Arg797Asp]' O15169:p.Tyr796Asp Y796D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y796D'. Wed May 02 14:37:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr796asp' Tue Jun 30 16:31:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr796Asp' O15169:p.[Lys825Ala;Glu838Leu] K825A/E838L mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E L certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K825A/E838L'. Wed May 02 19:21:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys825ala,glu838leu' Tue Jun 30 19:02:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys825Ala;Glu838Leu]' ecolx Escherichia coli direct interaction direct interaction axin1-dvl2-1 1 7 unspecified role unspecified role unspecified role unspecified role Dix domain Region 1-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14641:p.Tyr27Asp Y27D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D'. Wed May 02 20:28:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 18:15:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation mutation certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I794A/R797D'. Wed May 02 16:46:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile794ala,arg797asp' Tue Jun 30 19:18:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile794Ala;Arg797Asp]' ecolx Escherichia coli direct interaction direct interaction axin1-dvl2-2 5 7 unspecified role unspecified role unspecified role unspecified role Dix domain Region 1-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14641:p.[Val67Ala;Lys68Ala] V67A/K68A mutation mutation certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A/K68A'. Wed May 02 15:31:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 17:06:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation mutation certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I794A/R797D'. Wed May 02 19:36:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile794ala,arg797asp' Tue Jun 30 17:02:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile794Ala;Arg797Asp]' ecolx Escherichia coli direct interaction direct interaction axin1-3 3 6 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I794A/R797D'. Wed May 02 15:20:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile794ala,arg797asp' Tue Jun 30 20:13:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile794Ala;Arg797Asp]' O15169:p.Tyr796Asp Y796D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y796D'. Wed May 02 18:02:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr796asp' Tue Jun 30 17:09:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr796Asp' O15169:p.[Lys825Ala;Glu838Leu] K825A/E838L mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E L certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K825A/E838L'. Wed May 02 14:37:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys825ala,glu838leu' Tue Jun 30 20:18:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys825Ala;Glu838Leu]' ecolx Escherichia coli direct interaction direct interaction