DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature 2010 imex curation Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. DIP original experiment: DIP-220746X Nature 2010 IMEx Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature 2010 imex curation Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. DIP original experiment: DIP-220749X Nature 2010 IMEx Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature 2010 imex curation Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. DIP original experiment: DIP-220747X Nature 2010 IMEx Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature 2010 imex curation Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Alvarez SE., Harikumar KB., Hait NC., Allegood J., Strub GM., Kim EY., Maceyka M., Jiang H., Luo C., Kordula T., Milstien S., Spiegel S. DIP original experiment: DIP-220748X Nature 2010 IMEx Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL DIP protein Q12933 original sequence version: 131 DIP protein Q12933 original sequence version: 129 DIP protein Q12933 original sequence version: 167 DIP protein Q12933 original sequence version: 193 DIP protein Q12933-1 original sequence version: 154 DIP protein Q12933 original sequence version: 130 DIP protein Q12933 original sequence version: 151 C508BE185B783B20 ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN DIP protein Q13546 original sequence version: 158 DIP protein Q13546-1 original sequence version: 140 DIP protein Q13546 original sequence version: 146 DIP protein Q13546-1 original sequence version: 146 DIP protein Q13546 original sequence version: 134 DIP protein Q13546 original sequence version: 109 976E2428D525A9B2 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ripk1-ub-1 1 7 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association traf2-ub-1 2 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction ripk1-ub-2 3 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ripk1-ub-3 4 7 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction