DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structure (London, England : 1993) 2010 imex curation Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218306X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structure (London, England : 1993) 2010 imex curation Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218307X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structure (London, England : 1993) 2010 imex curation Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pennell S., Westcott S., Ortiz-Lombardía M., Patel D., Li J., Nott TJ., Mohammed D., Buxton RS., Yaffe MB., Verma C., Smerdon SJ. DIP original experiment: DIP-218308X Structure (London, England : 1993) 2010 IMEx Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. pthr Optimal peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DTAPTEKIAYKK fhaa_myctu FHA domain-containing protein FhaA fhaA TB39.8 Rv0020c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MGSQKRLVQRVERKLEQTVGDAFARIFGGSIVPQEVEALLRREAADGIQSLQGNRLLAPNEYIITLGVHDFEKLGADPELKSTGFARDLADYIQEQGWQTYGDVVVRFEQSSNLHTGQFRARGTVNPDVETHPPVIDCARPQSNHAFGAEPGVAPMSDNSSYRGGQGQGRPDEYYDDRYARPQEDPRGGPDPQGGSDPRGGYPPETGGYPPQPGYPRPRHPDQGDYPEQIGYPDQGGYPEQRGYPEQRGYPDQRGYQDQGRGYPDQGQGGYPPPYEQRPPVSPGPAAGYGAPGYDQGYRQSGGYGPSPGGGQPGYGGYGEYGRGPARHEEGSYVPSGPPGPPEQRPAYPDQGGYDQGYQQGATTYGRQDYGGGADYTRYTESPRVPGYAPQGGGYAEPAGRDYDYGQSGAPDYGQPAPGGYSGYGQGGYGSAGTSVTLQLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLADLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEIIVRMH 2642F9CF23FE7906 fha-pthr-1 1 4 unspecified role unspecified role unspecified role unspecified role pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain pthr:p.Thr5Ser T5S/Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T S Wed May 02 16:30:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr5ser' Tue Jun 30 18:00:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr5Ser' Region 1-8 Region 1-8 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pthr:p.Thr5Tyr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T Y Wed May 02 15:26:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr5tyr' Tue Jun 30 17:26:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr5Tyr' in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction fha-pthr-2 3 4 unspecified role unspecified role unspecified role unspecified role pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain Region 1-8 Region 1-8 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P71590:p.Ser473Ala S473A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S473A'. Wed May 02 16:10:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser473ala' Tue Jun 30 16:09:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser473Ala' P71590:p.Asn495Ala N495A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N495A'. Wed May 02 18:21:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn495ala' Tue Jun 30 19:11:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn495Ala' P71590:p.Arg459Ala R459A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R459A'. Wed May 02 15:33:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg459ala' Tue Jun 30 19:19:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg459Ala' ecolx Escherichia coli direct interaction direct interaction fha-pthr-3 2 4 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role FHA domain Region 430-527 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction