DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254154X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254151X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254148X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254150X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254152X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254155X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254153X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Moretto-Zita M., Jin H., Shen Z., Zhao T., Briggs SP., Xu Y. DIP original experiment: DIP-254149X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 pin1_mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 protein protein mouse Mus musculus Mouse MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGGSTVGGSSKNGQGEPAKVRCSHLLVKHSQSRRPSSWRQEKITRSKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGPFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q9QUR7 original sequence version: 81 188E95F009176B1F pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 nanog_mouse Homeobox protein NANOG Nanog Ecat4 Enk Homeobox transcription factor Nanog Early embryo specific expression NK-type homeobox protein ES cell-associated protein 4 protein protein mouse Mus musculus Mouse MSVGLPGPHSLPSSEEASNSGNASSMPAVFHPENYSCLQGSATEMLCTEAASPRPSSEDLPLQGSPDSSTSPKQKLSSPEADKGPEEEENKVLARKQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCKRWQKNQWLKTSNGLIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTAQSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI DIP protein Q80Z64 original sequence version: 43 DIP protein Q80Z64 original sequence version: 64 DIP protein Q80Z64 original sequence version: 77 DIP protein Q80Z64 original sequence version: 78 4EF96408B767C79F q80z64-1 Homeobox protein NANOG Nanog Ecat4 Enk ES cell-associated protein 4 Early embryo specific expression NK-type homeobox protein Homeobox transcription factor Nanog protein protein mouse Mus musculus Mouse MSVGLPGPHSLPSSEEASNSGNASSMPAVFHPENYSCLQGSATEMLCTEAASPRPSSEDLPLQGSPDSSTSPKQKLSSPEADKGPEEEENKVLARKQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCKRWQKNQWLKTSNGLIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTAQSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI DIP protein Q80Z64-1 original sequence version: 43 DIP protein Q80Z64-1 original sequence version: 77 nanog-pin1-1 3 10 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association pin1-nanog-1 5 13 unspecified role unspecified role prey prey Q80Z64-1:p.[Ser52Glu;Ser65Glu;Ser71Glu] S52E/S65E/S71E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E This feature has been corrected as a result of our quality control procedures. The original label was 'S52E/S65E/S71E'. Wed May 02 16:08:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser52glu,ser65glu,ser71glu' Tue Jun 30 18:56:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser52Glu;Ser65Glu;Ser71Glu]' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association mub-nanog-1 1 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 9 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal human Homo sapiens Human physical association physical association pin1-nanog-2 8 10 unspecified role unspecified role bait bait mouse Mus musculus Mouse 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association nanog-pin1-2 2 10 unspecified role unspecified role prey prey mouse Mus musculus Mouse 13 unspecified role unspecified role bait bait Q80Z64-1:p.[Ser52Ala;Ser65Ala;Ser71Ala;Thr287Ala] S52A/S65A/S71A/T287A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'S52A/S65A/S71A/T287A'. Wed May 02 18:16:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser52ala,ser65ala,ser71ala,thr287ala' Tue Jun 30 15:34:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser52Ala;Ser65Ala;Ser71Ala;Thr287Ala]' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse Mus musculus Mouse physical association physical association nanog-mub-1 7 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 13 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse Mus musculus Mouse physical association physical association mub-nanog-2 6 13 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 9 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal human Homo sapiens Human physical association physical association pin1-nanog-3 4 13 unspecified role unspecified role prey prey Q80Z64-1:p.Ser71Ala S71A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S71A'. Wed May 02 16:30:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser71ala' Tue Jun 30 17:13:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser71Ala' Q80Z64-1:p.[Ser52Ala;Ser65Ala] S52A/S65A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S52A/S65A'. Wed May 02 16:03:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser52ala,ser65ala' Tue Jun 30 18:25:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser52Ala;Ser65Ala]' Q80Z64-1:p.[Ser52Ala;Ser65Ala;Ser71Ala] S52A/S65A/S71A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S52A/S65A/S71A'. Wed May 02 17:04:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser52ala,ser65ala,ser71ala' Tue Jun 30 16:33:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser52Ala;Ser65Ala;Ser71Ala]' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association