DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219070X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219067X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219068X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219065X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219066X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219069X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219072X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 2011 imex curation Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L., Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Cissé M., Halabisky B., Harris J., Devidze N., Dubal DB., Sun B., Orr A., Lotz G., Kim DH., Hamto P., Ho K., Yu GQ., Mucke L. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-219071X Nature 2011 IMEx Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. ubiq_rat Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb protein protein rat Rattus norvegicus (Rat) Rat MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair mint C42A35397FFD9B52 b2b9b0_rat Receptor protein-tyrosine kinase Ephb2 protein protein rat Rattus norvegicus (Rat) Rat MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRVIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLVLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGTIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGVVFVIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFTNAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEV DIP protein B2B9B0 original sequence version: 55 DIP protein B2B9B0 original sequence version: 31 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 p08592-pro_0000000163 Amyloid-beta protein 42 App ABPP Alzheimer disease amyloid A4 protein homolog Amyloid precursor protein Amyloidogenic glycoprotein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein rat Rattus norvegicus (Rat) Rat DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 68706EF3D7839B56 p12023-pro_0000000118 Amyloid-beta protein 42 App ABPP Alzheimer disease amyloid A4 protein homolog Amyloid precursor protein Amyloidogenic glycoprotein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 68706EF3D7839B56 ephb2_mouse Ephrin type-B receptor 2 Tyrosine-protein kinase receptor EPH-3 Neural kinase Nuk receptor tyrosine kinase Tyrosine-protein kinase receptor SEK-3 Ephb2 Epth3 Nuk Sek3 protein protein mouse Mus musculus Mouse MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEV DIP protein P54763 original sequence version: 151 DIP protein P54763 original sequence version: 119 DIP protein P54763 original sequence version: 123 92DB5234F18758A7 app-6 4 11 unspecified role unspecified role unspecified role unspecified role crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 11 unspecified role unspecified role unspecified role unspecified role crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster physical association physical association abeta142-ephb2-1 3 13 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-15894794]N-terminal positions must always be 1. Actual positions : 0-0 [EBI-15894794]0-0 in vitro In vitro 14 unspecified role unspecified role prey prey Fc-tag Fc-tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 27-548 Region 27-548 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain mouse Mus musculus Mouse direct interaction direct interaction app-7 5 11 unspecified role unspecified role unspecified role unspecified role crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 11 unspecified role unspecified role unspecified role unspecified role crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster physical association physical association ephb2-abeta142-1 1 10 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-15894828]N-terminal positions must always be 1. Actual positions : 0-0 [EBI-15894828]0-0 in vitro In vitro physical association physical association ub-ephb2-1 7 9 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat 10 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat physical association physical association ephb2-abeta142-2 8 13 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-15894874]N-terminal positions must always be 1. Actual positions : 0-0 [EBI-15894874]0-0 in vitro In vitro 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 192-994 Region 192-994 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association ephb2-abeta142-3 2 13 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-15894897]N-terminal positions must always be 1. Actual positions : 0-0 [EBI-15894897]0-0 in vitro In vitro 14 unspecified role unspecified role bait bait Fc-tag Fc-tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 27-548 Region 27-548 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain mouse Mus musculus Mouse direct interaction direct interaction abeta142-ephb2-2 6 13 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-15894920]N-terminal positions must always be 1. Actual positions : 0-0 [EBI-15894920]0-0 in vitro In vitro 14 unspecified role unspecified role prey prey Fc-tag Fc-tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 27-548 Region 27-548 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain mouse Mus musculus Mouse direct interaction direct interaction