DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219960X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219962X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219963X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219961X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219959X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Takeshita D., Tomita K. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Takeshita D., Tomita K. DIP original experiment: DIP-219964X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Assembly of Q{beta} viral RNA polymerase with host translational elongation factors EF-Tu and -Ts. efts_ecoli Elongation factor Ts tsf Bacteriophage Q beta RNA-directed RNA polymerase subunit IV JW0165 b0170 protein protein ecoli Escherichia coli (strain K12) MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKSGAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVAGKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKGADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEVAAMSKQS DIP protein P0A6P1 original sequence version: 111 DIP protein P0A6P1 original sequence version: 72 0B9D21E928A5051C eftu1_ecoli Elongation factor Tu 1 tufA b3339 P-43 JW3301 Bacteriophage Q beta RNA-directed RNA polymerase subunit III protein protein ecoli Escherichia coli (strain K12) MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG DIP protein P0CE47 original sequence version: 51 DIP protein P0CE47 original sequence version: null 731A60255F43358F rdrp_bpqbe RNA-directed RNA polymerase subunit beta RNA replicase beta chain RNA-directed RNA polymerase subunit II protein protein bacteriophage q-beta Enterobacteria phage Qbeta MSKTASSRNSLSAQLRRAANTRIEVEGNLALSIANDLLLAYGQSPFNSEAECISFSPRFDGTPDDFRINYLKAEIMSKYDDFSLGIDTEAVAWEKFLAAEAECALTNARLYRPDYSEDFNFSLGESCIHMARRKIAKLIGDVPSVEGMLRHCRFSGGATTTNNRSYGHPSFKFALPQACTPRALKYVLALRASTHFDIRISDISPFNKAVTVPKNSKTDRCIAIEPGWNMFFQLGIGGILRDRLRCWGIDLNDQTINQRRAHEGSVTNNLATVDLSAASDSISLALCELLLPPGWFEVLMDLRSPKGRLPDGSVVTYEKISSMGNGYTFELESLIFASLARSVCEILDLDSSEVTVYGDDIILPSCAVPALREVFKYVGFTTNTKKTFSEGPFRESCGKHYYSGVDVTPFYIRHRIVSPADLILVLNNLYRWATIDGVWDPRAHSVYLKYRKLLPKQLQRNTIPDGYGDGALVGSVLINPFAKNRGWIRYVPVITDHTRDRERAELGSYLYDLFSRCLSESNDGLPLRGPSGCDSADLFAIDQLICRSNPTKISRSTGKFDIQYIACSSRVLAPYGVFQGTKVASLHEA This sequence has been withdrawn from Uniprot. 8C02DB50F7202DEF 8C02DB50F7202DEF eftu-qbeta-1 5 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli association association tsf-eftu-1 3 8 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction eftu-qbeta-2 4 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0CE47:p.Phe262Ala F262A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F262A'. Wed May 02 16:47:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe262ala' Tue Jun 30 17:06:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe262Ala' P0CE47:p.Phe262_Arg263del Δ262-263 mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain FR .. Wed May 02 17:46:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe_arg262-263del_del' Tue Jun 30 18:51:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe262_Arg263del' ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association tsf-qbeta-1 6 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region Δ187-226 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ187-226'. ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association tsf-eftu-2 1 8 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli association association eftu-tsf-1 2 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction