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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</fullName>
        </names>
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          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="20439702" refType="primary-reference" refTypeAc="MI:0358"/>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</attribute>
            <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Yamamoto D.,  Taoka A.,  Uchihashi T.,  Sasaki H.,  Watanabe H.,  Ando T.,  Fukumori Y.</attribute>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
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        <interactionDetectionMethod>
          <names>
            <shortLabel>pull down</shortLabel>
            <fullName>pull down</fullName>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Yamamoto D., Taoka A., Uchihashi T., Sasaki H., Watanabe H., Ando T., Fukumori Y.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-219763X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</attribute>
        </attributeList>
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      <experimentDescription id="2">
        <names>
          <fullName>Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</fullName>
        </names>
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          <xref>
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            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
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            <attribute name="imex curation" nameAc="MI:0959"/>
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        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-16404" refType="imex-primary" refTypeAc="MI:0662"/>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>molecular sieving</shortLabel>
            <fullName>molecular sieving</fullName>
            <alias type="go synonym" typeAc="MI:0303">Gel Filtration</alias>
            <alias type="go synonym" typeAc="MI:0303">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Gel Filtration</alias>
            <alias type="synonym" typeAc="MI:1041">Sizing column</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0071" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Yamamoto D., Taoka A., Uchihashi T., Sasaki H., Watanabe H., Ando T., Fukumori Y.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-219761X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</attribute>
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      <experimentDescription id="3">
        <names>
          <fullName>Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</fullName>
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            <attribute name="publication year" nameAc="MI:0886">2010</attribute>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>atomic force microsc</shortLabel>
            <fullName>atomic force microscopy</fullName>
            <alias type="go synonym" typeAc="MI:0303">AFM</alias>
            <alias type="synonym" typeAc="MI:1041">AFM</alias>
            <alias type="synonym" typeAc="MI:1041">atomic force microsc</alias>
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          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
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          <attribute name="author-list" nameAc="MI:0636">Yamamoto D., Taoka A., Uchihashi T., Sasaki H., Watanabe H., Ando T., Fukumori Y.</attribute>
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          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2010</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy.</attribute>
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    </experimentList>
    <interactorList>
      <interactor id="4">
        <names>
          <shortLabel>mama_magmg</shortLabel>
          <fullName>Magnetosome protein MamA</fullName>
          <alias type="gene name" typeAc="MI:0301">mamA</alias>
          <alias type="gene name synonym" typeAc="MI:0302">mam22</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0DO20" version="SP_1" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q50224" version="SP_1" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15851915" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_7"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0110143" version="SP_7"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0110146" version="SP_7"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011990" version="SP_7"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR013105" version="SP_7"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR019734" version="SP_7"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-59326N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="188">
          <names>
            <shortLabel>magmg</shortLabel>
            <fullName>Magnetospirillum magnetotacticum</fullName>
            <alias type="synonym" typeAc="MI:1041">Aquaspirillum magnetotacticum</alias>
          </names>
        </organism>
        <sequence>MSSKPSDILDEVTLYAHYGLSVAKKLGMNMVDAFRAAFSVNDDIRQVYYRDKGISHAKAGRYSQAVMLLEQVYDADAFDVDVALHLGIAYVKTGAVDRGTELLERSLADAPDNVKVATVLGLTYVQVQKYDLAVPLLIKVAEANPINFNVRFRLGVALDNLGRFDEAIDSFKIALGLRPNEGKVHRAIAFSYEQMGRHEEALPHFKKANELDEGASV</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q50224 original sequence version: 46</attribute>
          <attribute name="remark-internal">DIP protein Q50224 original sequence version: 53</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="5" imexId="IM-16404-1">
        <names>
          <shortLabel>mam22-1</shortLabel>
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          <primaryRef db="dip" dbAc="MI:0465" id="DIP-187609E" refType="identity" refTypeAc="MI:0356"/>
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          <experimentRef>2</experimentRef>
        </experimentList>
        <participantList>
          <participant id="6">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
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            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
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          </xref>
        </interactionType>
      </interaction>
      <interaction id="7" imexId="IM-16404-2">
        <names>
          <shortLabel>mam22-2</shortLabel>
        </names>
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          <primaryRef db="dip" dbAc="MI:0465" id="DIP-187609E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15851948" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-16404-2" refType="imex-primary" refTypeAc="MI:0662"/>
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        <experimentList>
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        </experimentList>
        <participantList>
          <participant id="8">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
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            </biologicalRole>
            <experimentalRoleList>
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                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
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            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
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          </xref>
        </interactionType>
      </interaction>
      <interaction id="9" imexId="IM-16404-3">
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          <shortLabel>mam22-mam22-1</shortLabel>
        </names>
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          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15851964" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-16404-3" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="10">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="188">
                <names>
                  <shortLabel>magmg</shortLabel>
                  <fullName>Magnetospirillum magnetotacticum</fullName>
                  <alias type="synonym" typeAc="MI:1041">Aquaspirillum magnetotacticum</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="11">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="12">
                <names>
                  <shortLabel>His-tag</shortLabel>
                  <fullName>His-tag</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15851969" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>n-term range</shortLabel>
                        <fullName>n-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">n-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1040" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929795" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>n-term range</shortLabel>
                        <fullName>n-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">n-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1040" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929795" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>