DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Missra A., Gilmour DS. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Missra A., Gilmour DS. DIP original experiment: DIP-221251X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Missra A., Gilmour DS. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Missra A., Gilmour DS. DIP original experiment: DIP-221252X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Missra A., Gilmour DS. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Missra A., Gilmour DS. DIP original experiment: DIP-221253X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. nelfb_drome Negative elongation factor B NELF-B CG32721 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MIMSTPAKNNNGTGLEDVNIPGQAYLREALTSCTDPLKAIESFQLENGVLLPSLRPMLPLLDLHGVRRLDFHTSLMEELRDKLIAHINEMGQKEPRERDKKLKELLVKSFPVVRVKSLRPVVMAILRNTQHIDDKYLKILVRDRELYADTDTEVKRQIWRDNQSLFGDEVSPLLSQYIREKEHILFDHTNLNNLFFHPTPKVRRQGEVVQKLANMIGTSVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMALHDLEVQEIISIDPCHKFTWCLDACIREKNVDIKRSRELQGFLDNIKRGQEQVLGDLSMTLCDPYAINFLATSAIKILHHLINNEGMPRDNQILILLLRMLALGLSAWVMIDSQDFKEPKLDCQVVTKFLPALMSLMVDDQCRSLHAKLPPDERESALTTIEHSGPAPDAVEAYIQESSVASILAMYYTLHTARLKDRVGVLRVLAILSACKDDRAYEDPFLHSLIALLIPMSEEFATEDFCTTLFDEFIFAGLTRENVTSRHMLKLLWYVHNKLPAGRLATLMKAMQPTTAHNEHIHKLYEILQERIGTGAAETPVIEAPPMEFDSPLKSVPTPGPHYNVQ DIP protein Q9Y113 original sequence version: 68 D5A87283888A97F4 nelfa_drome Negative elongation factor A Nelf-A CG5874 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MANVRDSDTSLWLHNKLGTSNDSWINGSICSQLNKEVLRNIKECFPDLQTQVKLKLLLSFLHIPRRLVEEWKAELEEVIEVAGLDSELWVSMLAETMKTFPATSSLNTEISDYEDTRPIFIDMVNDLRKLVTKHSDLGMLPLECQYLNKNALISVVGQQPAPVKHFTLKRKPKSAQLRTELLHKSADAQSSLKKASAPTIPLRSRGMPRKMTDTTPLKGIPSRMPTTGFRSATVPGNAAQRPNLSRTPAGRKDGGIKLIEFTEQPLGYAAAKKRKREQQLEEQQKKQEQKQQQAAAAAAAAATAAASNAGDSSPTDAATASGGETPVTPTSANNSFEIKMEPQQLNQSAGSLEEPQDDEMSGKANMECDIETPEYATATLDFAQATSTSVADGQPGKPAVETKLKTPRTPKSAAKLNNNNNNNNNNSFNHTPKRIKQEIEIKSEEIIVPASIKLEKIETSPSTQRVSIQQQPPSLVQRTPHLLIRSSPQKRQNNGATTSAGTTTTTVGNTTIKMEKLDIKPMIRATGVSPSTSAGTTTTLLTPQQLRQAANPLANLPNNISVKITSAKAKAAAAAAATSSGSQTQSQQPQTLQVQQAPQQSHPPLLINSSTPVILASSPSAQRAKALALPSSSTSATTTTTIPSQAIKTMPLSQLKTATNSGPVIISQTIIQPAKRAQQQAGTSSAAAASQQQQQQQSQQQLLHQQIQQQTQQQAQLPQQPQQQQTQYILATPQQQPQQIQQQQQQQQPILPTLTSFSHSRPMPQTTTLYQATTPSGSGQTPTKILLKTSGSSSVVMTPLRQAQPQQATAVVSSNPPPLVATSAAVVSPGQTTLNIQNVQLPNRPVTIQPASQAAQQQHMQAQLQQQQPHPQHTIVANTTATQQPKLSQVLMQPTAAVGTSGVSALNVSPTSGKNKTIILTQKGVILRNIGGDMYQQIPISNVGNMQGLGGTTLMTTTAGPPSLVKTTPSSGVQLQQQQSGKQILPTLIPTSSLGGQHVIVQQQQPTNVIGNSQQQTIIRPVMTNVGGGLTTLPQGLTLIQRPGQQPQLVQVQAAPGSTQRTIITQSNTAAAASQQQPRQQQQQILVQHKPAPTLQQRLVTSTTSGGQGQQGNPNAGLPRTVQVQVQAQQQQQPQQQQATQQQSQQAPQRRGLSLSNEHVHKAHEMFRKANRVSRPDKALILGFMAGLRENPRPNNENVLVIKLGETEEKVQQDNGHTALCLVESHIRLDYNTGEWKTFQNYRLQDQSAAS DIP protein Q86NP2 original sequence version: 54 6A959443D06622D4 nelfe_drome Negative elongation factor E Nelf-E anon-66Da CG5994 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTSFKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEPRRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFLL DIP protein P92204 original sequence version: 86 9F1F351727D53C47 nelfd_drome Negative elongation factor D TH1 NELF-D CG9984 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEVEYDDSGWQGRAKGQTNPEETLEDNPQKTIQECLEKFLTPDYIMEPGIFTQLKRYFQSGGSPEEVISMLSENYKAVAQMANLLAEWLILAGVKVTEVQAMVENHLKEMILKSFDPKKADTIFTEEGETPDWLTEMIDHYTWRSLIYRLAEEYPDCLMLNFTIKLISDAGFQSEITSISTAAQQIEVFSRVLKTSIVKFLNNPDDVHGAIQECARMVCHGQHTYVYSQVLIQVLSQEQKGGFNMKRLSQEIIKYALQNNQNVTPITMALNGSAVYPQACQALTSMLTRNTLNPADITVLFRNYSGSDPPPIDLIRNPQFLELLVDALFRSGVKINPEHKPKYMFLLAYASAVIDQPAKKRPMTERMLNKEELKSTIQAIEKAHTICNVDQGSTELIAELQTLYNCIKYPVVGVGVIRWIENVVMEPSYFKLSTDSCPTHLAVLDEVAAVHPTLQQQILFLLIRLFESKQDELEILVQLEMKKMILDRMVNLLTRGCVVPVLRYIKQCCAIEDTDISLIRYFVTEVLETITHPYSPEFVQLFLPMVENEEITGTMRGEGDNDPVSEFIVHCKAHYTTV DIP protein Q24134 original sequence version: 74 2949EE378AEC19BE spt5h_drome Transcription elongation factor SPT5 Spt5 CG7626 dSpt5 DRB sensitivity-inducing factor large subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSDSEVSNMSDSGSEDGSISNKSQRSARSKSRSRSRSGSRGSRSVSRSRSRSQSGHSRSGSESPQRRDNRGKSDESGEEEEEPPGEDIDSEEYDEEENDDHPRKKKKKERFGGFIIDEAEVDDEVDEDDEWEEGANEIGIVGNEIDELGPTARDIEIRRRGTNLWDTQKEDEIEEYLRKKYADESIAKRHFGDGGEEMSDEITQQTLLPGIKDPNLWMVKCRIGEEKATALLLMRKYLTYLNTDDPLQIKSIIAPEGVKGYIYLEAYKQTHVKTCIDNVGNLRMGKWKQEMVPIKEMTDVLKVVKEQVGLKVKQWVRLKRGLYKDDIAQVDYVDLAQNQVHLKLLPRIDYTRMRGALRTTATESDDSKRKKKRRPAAKPFDPEAVRAIGGEVHSDGDFLLFEGNRYSRKGFLYKNFTMSAILSDGVKPTLAELERFEESPEEVNLEIMGTVKDDPTMAHSFSMGDNVEVCVGDLENLQAKIVAIDGTMITVMPKHQDLKDPLIFKASELRKYFKTGDHARVLAGRYEGETGLIIRVEPTRVVLVSDLTNHELEVLPRDLQLCSDVATGVDCLGQFQWGDMVQLDSQNVGVIVRLERENFHVLGMNGKCIECKPTALHKRKENRHTVALDADQNQIRRRDVVKVMEGPHAGRSGEIKHLYRSLAFLHCRMYTENGGIFVCKTRHLQLAGGSKTTVSNAGIVGGLGFMSPRIQSPMHPSGGRGARGGARGGRGGFRVTRDREILGKTIKISGGPYKGAVGIVKDATESTARVELHTSCQTISVDRNHIAIVGVTGKEGSVSTYGRTPARTPGYGAQTPSYTAAGSKTPLVGSQTPNWDTDTRTPYGTMTPSHDGSMTPRHGAWDPTANTTPARNNDFDYSLEEPSPSPGYNPSTPGYQMTSQFAPQTPGTLYGSDRSYSPFNPSPSPAPSPYPVGYMNTPSPSTYSPNTPGGIPQSPYNPQTPGASLDSSMGDWCTTDIEVRIHTHDDTDLVGQTGIIRTVSNGVCSVFLRQEDRSVSIVSEHLAPVLPCNGDEFKIIYGDDRESVGRVLSKDGDVFVCRINEEIKLLPINFLCKMKSID DIP protein Q9V460 original sequence version: 85 0B917AEE0C0ADD13 spt4h_drome Transcription elongation factor SPT4 spt4 CG12372 dSpt4 DRB sensitivity-inducing factor small subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAFDAIPKDLRGLRACLVCSLVKSFDQFETDGCENCEEFLRMKNNKDNVYDHTSNNFDGIIALTTPTDSWVAKWQRLSRFTRGIYAISVSGTLPQSTLRDMKNRGIVYKSRDRSQR DIP protein Q9TVQ5 original sequence version: 70 A4EF4580DCAA8A57 th1-nelf_a-1 1 6 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 4 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly association association spt5-spt4-7 3 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association spt4-spt5-5 2 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association