DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nature structural & molecular biology 2010 imex curation Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap predetermined predetermined participant predetermined Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. DIP original experiment: DIP-217485X Nature structural & molecular biology 2010 IMEx A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nature structural & molecular biology 2010 imex curation Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. DIP original experiment: DIP-217483X Nature structural & molecular biology 2010 IMEx A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nature structural & molecular biology 2010 imex curation Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Aguilar PS., Fröhlich F., Rehman M., Shales M., Ulitsky I., Olivera-Couto A., Braberg H., Shamir R., Walter P., Mann M., Ejsing CS., Krogan NJ., Walther TC. DIP original experiment: DIP-217484X Nature structural & molecular biology 2010 IMEx A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. pkh1_yeast Serine/threonine-protein kinase PKH1 3-phosphoinositide-dependent protein kinase 1 PKH1 YDR490C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGNRSLTEADHALLSKPLVPTSAEHTQTQEYPRPFVDGSNSQSGSELQASPQGQFGEKALTSTNRFIPLANDDPGMQHEMGLDPSMRRRREEWAERGAAKIVKDVVDPATGELTKHVVKMGIKDFKFGEQLGDGSYSSVVLATARDSGKKYAVKVLSKEYLIRQKKVKYVTVEKLALQKLNGTKGIFKLFFTFQDEASLYFLLEYAPHGDFLGLIKKYGSLNETCARYYASQIIDAVDSLHNIGIIHRDIKPENILLDKNMKVKLTDFGTAKILPEEPSNTADGKPYFDLYAKSKSFVGTAEYVSPELLNDNYTDSRCDIWAFGCILYQMLAGKPPFKAANEYLTFQKVMKIQYAFTAGFPQIVKDLVKKLLVRDPNDRLTIKQIKAHLFFHEVNFEDGSVWDDNPPEIQPYKINAEAMKPLQKVSESDTTVKMANLQLAGNGHADTPLQAPAATSQEHSVISMTAATAAFNKDYTSQPKLGSKSSTSVRSASNNTDREVIQKKVSKNRASVSSPSISTTSRGKDNRSRSSDAFWSRYLQNMDERVLLMKEVALSTRNLEDSPVGLENVALDYKNPLDIEPPTDSAGKFYKKMFLITNLGRALVFVKRRSLSMWEEQEFELQFELELNDVEKIRFISDQVLEIDGSRTIFIGCKERAVLMKLWKLIHNGMTAKPKVVSPKSDHKMFDKFILQKRQNTKKKNQAPPVPQSNRLINGLPDRCILKTPEEGALHTKRPTSLQTRSSSNYSKLLARSTQMRKNMTRTDEK 7FFD32E8BE8BC367 7FFD32E8BE8BC367 lsp1_yeast Sphingolipid long chain base-responsive protein LSP1 LSP1 YPL004C protein protein yeast Saccharomyces cerevisiae Baker's yeast MHRTYSLRNQRAPTAAELQAPPPPPSSTKSKFFGKASIASSFRKNAAGNFGPELARKLSQLVKTEKGVLRAMEVVASERREAAKQLSLWGADNDDDVSDVTDKLGVLIYELGELQDQFIDKYDQYRVTLKSIRNIEASVQPSRDRKEKITDEIAHLKYKDPQSTKIPVLEQELVRAEAESLVAEAQLSNITREKLKAAYSYMFDSLRELSEKFALIAGYGKALLELLDDSPVTPGEARPAYDGYEASRQIIMDAESALESWTLDMAAVKPTLSFHQTVDDVYEDEDGEEEEEPEIQNGDIPGQVVEEEEVEWTTEVPVDDEAHEADHHVSQNGHTSGSENI 6FCEE022ECC9778C 6FCEE022ECC9778C mrp8_yeast Uncharacterized protein MRP8 MRP8 YKL3 YKL142W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNEIELLQKQVSELQDLVKKQSLIISKTGERVLELQLDKQKHDVTDFDSKFSKSISKKSGSATQFDATDFATNEDLVELVKELQGELNFIEERSIRRLVNSLKKDDDDVIAPLPNADGDIPAISDGVFPKSLKEFKDIPDLKLVRLAKFYERLPPTLKEQEDFENFLEGKVEAFHINETTDEEISKELEKFSKDELDDAFNDVARYLGLSLRRGTEIW CCAD54B7581A5ABE CCAD54B7581A5ABE yg3a_yeast Uncharacterized protein YGR130C YGR130C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLFNINRQEDDPFTQLINQSSANTQNQQAHQQESPYQFLQKVVSNEPKGKEEWVSPFRQDALANRQNNRAYGEDAKNRKFPTVSATSAYSKQQPKDLGYKNIPKNAKRAKDIRFPTYLTQNEERQYQLLTELELKEKHLKYLKKCQKITDLTKDEKDDTDTTTSSSTSTSSSSSSSSSSSSSSSSDEGDVTSTTTSEATEATADTATTTTTTTSTSTTSTSTTNAVENSADEATSVEEEHEDKVSESTSIGKGTADSAQINVAEPISSENGVLEPRTTDQSGGSKSGVVPTDEQKEEKSDVKKVNPPSGEEKKEVEAEGDAEEETEQSSAEESAERTSTPETSEPESEEDESPIDPSKAPKVPFQEPSRKERTGIFALWKSPTSSSTQKSKTAAPSNPVATPENPELIVKTKEHGYLSKAVYDKINYDEKIHQAWLADLRAKEKDKYDAKNKEYKEKLQDLQNQIDEIENSMKAMREETSEKIEVSKNRLVKKIIDVNAEHNNKKLMILKDTENMKNQKLQEKNEVLDKQTNVKSEIDDLNNEKTNVQKEFNDWTTNLSNLSQQLDAQIFKINQINLKQGKVQNEIDNLEKKKEDLVTQTEENKKLHEKNVQVLESVENKEYLPQINDIDNQISSLLNEVTIIKQENANEKTQLSAITKRLEDERRAHEEQLKLEAEERKRKEENLLEKQRQELEEQAHQAQLDHEQQITQVKQTYNDQLTELQDKLATEEKELEAVKRERTRLQAEKAIEEQTRQKNADEALKQEILSRQHKQAEGIHAAENHKIPNDRSQKNTSVLPKDDSLYEYHTEEDVMYA D482327C9274C026 D482327C9274C026 pil1_yeast Sphingolipid long chain base-responsive protein PIL1 YGR086C PIL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MHRTYSLRNSRAPTASQLQNPPPPPSTTKGRFFGKGGLAYSFRRSAAGAFGPELSRKLSQLVKIEKNVLRSMELTANERRDAAKQLSIWGLENDDDVSDITDKLGVLIYEVSELDDQFIDRYDQYRLTLKSIRDIEGSVQPSRDRKDKITDKIAYLKYKDPQSPKIEVLEQELVRAEAESLVAEAQLSNITRSKLRAAFNYQFDSIIEHSEKIALIAGYGKALLELLDDSPVTPGETRPAYDGYEASKQIIIDAESALNEWTLDSAQVKPTLSFKQDYEDFEPEEGEEEEEEDGQGRWSEDEQEDGQIEEPEQEEEGAVEEHEQVGHQQSESLPQQTTA C86AE5ABB508EA03 C86AE5ABB508EA03 eis1_yeast Eisosome protein 1 YM9973.04C YMR031C EIS1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLISAVEDRDIHNIGKTSGGGSRTSSITSSKKSLKHGSKSLRKPKVYQTTGEPLSREALYKAKLKYGVYQSPAQSYSIGVSDAHAASDKAANLAHDNQTTVEAYKRMFIDPNATKAASKMGPKVVRNNSITSATSKTSKESQTKRKSKESPGAAASKAYSMTMETTSLSSQTNSRSYSITSASSVLSGASGSFNSTVNPKPKTLNLEKVLVGAEKKAESRIKERWEPEKTNFQYGVKTDEHGNLNQFSFSNEMMNNIMAKVDAPKAQDLQKVKKVSAEKEAKSMKFALGAANAVKDMHPGEDIDKSIALKAQKRETYLSQLTSQQVLTLARANVDRQLDIIEKSDMHRKLFTNMEYNKAAVAVAQSNHQKKTEFHNKINMGGGLFLSPEDITKIASGLISPVLGEVSERAEAQRAMDEEIAERTEAYNKSSNEWETMERSIISNDAKVLTTTANRHQTEKKTSQEKIKASFDALVARMDTKVAERETLLEDTKSKEIEFKKQMQQELKDEKARLDQDLEEWGKKCEQDITEARKEQEELLKPYHDDLANAEAEHKTLVEERDEINAEISRLQDAIVDHKRKISGYGNDLDAQKNRNIREDDKLLELGQTKESLESHLNDDVIILANKAKEQAELSTKEARLKQLEVDSLINERKSELNATEIELKKEKLNLLEAMKDVASARGDDKIDEEKVKKLIGMTSEEYLTQNKSVEKNVEDLPTQLEKIEEGDELKKEEIVGAETKNSGGDGVPVSTAAKEATETSSAVQTKEPEEKISIGNKSSGKEDANDCKSAEHSKEISVSQKAGNNKSLGVSPDSLEHTFSGFSQGSSIEDDQDAISNQEKK 0068D65A229B941D 0068D65A229B941D seg2_yeast Eisosome protein SEG2 SEG2 Stability of eisosomes guaranteed protein 2 YKL459 YKL105C protein protein yeast Saccharomyces cerevisiae Baker's yeast MIQESSPDALAAAAAIGNALSYNGRTVDKSKIPQYNQSFTSRTTSIAGINRYTMLSNSRTNSRMLLMNGNVRQYSKRTSSLPNQGHKNTSNNSAGRRQHRAHEDAETTFREFGGKQSSKVLNISSSTGQNSKSRTTSLGNSGSTIRTIKKYIPGPRGLMAVEVPVEVEPPRYSLSNRSNQRGGRAYSLPTRNNKTSLMHRNKTTKKAGSQEKKSESGGKSKNDYHGKVLSKMHTTSLKQRHNNVPLIPTTMNEETEQELQEDLHDPLEFKPMIISDDENSFIEPSVLDRSIPKKDKSGLSGREKKEEIETLLKEVHSLEEKISNIEIAKLNEEEREQSLILELRKVKLDEERRMELLKRELNIVKENADLEAQELKLIESKRKQHFHKGQEVASEVKSITIRQPTLSEPKPAYVPPEDVEKEPSTLSNQTQNIENAENIDSVDAEGNLVDPILLGSLNNSNFHMNSDNEVRCIADSNSLTGSELSDYNYIEGSATDLRATAKTSVESEIGGNQVGLKIPQDDDSEKQEERTKGKKSGLVDTNCFLVQKEDQEEALSDNEPESSEKFPSTSGIENVKLEDETGSVMDKNNGPNNDKDDDDDKDNDNDDDDDKDDDVNDDDKDENVDDDENVDDDDDDDDDDDDEYHDSYDVIMRDPVQIEQDISDVPSLKHPSEYSTETEDNKKKEQNSENAEVSQSGTNMAKYLRGANPYLTNTSSDTFSLDSENVNSKSSTDTTRVAPDLLKSSLQPQLRSDLKQSAVPSSTSSSIYSIETSPNIDSSTGKTASNTKTNSHGPPTSISKQKYDQSSSHQIPVMSPKRLDDKRKITNRSCLRTLRGSSNEATLSHNIVYPASDSSSSPPYHSKKPSNPPSSGNLASHEASKCFPKAPQASTTSRRLPDHVPLYIDKNNSALYPKEPPARKSSFEKERPAKDNLGFRSMSLREPLITKNATATAAENLDVEEKKEKGGHVSRKSWTFGLPSPLKRRTSHSTHTTNETEIVNPMTNFKNKTNENDMPILANKKSCNNDDSSPYTASSMNTNDVSEAGTEGHRFSLFGNKSQLSNRNISGGTATLESSNPDLPTALPLSVPVTIIDKNGEIHKLHNDDAAIKDKSHDRHGHSKFGRKLKKIFGRK 013F55C2CCDD0F9D 013F55C2CCDD0F9D seg1_yeast Eisosome protein SEG1 SEG1 Stability of eisosomes guaranteed protein 1 YM9582.10 YMR086W protein protein yeast Saccharomyces cerevisiae Baker's yeast MFRRRTTAPEMEQADPTAVAAAASIGKLLMKKGNQSDNEQRPTYRSASMTNLRKPSAPKRMSSISSISSESRRSDGKGRPGKINSLTQRSSMGKGDSLNSPLTKEPQHKTRSHNRTSSLPNQRGQQSRNSSGLQRQKSKTHQRISYDEAQRTFKDFGGPQARGILTGQHRTENPSGSIPLRTTRKYIPGPNGLVAIEVPVEKPSNANTSKLLRRSNSAHSALNARNGSLLRKKVSQESLHSQPKKTSSLGNTSSTQAKKGQKAVQERNLAKKHPINSNVPLIETQVREETDQELKLDNSNSSESETVVNSENNLEKPSSLNIEKDDLSKLIHENIELESFIEEKGKEKPLNSDQEDVFISEKTVKGDVQWPKNNRQASTLEKTFNYDNEEKREGNKPVAEHPLPEAEVDDKSVEQLNNISSSGSYSAQGSVENSKPENGNKNLENDTTSSPTQDLDEKSRSIEEKDTLEGTESSKKVEKPDNCCEKISGANTSSKKGNVGDTKDEFFDTVEESDQKTSKSNSSKNTDHSNQTEPTPSLAQYLRTSNTYLSRKNQSKQAEQEKFHKPEAPMVPVTKVVTPIKSALKKSSGSSNHDSSMYSDNSPANGAYLSLTTAENTRLNAQMTMSDSVSRRASLKRSSIKRPQSVGQFRSIRSNSPSPPEKINNKRHSAIPLGTPEKGKPKRNSVMASLSKNSQQIQEPASVYESNGPNKPKNQINKNIKRGSQIAQNNKPSTKDMNSILYPKEPPPRKSSFEKTRSNESHLGFKKLSLRNGNFEEALSESYNGQASQNSTNVNRTDTAQEFFKYLGHSSRFADSDSEDESQFFNQGPSKYNTETEGNKTSGNKNSNGGNGAFSLFKSKSKQKENNVVSPGVSSPNHTTTDPAITSKKVDKKFSGLSLRAASEAEPAKNSNPSMTNRLRFSSNPENGESRLPQAQEVSVTKEKKGSFGKKLKKIFGRKK 05A4FA27129DB09B 05A4FA27129DB09B pil1-lsp1-22 2 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 11 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association lsp1-pil1-22 3 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ymr031c-pil1-1 1 9 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association