DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254409X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254420X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254415X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254412X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254423X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254414X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254410X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254411X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254413X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254417X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254421X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254418X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254419X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254416X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. TRIM24 links a non-canonical histone signature to breast cancer. Nature 2010 imex curation Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254422X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. tif1a_human Transcription intermediary factor 1-alpha TRIM24 RNF82 TIF1 TIF1A Tripartite motif-containing protein 24 RING finger protein 82 E3 ubiquitin-protein ligase TRIM24 RING-type E3 ubiquitin transferase TIF1-alpha protein protein human Homo sapiens Human MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK DIP protein O15164 original sequence version: 133 DIP protein O15164-1 original sequence version: 118 DIP protein O15164 original sequence version: 118 DIP protein O15164 original sequence version: 136 DIP protein O15164 original sequence version: 148 D341E8022AACC67E h4(127) Histone 4 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGRGKGGKGLGKGGAKRHRKVLRDNIQ ing1_human Inhibitor of growth protein 1 ING1 protein protein human Homo sapiens Human MSFVECPYHSPAERLVAEADEGGPSAITGMGLCFRCLLFSFSGRSGVEGGRVDLNVFGSLGLQPWIGSSRCWGGPCSSALRCGWFSSWPPPSKSAIPIGGGSRGAGRVSRWPPPHWLEAWRVSPLPLSPLSPATFGRGFIAVAVIPGLWARGRGCSSDRLPRPAGPARRQFQAASLLTRGWGRAWPWKQILKELDECYERFSRETDGAQKRRMLHCVQRALIRSQELGDEKIQIVSQMVELVENRTRQVDSHVELFEAQQELGDTAGNSGKAGADRPKGEAAAQADKPNSKRSRRQRNNENRENASSNHDHDDGASGTPKEKKAKTSKKKKRSKAKAEREASPADLPIDPNEPTYCLCNQVSYGEMIGCDNDECPIEWFHFSCVGLNHKPKGKWYCPKCRGENEKTMDKALEKSKKERAYNR DIP protein Q9UK53 original sequence version: 112 DIP protein Q9UK53-1 original sequence version: 86 03D6AEEAA6E39090 pf21a_human PHD finger protein 21A BRAF35-HDAC complex protein BHC80 BHC80a PHF21A BHC80 KIAA1696 BM-006 protein protein human Homo sapiens Human MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPDKFQIQPLPQSENKLQTAQQQPLQQLQQQQQYHHHHAQQSAAASPNLTASQKTVTTASMITTKTLPLVLKAATATMPASVVGQRPTIAMVTAINSQKAVLSTDVQNTPVNLQTSSKVTGPGAEAVQIVAKNTVTLVQATPPQPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLAGPQTVQLSKPSLEKQTVKSHTETDEKQTESRTITPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGTRKRGRPPKYNAVLGFGALTPTSPQSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSISKCMEMKNTILARQKEMHSSLEKVKQLIRLIHGIDLSKPVDSEATVGAISNGPDCTPPANAATSTPAPSPSSQSCTANCNQGEETK DIP protein Q96BD5 original sequence version: 104 DIP protein Q96BD5 original sequence version: 80 DIP protein Q96BD5 original sequence version: 130 C7AF87EB2984A796 h3(133) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLATKAARKSAPATGYK esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 h3(133)-trim24-1 1 20 unspecified role unspecified role unspecified role unspecified role Region 1-10 Region 1-10 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(133)-trim24-2 13 20 unspecified role unspecified role unspecified role unspecified role Region 13-32 Region 13-32 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role Bromo domain Region 896-1016 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction trim24-esr1-1 5 21 unspecified role unspecified role prey prey human Homo sapiens Human 16 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association h3(133)-trim24-3 9 20 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 16 unspecified role unspecified role prey prey PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction h3(133)-ing1-1 14 18 unspecified role unspecified role prey prey PHD domain region 353-402 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 20 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal K4me1 K4me1 n6me1lys N6-methyl-L-lysine (2S)-2-amino-6-methylaminohexanoic acid MOD_RES N6-methyllysine N(zeta)-methyllysine N6-methyl-L-lysine N6-methylated L-lysine epsilon-methyllysine Methyl Methylation N-methyl Lysyl N6Me1Lys certain certain sequence position certain certain certain sequence position certain K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain K9me2 K9me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction h3(133)-trim24-4 7 20 unspecified role unspecified role unspecified role unspecified role Region 13-32 Region 13-32 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(133)-trim24-5 6 20 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 16 unspecified role unspecified role prey prey PHD domain Region 824-886 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15164:p.Cys840Trp C840W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C W This feature has been corrected as a result of our quality control procedures. The original label was 'C840W'. Wed May 02 18:31:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys840trp' Tue Jun 30 16:26:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys840Trp' ecolx Escherichia coli direct interaction direct interaction trim24-esr1-2 15 21 unspecified role unspecified role prey prey ecolx Escherichia coli 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association h3(133)-trim24-6 10 20 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 13-32 Region 13-32 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O15164:p.Asp827Ala D827A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D827A'. Wed May 02 15:25:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp827ala' Tue Jun 30 18:28:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp827Ala' O15164:p.Cys840Trp C840W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C W This feature has been corrected as a result of our quality control procedures. The original label was 'C840W'. Wed May 02 16:11:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys840trp' Tue Jun 30 16:45:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys840Trp' O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F979A/N980A'. Wed May 02 20:29:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe979ala,asn980ala' Tue Jun 30 18:53:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe979Ala;Asn980Ala]' ecolx Escherichia coli direct interaction direct interaction h4(127)-trim24-1 2 17 unspecified role unspecified role bait bait K5ac K5ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain K8ac K8ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain K12ac K12ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain K16ac K16ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain K20ac K20ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 16 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F979A/N980A'. Wed May 02 16:00:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe979ala,asn980ala' Tue Jun 30 19:07:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe979Ala;Asn980Ala]' PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(133)-trim24-7 8 20 unspecified role unspecified role unspecified role unspecified role Region 23-31 Region 23-31 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(133)-phf21a-1 3 19 unspecified role unspecified role prey prey PHD domain Region 488-535 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 20 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro direct interaction direct interaction h4(127)-trim24-2 4 17 unspecified role unspecified role unspecified role unspecified role Region 14-19 Region 14-19 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction trim24-trim24-1 11 16 unspecified role unspecified role unspecified role unspecified role Bromo domain Region 896-1016 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role PHD domain Region 824-886 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(133)-trim24-8 12 20 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 16 unspecified role unspecified role unspecified role unspecified role O15164:p.Cys840Trp C840W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C W This feature has been corrected as a result of our quality control procedures. The original label was 'C840W'. Wed May 02 19:12:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys840trp' Tue Jun 30 17:16:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys840Trp' O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F979A/N980A'. Wed May 02 18:41:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe979ala,asn980ala' Tue Jun 30 18:52:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe979Ala;Asn980Ala]' PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction