DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220631X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions in vitro In vitro protein phosphatase assay protein phosphatase assay predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220632X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Huynh TN., Noriega CE., Stewart V. Only protein-protein interactions in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase predetermined predetermined participant predetermined Huynh TN., Noriega CE., Stewart V. DIP original experiment: DIP-220633X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX. narl_ecoli Nitrate/nitrite response regulator protein NarL narL frdR b1221 JW1212 protein protein ecoli Escherichia coli (strain K12) MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF DIP protein P0AF28 original sequence version: 83 DIP protein P0AF28 original sequence version: 60 7DED78E4EF7EC57D narx_ecoli Nitrate/nitrite sensor protein NarX narX narR b1222 JW1213 protein protein ecoli Escherichia coli (strain K12) MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE DIP protein P0AFA2 original sequence version: 44 DIP protein P0AFA2 original sequence version: 58 886BA0FC2C8F3C3E narx-2 1 5 putative self putative self putative self putative self P0AFA2:p.Gln404Ala Q404A/N/E mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 14:47:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404ala' Tue Jun 30 16:00:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Ala' P0AFA2:p.Asp400Glu D400E/N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D E Wed May 02 20:03:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp400glu' Tue Jun 30 17:39:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp400Glu' P0AFA2:p.Gln404Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Q N Wed May 02 15:10:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404asn' Tue Jun 30 18:54:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Asn' P0AFA2:p.Asp400Asn mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D N Wed May 02 14:50:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp400asn' Tue Jun 30 17:11:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp400Asn' P0AFA2:p.Gln404Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 19:39:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404glu' Tue Jun 30 20:06:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Glu' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true narl-narx-1 3 4 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli 5 phosphate donor phosphate donor unspecified role unspecified role ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer narl-narx-2 2 4 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role P0AFA2:p.Gln404Ala Q404A/E mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:54:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404ala' Tue Jun 30 15:36:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Ala' P0AFA2:p.Gln404His D404H/N mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q H Wed May 02 15:53:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404his' Tue Jun 30 17:26:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404His' P0AFA2:p.Asp400Ala D400A/S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:16:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp400ala' Tue Jun 30 17:40:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp400Ala' P0AFA2:p.[His399Gln;Gln404His] Q404H/H399Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q H certain certain sequence position certain certain certain sequence position certain H Q This feature has been corrected as a result of our quality control procedures. The original label was 'Q404H/H399Q'. Wed May 02 18:03:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his399gln,gln404his' Tue Jun 30 20:11:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His399Gln;Gln404His]' P0AFA2:p.His399Gln H399Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H Q This feature has been corrected as a result of our quality control procedures. The original label was 'H399Q'. Wed May 02 15:33:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his399gln' Tue Jun 30 18:32:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His399Gln' P0AFA2:p.[His399Gln;Asp400Ala] D400A/H399Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain H Q This feature has been corrected as a result of our quality control procedures. The original label was 'D400A/H399Q'. Wed May 02 18:22:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his399gln,asp400ala' Tue Jun 30 19:26:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His399Gln;Asp400Ala]' P0AFA2:p.[His399Gln;Gln404Ala] Q404A/H399Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain H Q This feature has been corrected as a result of our quality control procedures. The original label was 'Q404A/H399Q'. Wed May 02 16:44:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his399gln,gln404ala' Tue Jun 30 19:33:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His399Gln;Gln404Ala]' P0AFA2:p.Gln404Asn D404H/N mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q N Wed May 02 18:10:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404asn' Tue Jun 30 17:50:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Asn' P0AFA2:p.Asp400Ser mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D S Wed May 02 19:50:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp400ser' Tue Jun 30 18:19:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp400Ser' P0AFA2:p.Gln404Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 18:09:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln404glu' Tue Jun 30 16:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln404Glu' ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation