IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Traffic (Copenhagen, Denmark) 2010 imex curation Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Accepted 2017-FEB-28 AT 14:35 GMT AT 14:35 GMT by MGT Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation Range cannot finish at 1826 as longer than proteins so amended to C-terminus. Tag is c-terminal - see Table 1 "MATahis4 ura3 leu2 lys2 bar1-1 DOP1::3-HA-kan" Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Traffic (Copenhagen, Denmark) 2010 imex curation Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Accepted 2017-FEB-28 AT 14:35 GMT AT 14:35 GMT by MGT Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation Range cannot finish at 1826 as longer than proteins so amended to C-terminus. Tag is c-terminal - see Table 1 "MATahis4 ura3 leu2 lys2 bar1-1 DOP1::3-HA-kan" Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Traffic (Copenhagen, Denmark) 2010 imex curation Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Barbosa S., Pratte D., Schwarz H., Pipkorn R., Singer-Krüger B. birgit.singer-krueger@tuebingen.mpg.de Accepted 2017-FEB-28 AT 14:35 GMT AT 14:35 GMT by MGT Traffic (Copenhagen, Denmark) 2010 Only protein-protein interactions imex curation imex curation Range cannot finish at 1826 as longer than proteins so amended to C-terminus. Tag is c-terminal - see Table 1 "MATahis4 ura3 leu2 lys2 bar1-1 DOP1::3-HA-kan" mon2_yeast Protein MON2 YNL297C N0453 MON2 YSL2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAMNTGGFDSMQRQLEAELRSLSSESKRRNSTIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIPRSRLSEILDAFIEATHLAMEIQLKVLQVVPIFFKTYGKFIYGPLCKKLLLCCSNLLHVPNKAPVVVGTASATLQQLIDEIFDRLSIESVVDDKQYEVLISNSESIKVNVYRYDANKLFDNICSLNEISSNGAVSDEEMLLDIGDIPIDYGLEILESILKNSQKNLLECQDLQYLLRVKAIPLLLRCISSSRHFSTAVRSCRCLKLLIRKEYLSILELELEVILSLLIHGISVESNLSGWQRVLSLELFKDLSQDPEIVNTLYMDYDNYPDKKHVFKYLLKECIVLLNSPEYITFLAPSKVVEKMDSPLITTENSTVKTKFMHLLDKSNAPSINITYIISLILTICNHLCEGLNKSALESSPLEKKIEDKEREEGTGNDSTVVKVYSGLFSGLFELNKLFLYSTSLETSIFHLVVRAFQKLAHSAGVLSLKDKLRACMKLFSILITNNVTSSNQYSFNDTSKSAKNQHTRNISTSSVTTSPVESTKNPSRSIADSAQNKEMKRRLHPRNISSRQVSLLRALISLSISLGPIFDSESWRYTFLTWQWITYYIYGPSADFKESFYSEDIPPPPILTKSDVTSIESSLTKFFESTSSYSCSTFHLVLTRLILDSKNTLTLEQTNLNLNNDIGYHPLDAKDEIIPCIYNKAFFVNKIGELATYNCKKFLFGKNGKELWSLISTYMIKLISNREMDNDSLRLYTVRVFTDIIKKATNEVGNSDEQDNKVKQFGTLENLVIDSLMATINSIKQLDIGKQEIYNGTINVESDILFQLLLTLKEILNEFGELLMNSWTNIFNIINSPFEWTVEDTDFSVNEDIDDSSLFEGIVQKHKNMIQVSYDVFKLISDDFLQSLPMSVIKFVIDTLVNFVSQKRNLNISFSSISQFWLVGDYLRVRFNPETLNLSDEKRRSLSEKINNQKLIEIITSSSSHDWELYNGLWIYLLKNLINCTNDDRVEVKNGAVQTFFRIIDSHSVCFPPWDLIFLEVIEPLLTKEWSTEELENETDFINVTLQGLIKLYPEHFKDFKNNTTCAKEWSMLLDFLKRLLSSTSNNTKNAVILNYQTLLKEIITIEDVPSDILKKCCEIFTDYNITYSDLSTNASSKTEYDCIYELITGFPPLYQLISKYDAMTDEFVEKVLLLFNSAIKYPLLPEFVQDKTKPSSMQKAILSGLDIFMTNDSKDTEILILLQLSTISILAFDTREKITKKLGPKLPKASLNRLPTFEAISYMSCSNLRNRIAKIDQFGISTLKAKHILRILKNLAEIIKRKSLITGSESDEIPIWVLASNCFCDLSNKIFKSLQEDAENPLKDNFCDLFINVIVVTLQRINPELDNLTEIDDLNEYSKYREILLENRIIDLFNERQLDTFIYAVWDCSFLYEFDELENALMQDCGTFSELSQKLSSFDFSCIFGSTTNPRFQTKYKCSLECLQDLVNFMLNTNEKLRKLTAPYLSARIALALRRYISDEYLIGRAPIPKLRKTELATLLNGLCVILRGVLDQNSTLGNKQIGVENLQTLSPLILRTIPVSHKMDGLQDKVLELSLGFTKLD F0B3D8E6B0D994D6 atc7_yeast Probable phospholipid-transporting ATPase NEO1 NEO1 YIL048W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPNPPSFKSHKQNLFNSNNNQHANSVDSFDLHLDDSFDAALDSLQINNNPEPLSKHNTVGDRESFEMRTVDDLDNFSNHSSDSHRKSSNTDTHPLMYDNRLSQDDNFKFTNIASSPPSSSNNIFSKALSYLKVSNTKNWSKFGSPIELSDQHIEREIHPDTTPVYDRNRYVSNELSNAKYNAVTFVPTLLYEQFKFFYNLYFLVVALSQAVPALRIGYLSSYIVPLAFVLTVTMAKEAIDDIQRRRRDRESNNELYHVITRNRSIPSKDLKVGDLIKVHKGDRIPADLVLLQSSEPSGESFIKTDQLDGETDWKLRVACPLTQNLSENDLINRISITASAPEKSIHKFLGKVTYKDSTSNPLSVDNTLWANTVLASSGFCIACVVYTGRDTRQAMNTTTAKVKTGLLELEINSISKILCACVFALSILLVAFAGFHNDDWYIDILRYLILFSTIIPVSLRVNLDLAKSVYAHQIEHDKTIPETIVRTSTIPEDLGRIEYLLSDKTGTLTQNDMQLKKIHLGTVSYTSETLDIVSDYVQSLVSSKNDSLNNSKVALSTTRKDMSFRVRDMILTLAICHNVTPTFEDDELTYQAASPDEIAIVKFTESVGLSLFKRDRHSISLLHEHSGKTLNYEILQVFPFNSDSKRMGIIVRDEQLDEYWFMQKGADTVMSKIVESNDWLEEETGNMAREGLRTLVIGRKKLNKKIYEQFQKEYNDASLSMLNRDQQMSQVITKYLEHDLELLGLTGVEDKLQKDVKSSIELLRNAGIKIWMLTGDKVETARCVSISAKLISRGQYVHTITKVTRPEGAFNQLEYLKINRNACLLIDGESLGMFLKHYEQEFFDVVVHLPTVIACRCTPQQKADVALVIRKMTGKRVCCIGDGGNDVSMIQCADVGVGIVGKEGKQASLAADFSITQFCHLTELLLWHGRNSYKRSAKLAQFVMHRGLIIAICQAVYSICSLFEPIALYQGWLMVGYATCYTMAPVFSLTLDHDIEESLTKIYPELYKELTEGKSLSYKTFFVWVLLSLFQGSVIQLFSQAFTSLLDTDFTRMVAISFTALVVNELIMVALEIYTWNKTMLVTEIATLLFYIVSVPFLGDYFDLGYMTTVNYYAGLLVILLISIFPVWTAKAIYRRLHPPSYAKVQEFATP DC7225CC9577DBE6 dop1_yeast Protein dopey DOP1 YD9302.17C YDR141C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLPLKPLTIDSNNKQLDSKQKKFRANVERALERFDSVTEWADYIASLGTLLKALQSWSPKFQNVRYYVPSPYQVSRRLTSSLSPALPAGVHQKTLEVYTYIFEHIGLETLATECNIWIPGILPLMTYASMSVRSHLIELYDNYILLLPQTTLRLLIRPLISSLLPGIDDESNDFLPLTLKLIETLQENLDDDSLFWQTLFLVMTANKGRRLGGLTWLTRKFPSLNAVPHLVNKIKMEAEENPSETETNDSHLDRKKRKEEAFKVLLPAAKDLVTPEPGLLIRCLVGCLEDENDILIKRSVLDLLLQRLRLDSPVLNVLITSEDKKLLIMSCCRTTLSKDMSLNRRIWNWLLGPTAGGMLNNNGGNSMEYTTSVKSANEESNVYFTKYGLSALLEGLSDLLSEEESVLTAFRISMAVMDRWEIGSLVIPELFIPLLYSSEKFKQNEQIMKTARTFFDNTETNIIWGKLFQELEDIKNLKILDFVLTNFNIGNDEEIIVRHLPLILLTLLALPSNDKDFDNIYKLQKFSLYNKLLNYIPERALLPLSHSKLKHDDEVSCEELLAKIRGFYTNVSNPSSILEKENIAERLPPFTTEDLTFLIADLIQKKLLSSLWDLENINESSKLFIAIFEKIPESEELKGRSHISWSDKKITQSIFEAIPRLCESNNDAKSEEIVGIVEIFGNYLYSRMEFIESMKLLKVVMMAVWKSLKDPRHQILGVKNLKTLNRFIPSKFIESALVYTFVEEEDISERLSVLDLLWTQLDSDSNLIRRPLELILGELFDDQNPFYLTVSKWILSILNSGSASRLFYILTDNILKVNRLEKERLDERDDLDMLTYEFQMLAYVLKTNNGRTRKVFSTELTSIKSSTIWKNEDVSTYKSLLLVTLMRFLNIKSNTHAKSIRSALILLDILLDGTEQNFKDIVIFLLQMSSKYIAEEGIEPELIAVSLLDIVSKVLRLSHDNGIKLDIFDDNAAHLKYIDFLVTSVSNMKSPLIVTAYVKLLSESIVYFENSIFRMILPLSASLVQCVQRLFLLEKREGGYYQPIALLLGGLEELLEISHGYLVTEEREGYFSGSNLKGDFIQSVVSNVFSSDSSNEESKIQGERDVILQSFRQVISCCLDIWYWAHNISCKSNDDSSLDATNHNSYKFKFRSKKLLETLFLLEPLELLENLISIRSDNTTVTLVHVLDGNKPAITIPHLLYGVIIRYNRTASVKFSNRDGSRSSTTKLTKGEPSMLKRLSGESIIAFLFNYVDSVENSAMEEFYGDFLLFFREVATNYNLYSDVSLSILKLVALISGKVSKTQFGEQKRVRREISDVFFKYLPNAFINFTNLYRGHPDSFKDLEFVVWRVQYIVNDQIGGDKFNTTLATIVNQCLTPYIKPKSEKTIPGYVLELAAVVSHLGSKVKSWRLLIAELFQNDKKLSVIGSDQTWEKIIYEWSIYPENKSKILNDLLLEIGSKRSSVTPTLITFNLGSDSEVEYKCQNLLKISYLLMVSPNDAYLLHFSSLISCIFHYLVSKDIKLKGSCWILLRVLLLRFSESHFNDYWSMISYCLQTNLQEFYESLQIQSEVDPQTILQVCKTLDLLLLLNMEGFTSTNEWIFVIDTINCVYKTNSFVALVDEIAEFKDYEITKTDDLELPTTLKDGLPLLRGIHKIERHTQLRSFFQNLSYLHYEKVYGLGSVDLYGCGEDLKKDILS 119459CD3785526C 119459CD3785526C dop1-mon2-3 2 4 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2A, 9A dop1-neo1-1 3 5 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2B-E DOP1 physically interacts with NEO1 in YSl2-/- cells. dop1-neo1-2 2 5 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P40527:p.[Ile284Thr;Ile449Met;Glu605Gly;Leu680Pro;Tyr714His] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I T certain certain sequence position certain certain certain sequence position certain I M certain certain sequence position certain certain certain sequence position certain E G certain certain sequence position certain certain certain sequence position certain L P certain certain sequence position certain certain certain sequence position certain Y H Wed May 02 19:08:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile284thr,ile449met,glu605gly,leu680pro,tyr714his' Tue Jun 30 19:32:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile284Thr;Ile449Met;Glu605Gly;Leu680Pro;Tyr714His]' P40527:p.[Phe301Ser;Asp305Gly;Val373Ala;Ser539_Leu540delinsProPro;Asn631Ser;Gln655Arg;Val748Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain SL PP certain certain sequence position certain certain certain sequence position certain N S certain certain sequence position certain certain certain sequence position certain Q R certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain F S certain certain sequence position certain certain certain sequence position certain D G Mon Feb 27 13:47:07 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'phe301ser,asp305gly,ser539pro,leu540pro,asn613ser,gln655arg,val748ala' Wed May 02 14:25:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe301ser,asp305gly,val373ala,ser_leu539-540pro_pro,asn631ser,gln655arg,val748ala' Tue Jun 30 20:16:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe301Ser;Asp305Gly;Val373Ala;Ser539_Leu540delinsProPro;Asn631Ser;Gln655Arg;Val748Ala]' 6 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 3D DOP1 physically interacts with NEO1 in MON2-/- cells. dop1-dop1-1 2 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 9c, 9D dop1-dop1-2 1 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 37 34 direct interaction direct interaction Fig. 9 E dop1-neo1-3 2 5 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig.9B NEO1 physically interacts with all regions of DOP1