DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221353X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221354X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221358X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro decarboxxylation assay decarboxylation assay decarboxxylation assay predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221359X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221357X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221356X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221351X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221355X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot El Bakkouri M., Gutsche I., Kanjee U., Zhao B., Yu M., Goret G., Schoehn G., Burmeister WP., Houry WA. DIP original experiment: DIP-221352X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity. ldci_ecoli Inducible lysine decarboxylase cadA b4131 ldcI JW4092 protein protein ecoli Escherichia coli (strain K12) MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLKLIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISFFEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQKSPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTNGTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIPQSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWVPYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAFQKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK DIP protein P0A9H3 original sequence version: 58 This sequence has been withdrawn from Uniprot. B9776622A0AE0190 rava_ecoli ATPase RavA yieN Regulatory ATPase variant A ravA b3746 JW3725 protein protein ecoli Escherichia coli (strain K12) MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC DIP protein P31473 original sequence version: 98 DIP protein P31473 original sequence version: null 3D2EDCAA17240BE9 cada-4 2 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rava-1 7 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction cada-rava-1 4 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role region Δ336-433 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ336-433'. ecolx Escherichia coli direct interaction direct interaction cada-rava-2 5 10 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey LARA domain Region 329-440 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region Δ336-433 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ336-433'. ecolx Escherichia coli direct interaction direct interaction rava-cada-5 8 10 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association cada-rava-3 9 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association cada-rava-4 1 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rava-rava-4 3 11 unspecified role unspecified role prey prey LARA domain Region 329-440 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction Deletion of region 336-433 decreased the interaction rate/strength. rava-2 6 11 unspecified role unspecified role unspecified role unspecified role Region 1-335/434-498 Region 1-335/434-498 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction