DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Nature structural & molecular biology 2010 imex curation Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. DIP original experiment: DIP-217568X Nature structural & molecular biology 2010 IMEx Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Nature structural & molecular biology 2010 imex curation Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. Only protein-protein interactions human Homo sapiens Human 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. DIP original experiment: DIP-217569X Nature structural & molecular biology 2010 IMEx Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. Nature structural & molecular biology 2010 imex curation Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Phelan CA., Gampe RT., Lambert MH., Parks DJ., Montana V., Bynum J., Broderick TM., Hu X., Williams SP., Nolte RT., Lazar MA. DIP original experiment: DIP-217570X Nature structural & molecular biology 2010 IMEx Structure of Rev-erbalpha bound to N-CoR reveals a unique mechanism of nuclear receptor-co-repressor interaction. nr1d1_human Nuclear receptor subfamily 1 group D member 1 NR1D1 EAR1 HREV THRAL Rev-erbA-alpha V-erbA-related protein 1 protein protein human Homo sapiens Human MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ DIP protein P20393 original sequence version: 124 DIP protein P20393 original sequence version: 162 DIP protein P20393 original sequence version: 132 67C71758E166508A ncor1_human Nuclear receptor corepressor 1 NCOR1 KIAA1047 protein protein human Homo sapiens Human MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKPVSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKTNQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTREYYEKQFPEIRKQREQQERFQRVGQRGAGLSATIARSEHEISEIIDGLSEQENNEKQMRQLSVIPPMMFDAEQRRVKFINMNGLMEDPMKVYKDRQFMNVWTDHEKEIFKDKFIQHPKNFGLIASYLERKSVPDCVLYYYLTKKNENYKALVRRNYGKRRGRNQQIARPSQEEKVEEKEEDKAEKTEKKEEEKKDEEEKDEKEDSKENTKEKDKIDGTAEETEEREQATPRGRKTANSQGRRKGRITRSMTNEAAAASAAAAAATEEPPPPLPPPPEPISTEPVETSRWTEEEMEVAKKGLVEHGRNWAAIAKMVGTKSEAQCKNFYFNYKRRHNLDNLLQQHKQKTSRKPREERDVSQCESVASTVSAQEDEDIEASNEEENPEDSEVEAVKPSEDSPENATSRGNTEPAVELEPTTETAPSTSPSLAVPSTKPAEDESVETQVNDSISAETAEQMDVDQQEHSAEEGSVCDPPPATKADSVDVEVRVPENHASKVEGDNTKERDLDRASEKVEPRDEDLVVAQQINAQRPEPQSDNDSSATCSADEDVDGEPERQRMFPMDSKPSLLNPTGSILVSSPLKPNPLDLPQLQHRAAVIPPMVSCTPCNIPIGTPVSGYALYQRHIKAMHESALLEEQRQRQEQIDLECRSSTSPCGTSKSPNREWEVLQPAPHQVITNLPEGVRLPTTRPTRPPPPLIPSSKTTVASEKPSFIMGGSISQGTPGTYLTSHNQASYTQETPKPSVGSISLGLPRQQESAKSATLPYIKQEEFSPRSQNSQPEGLLVRAQHEGVVRGTAGAIQEGSITRGTPTSKISVESIPSLRGSITQGTPALPQTGIPTEALVKGSISRMPIEDSSPEKGREEAASKGHVIYEGKSGHILSYDNIKNAREGTRSPRTAHEISLKRSYESVEGNIKQGMSMRESPVSAPLEGLICRALPRGSPHSDLKERTVLSGSIMQGTPRATTESFEDGLKYPKQIKRESPPIRAFEGAITKGKPYDGITTIKEMGRSIHEIPRQDILTQESRKTPEVVQSTRPIIEGSISQGTPIKFDNNSGQSAIKHNVKSLITGPSKLSRGMPPLEIVPENIKVVERGKYEDVKAGETVRSRHTSVVSSGPSVLRSTLHEAPKAQLSPGIYDDTSARRTPVSYQNTMSRGSPMMNRTSDVTISSNKSTNHERKSTLTPTQRESIPAKSPVPGVDPVVSHSPFDPHHRGSTAGEVYRSHLPTHLDPAMPFHRALDPAAAAYLFQRQLSPTPGYPSQYQLYAMENTRQTILNDYITSQQMQVNLRPDVARGLSPREQPLGLPYPATRGIIDLTNMPPTILVPHPGGTSTPPMDRITYIPGTQITFPPRPYNSASMSPGHPTHLAAAASAEREREREREKERERERIAAASSDLYLRPGSEQPGRPGSHGYVRSPSPSVRTQETMLQQRPSVFQGTNGTSVITPLDPTAQLRIMPLPAGGPSISQGLPASRYNTAADALAALVDAAASAPQMDVSKTKESKHEAARLEENLRSRSAAVSEQQQLEQKTLEVEKRSVQCLYTSSAFPSGKPQPHSSVVYSEAGKDKGPPPKSRYEEELRTRGKTTITAANFIDVIITRQIASDKDARERGSQSSDSSSSLSSHRYETPSDAIEVISPASSPAPPQEKLQTYQPEVVKANQAENDPTRQYEGPLHHYRPQQESPSPQQQLPPSSQAEGMGQVPRTHRLITLADHICQIITQDFARNQVSSQTPQQPPTSTFQNSPSALVSTPVRTKTSNRYSPESQAQSVHHQRPGSRVSPENLVDKSRGSRPGKSPERSHVSSEPYEPISPPQVPVVHEKQDSLLLLSQRGAEPAEQRNDARSPGSISYLPSFFTKLENTSPMVKSKKQEIFRKLNSSGGGDSDMAAAQPGTEIFNLPAVTTSGSVSSRGHSFADPASNLGLEDIIRKALMGSFDDKVEDHGVVMSQPMGVVPGTANTSVVTSGETRREEGDPSPHSGGVCKPKLISKSNSRKSKSPIPGQGYLGTERPSSVSSVHSEGDYHRQTPGWAWEDRPSSTGSTQFPYNPLTMRMLSSTPPTPIACAPSAVNQAAPHQQNRIWEREPAPLLSAQYETLSDSDD DIP protein O75376 original sequence version: 186 DIP protein O75376 original sequence version: 75 DIP protein O75376 original sequence version: 153 DIP protein O75376 original sequence version: 147 DIP protein O75376 original sequence version: 112 DIP protein O75376-1 original sequence version: 111 DIP protein O75376 original sequence version: 146 1647FE060373A125 ncor1-nr1d1-1 1 5 unspecified role unspecified role unspecified role unspecified role Interaction domain 1 Region 2045-2065 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role unspecified role unspecified role Ligand-binding domain Region 281-323/423-614 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ncor1-nr1d1-2 3 5 unspecified role unspecified role fluorescence donor fluorescence donor Interaction domain 1 Region 2045-2065 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O75376:p.His2054Ala H2054A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H2054A'. Wed May 02 15:04:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his2054ala' Tue Jun 30 17:25:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His2054Ala' O75376:p.His2046Ala H2046A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H2046A'. Wed May 02 19:06:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his2046ala' Tue Jun 30 19:33:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His2046Ala' O75376:p.Leu2048Ala L2048A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L2048A'. Wed May 02 15:24:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu2048ala' Tue Jun 30 18:03:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu2048Ala' O75376:p.Leu2051Ala L2051A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L2051A'. Wed May 02 19:42:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu2051ala' Tue Jun 30 19:21:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu2051Ala' Region 2124-2149 Region 2124-2149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Allophycocyanin-tag Allophycocyanin-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Ligand-binding domain Region 281-614 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Europium-chelate-tag Europium-chelate-tag fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction ncor1-nr1d1-3 2 5 unspecified role unspecified role bait bait Interaction domain 1 Region 2045-2065 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O75376:p.Ile2055Ala I2055A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I2055A'. Wed May 02 17:52:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile2055ala' Tue Jun 30 18:11:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile2055Ala' O75376:p.Ile2058Ala I2058A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I2058A'. Wed May 02 19:02:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile2058ala' Tue Jun 30 20:10:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile2058Ala' O75376:p.Ile2059Ala I2059A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I2059A'. Wed May 02 17:48:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile2059ala' Tue Jun 30 16:20:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile2059Ala' O75376:p.[Ile2058Ala;Ile2059Ala] I2058A/I2059A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I2058A/I2059A'. Wed May 02 16:01:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile2058ala,ile2059ala' Tue Jun 30 17:16:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile2058Ala;Ile2059Ala]' human Homo sapiens Human 4 unspecified role unspecified role prey prey Ligand-binding domain Region 281-614 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association