HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization. EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization. Infection and immunity 2010 imex curation Xie HX., Yu HB., Zheng J., Nie P., Foster LJ., Mok YK., Finlay BB., Leung KY. I selected human TUBA4A since the cell used in IP is human hek293a and searching sigma for mouse monoclonal anti-alpha-tubulin antibody showed its TUBA4A KaYin.Leung@twu.ca 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293a Homo sapiens hek293a hek293a anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xie HX., Yu HB., Zheng J., Nie P., Foster LJ., Mok YK., Finlay BB., Leung KY. I selected human TUBA4A since the cell used in IP is human hek293a and searching sigma for mouse monoclonal anti-alpha-tubulin antibody showed its TUBA4A KaYin.Leung@twu.ca KaYin .Leung@twu.ca Accepted 2017-AUG-24 AT 12:29 BST AT 12:29 BST by ORCHARD Infection and immunity 2010 imex curation EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization. EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization. Infection and immunity 2010 imex curation Xie HX., Yu HB., Zheng J., Nie P., Foster LJ., Mok YK., Finlay BB., Leung KY. I selected human TUBA4A since the cell used in IP is human hek293a and searching sigma for mouse monoclonal anti-alpha-tubulin antibody showed its TUBA4A KaYin.Leung@twu.ca 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Xie HX., Yu HB., Zheng J., Nie P., Foster LJ., Mok YK., Finlay BB., Leung KY. I selected human TUBA4A since the cell used in IP is human hek293a and searching sigma for mouse monoclonal anti-alpha-tubulin antibody showed its TUBA4A KaYin.Leung@twu.ca KaYin .Leung@twu.ca Accepted 2017-AUG-24 AT 12:29 BST AT 12:29 BST by ORCHARD Infection and immunity 2010 imex curation tba4a_human Tubulin alpha-4A chain TUBA4A Tubulin alpha-1 chain Alpha-tubulin 1 Testis-specific alpha-tubulin Tubulin H2-alpha TUBA1 protein protein human Homo sapiens Human MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE C00ED90A183FE8F2 q4g4d4_edwta eseG protein protein edwta Edwardsiella tarda MPWMPRLRCAMARPTATQAIHCCASGHRICWIRLINPKEPMMINGVFTDAPLPPTAAPGPCPAPSRREIGTQCDLLGIEAPRSRAPGASAPLDQLRAQAQEAEQKAKACGVDMAKRSFVAKVLTLVAASAVLITAALITGLTAGAGVPLLALASIGFTIAVGDVACAAYDWNHKKNGGEGLAMGSDAIGNAIHWLGKVSGASDERARQVAAYGSLLARSAITVSTVAVSGFLPVAAPLALQTTIPALTLGRTMIEISASAIKTNETHHGIVQRGHEINAGRAWGRVEIAQSEPTVDRDADERRIAAQLATLEKTRAFLIQTLRQNGVESAPPARRYSFA 2AFB16AEDE2E94CE q4g4d3_edwta escB protein protein edwta Edwardsiella tarda MTSAPLSTQAENVARRCLALLEGRETQRPLSGASQDEMEAGYARGCQALNDGDLREATATFAFLALQQPLERRFTFAFACALQQQQEYRQALTLFSYALALRANDPFTPFHMAECLFALREPDAAMDALDATIALCYGQADGNPGYTLLRQRAQDLLDTLNQP 9E2ECC0ADC978C85 escb-eseg-1 2 4 unspecified role unspecified role prey prey tem tag tem tag TEM1 β-lactamase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 4B tuba4a-eseg-1 1 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait physical association physical association 5B, 6