DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature 2010 imex curation Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219405X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature 2010 imex curation Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219404X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature 2010 imex curation Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219402X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature 2010 imex curation Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Elden AC., Kim HJ., Hart MP., Chen-Plotkin AS., Johnson BS., Fang X., Armakola M., Geser F., Greene R., Lu MM., Padmanabhan A., Clay-Falcone D., McCluskey L., Elman L., Juhr D., Gruber PJ., Rüb U., Auburger G., Trojanowski JQ., Lee VM., Van Deerlin VM., Bonini NM., Gitler AD. DIP original experiment: DIP-219403X Nature 2010 IMEx Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. tadbp_human TAR DNA-binding protein 43 TARDBP TDP43 protein protein human Homo sapiens Human MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM DIP protein Q13148 original sequence version: 147 DIP protein Q13148 original sequence version: 114 DIP protein Q13148-1 original sequence version: 169 DIP protein Q13148-1 original sequence version: 124 8E09A1206FB4EF4A atx2_human Ataxin-2 Spinocerebellar ataxia type 2 protein Trinucleotide repeat-containing gene 13 protein SCA2 TNRC13 ATXN2 ATX2 protein protein human Homo sapiens Human MRSAAAAPRSPAVATESRRFAAARWPGWRSLQRPARRSGRGGGGAAPGPYPSAAPPPPGPGPPPSRQSSPPSASDCFGSNGNGGGAFRPGSRRLLGLGGPPRPFVVLLLPLASPGAPPAAPTRASPLGARASPPRSGVSLARPAPGCPRPACEPVYGPLTMSLKPQQQQQQQQQQQQQQQQQQQQQQQPPPAAANVRKPGGSGLLASPAAAPSPSSSSVSSSSATAPSSVVAATSGGGRPGLGRGRNSNKGLPQSTISFDGIYANMRMVHILTSVVGSKCEVQVKNGGIYEGVFKTYSPKCDLVLDAAHEKSTESSSGPKREEIMESILFKCSDFVVVQFKDMDSSYAKRDAFTDSAISAKVNGEHKEKDLEPWDAGELTANEELEALENDVSNGWDPNDMFRYNEENYGVVSTYDSSLSSYTVPLERDNSEEFLKREARANQLAEEIESSAQYKARVALENDDRSEEEKYTAVQRNSSEREGHSINTRENKYIPPGQRNREVISWGSGRQNSPRMGQPGSGSMPSRSTSHTSDFNPNSGSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSSPACKQEKDDKEEKKDAAEQVRKSTLNPNAKEFNPRSFSQPKPSTTPTSPRPQAQPSPSMVGHQQPTPVYTQPVCFAPNMMYPVPVSPGVQPLYPIPMTPMPVNQAKTYRAVPNMPQQRQDQHHQSAMMHPASAAGPPIAATPPAYSTQYVAYSPQQFPNQPLVQHVPHYQSQHPHVYSPVIQGNARMMAPPTHAQPGLVSSSATQYGAHEQTHAMYACPKLPYNKETSPSFYFAISTGSLAQQYAHPNATLHPHTPHPQPSATPTGQQQSQHGGSHPAPSPVQHHQHQAAQALHLASPQQQSAIYHAGLAPTPPSMTPASNTQSPQNSFPAAQQTVFTIHPSHVQPAYTNPPHMAHVPQAHVQSGMVPSHPTAHAPMMLMTTQPPGGPQAALAQSALQPIPVSTTAHFPYMTHPSVQAHHQQQL DIP protein Q99700 original sequence version: 90 40A2883FF9D5D118 pbp1_yeast PAB1-binding protein 1 PBP1 MRS16 Poly(A)-binding protein-binding protein YGR178C protein protein yeast Saccharomyces cerevisiae Baker's yeast MKGNFRKRDSSTNSRKGGNSDSNYTNGGVPNQNNSSMFYENPEITRNFDDRQDYLLANSIGSDVTVTVTSGVKYTGLLVSCNLESTNGIDVVLRFPRVADSGVSDSVDDLAKTLGETLLIHGEDVAELELKNIDLSLDEKWENSKAQETTPARTNIEKERVNGESNEVTKFRTDVDISGSGREIKERKLEKWTPEEGAEHFDINKGKALEDDSASWDQFAVNEKKFGVKSTFDEHLYTTKINKDDPNYSKRLQEAERIAKEIESQGTSGNIHIAEDRGIIIDDSGLDEEDLYSGVDRRGDELLAALKSNSKPNSNKGNRYVPPTLRQQPHHMDPAIISSSNSNKNENAVSTDTSTPAAAGAPEGKPPQKTSKNKKSLSSKEAQIEELKKFSEKFKVPYDIPKDMLEVLKRSSSTLKSNSSLPPKPISKTPSAKTVSPTTQISAGKSESRRSGSNISQGQSSTGHTTRSSTSLRRRNHGSFFGAKNPHTNDAKRVLFGKSFNMFIKSKEAHDEKKKGDDASENMEPFFIEKPYFTAPTWLNTIEESYKTFFPDEDTAIQEAQTRFQQRQLNSMGNAVPGMNPAMGMNMGGMMGFPMGGPSASPNPMMNGFAAGSMGMYMPFQPQPMFYHPSMPQMMPVMGSNGAEEGGGNISPHVPAGFMAAGPGAPMGAFGYPGGIPFQGMMGSGPSGMPANGSAMHSHGHSRNYHQTSHHGHHNSSTSGHK 92005F3A2346193E 92005F3A2346193E pbp1-tardbp-1 3 7 unspecified role unspecified role bait bait CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association atxn2-tardbp-1 2 6 unspecified role unspecified role bait bait human Homo sapiens Human 5 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association tardbp-atxn2-1 4 6 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q13148:p.Phe229Leu F229L mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F L This feature has been corrected as a result of our quality control procedures. The original label was 'F229L'. Wed May 02 19:00:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe229leu' Tue Jun 30 16:28:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe229Leu' Q13148:p.[Phe147Leu;Phe149Leu] F147L/F149L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L This feature has been corrected as a result of our quality control procedures. The original label was 'F147L/F149L'. Wed May 02 20:12:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe147leu,phe149leu' Tue Jun 30 19:44:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe147Leu;Phe149Leu]' Q13148:p.[Phe194Leu;Phe229Leu;Phe231Leu] F194L/F229L/F231L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L This feature has been corrected as a result of our quality control procedures. The original label was 'F194L/F229L/F231L'. Wed May 02 15:36:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe194leu,phe229leu,phe231leu' Tue Jun 30 16:19:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe194Leu;Phe229Leu;Phe231Leu]' Q13148:p.[Phe147Leu;Phe149Leu;Phe194Leu;Phe229Leu;Phe231Leu] F147L/F149L/F194L/F229L/F231L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L certain certain sequence position certain certain certain sequence position certain F L This feature has been corrected as a result of our quality control procedures. The original label was 'F147L/F149L/F194L/F229L/F231L'. Wed May 02 14:44:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe147leu,phe149leu,phe194leu,phe229leu,phe231leu' Tue Jun 30 19:36:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe147Leu;Phe149Leu;Phe194Leu;Phe229Leu;Phe231Leu]' human Homo sapiens Human physical association physical association tardbp-atxn2-2 1 6 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association