DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Hayama R., Marians KJ. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Hayama R., Marians KJ. DIP original experiment: DIP-220504X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Hayama R., Marians KJ. Only protein-protein interactions ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hayama R., Marians KJ. DIP original experiment: DIP-220502X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Hayama R., Marians KJ. Only protein-protein interactions ecolx Escherichia coli anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hayama R., Marians KJ. DIP original experiment: DIP-220503X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. mukb_ecoli Chromosome partition protein MukB mukB b0924 JW0907 Structural maintenance of chromosome-related protein protein protein ecoli Escherichia coli (strain K12) MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTMAAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVVGVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAMEGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLRDYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMRHANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAASDHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQQENEARAEAAELEVDELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAKELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQRHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARIASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTSSQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFGGVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGDPQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLSERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHENDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQFGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSYSDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDTKKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAEMDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDLRSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIRTDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGLQNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLNPQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSILVMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPENISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS DIP protein P22523 original sequence version: 108 DIP protein P22523 original sequence version: 122 DIP protein P22523 original sequence version: null 38C7874BEB78D6D6 parc_ecoli DNA topoisomerase 4 subunit A Topoisomerase IV subunit A parC b3019 JW2987 protein protein ecoli Escherichia coli (strain K12) MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRRIVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPARLPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTEAEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRNKKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGLDGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEIIRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGILASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMGWVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQGEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITLPENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARGEDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEIDSPRRASSGDSEE DIP protein P0AFI2 original sequence version: 41 DIP protein P0AFI2 original sequence version: 55 0D4907E96CEE7086 mukb-parc-2 2 5 unspecified role unspecified role prey prey ecolx Escherichia coli 4 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association parc-mukb-3 3 5 unspecified role unspecified role bait bait ecolx Escherichia coli 4 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association parc-mukb-4 1 5 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminal domain Region 497-752 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region Δ696-752 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain This feature has been corrected as a result of our quality control procedures. The original label was 'Δ696-752'. P0AFI2:p.[Arg705Glu;Arg729Ala] R705E/R729A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R705E/R729A'. Wed May 02 14:59:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg705glu,arg729ala' Tue Jun 30 17:44:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg705Glu;Arg729Ala]' P0AFI2:p.Arg705Glu R705E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R705E'. Wed May 02 19:05:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg705glu' Tue Jun 30 18:12:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg705Glu' P0AFI2:p.Arg729Ala R729A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R729A'. Wed May 02 19:13:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg729ala' Tue Jun 30 15:39:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg729Ala' ecolx Escherichia coli 4 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction