DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Byrnes LJ., Sondermann H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249652X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Byrnes LJ., Sondermann H. Only protein-protein interactions in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249655X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Byrnes LJ., Sondermann H. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249654X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Proceedings of the National Academy of Sciences of the United States of America 2011 imex curation Byrnes LJ., Sondermann H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249653X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. atla1_human Atlastin-1 ATL1 GBP3 SPG3A Spastic paraplegia 3 protein A Guanine nucleotide-binding protein 3 GTP-binding protein 3 Brain-specific GTP-binding protein protein protein human Homo sapiens Human MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQLESEIDELYIQYIKHNDSKNIFHAARTPATLFVVIFITYVIAGVTGFIGLDIIASLCNMIMGLTLITLCTWAYIRYSGEYRELGAVIDQVAAALWDQGSTNEALYKLYSAAATHRHLYHQAFPTPKSESTEQSEKKKM DIP protein Q8WXF7 original sequence version: 118 DIP protein Q8WXF7 original sequence version: 95 68A33C39DD43504C atl1-1 3 5 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction atl1-4 1 5 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction atl1-5 2 5 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8WXF7:p.Arg77Glu R77E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R77E'. Wed May 02 14:48:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg77glu' Tue Jun 30 19:55:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg77Glu' Q8WXF7:p.Gln191Arg Q191R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q R This feature has been corrected as a result of our quality control procedures. The original label was 'Q191R'. Wed May 02 17:30:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln191arg' Tue Jun 30 17:47:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln191Arg' Q8WXF7:p.Arg217Gln R217Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R Q This feature has been corrected as a result of our quality control procedures. The original label was 'R217Q'. Wed May 02 15:24:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg217gln' Tue Jun 30 19:01:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg217Gln' Q8WXF7:p.Tyr336His Y336H mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y H This feature has been corrected as a result of our quality control procedures. The original label was 'Y336H'. Wed May 02 20:31:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr336his' Tue Jun 30 17:16:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr336His' Q8WXF7:p.Met408Thr M408T/V mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M T Wed May 02 15:08:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met408thr' Tue Jun 30 19:51:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met408Thr' Q8WXF7:p.Met408Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M V Wed May 02 15:05:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met408val' Tue Jun 30 20:22:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met408Val' ecolx Escherichia coli direct interaction direct interaction atl1-6 4 5 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8WXF7:p.Arg77Glu R77E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R77E'. Wed May 02 17:47:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg77glu' Tue Jun 30 15:44:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg77Glu' Q8WXF7:p.Gln191Arg Q191R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q R This feature has been corrected as a result of our quality control procedures. The original label was 'Q191R'. Wed May 02 14:57:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln191arg' Tue Jun 30 19:59:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln191Arg' Q8WXF7:p.Arg217Gln R217Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R Q This feature has been corrected as a result of our quality control procedures. The original label was 'R217Q'. Wed May 02 19:57:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg217gln' Tue Jun 30 17:29:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg217Gln' Q8WXF7:p.Tyr336His Y336H mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y H This feature has been corrected as a result of our quality control procedures. The original label was 'Y336H'. Wed May 02 16:59:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr336his' Tue Jun 30 20:22:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr336His' Q8WXF7:p.Met408Thr M408T/V mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M T Wed May 02 14:30:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met408thr' Tue Jun 30 18:32:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met408Thr' Q8WXF7:p.Met408Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M V Wed May 02 15:37:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met408val' Tue Jun 30 20:02:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met408Val' ecolx Escherichia coli direct interaction direct interaction