DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Nature 2010 imex curation Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. DIP original experiment: DIP-219505X Nature 2010 IMEx Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Nature 2010 imex curation Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. DIP original experiment: DIP-219507X Nature 2010 IMEx Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. Nature 2010 imex curation Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Scheuermann JC., de Ayala Alonso AG., Oktaba K., Ly-Hartig N., McGinty RK., Fraterman S., Wilm M., Muir TW., Müller J. DIP original experiment: DIP-219506X Nature 2010 IMEx Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. asx_drome Polycomb protein Asx Asx CG8787 Additional sex combs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGPNIAYIDGSNTNSSAVALMEAGNNYIVTTNASPTAAPSPINQQPQSQPTGTQHQHPLLQLHQTGENTPPGNEATATANNCACSLNAMVICQQCGAFCHDDCIGAAKLCVACVIR DIP protein Q9V727 original sequence version: 73 F65D87398D67D321 asxl1_human Polycomb group protein ASXL1 ASXL1 KIAA0978 Additional sex combs-like protein 1 protein protein human Homo sapiens Human MKDKQKKKKERTWAEAARLVLENYSDAPMTPKQILQVIEAEGLKEMRSGTSPLACLNAMLHSNSRGGEGLFYKLPGRISLFTLKKDALQWSRHPATVEGEEPEDTADVESCGSNEASTVSGENDVSLDETSSNASCSTESQSRPLSNPRDSYRASSQANKQKKKTGVMLPRVVLTPLKVNGAHVESASGFSGCHADGESGSPSSSSSGSLALGSAAIRGQAEVTQDPAPLLRGFRKPATGQMKRNRGEEIDFETPGSILVNTNLRALINSRTFHALPSHFQQQLLFLLPEVDRQVGTDGLLRLSSSALNNEFFTHAAQSWRERLADGEFTHEMQVRIRQEMEKEKKVEQWKEKFFEDYYGQKLGLTKEESLQQNVGQEEAEIKSGLCVPGESVRIQRGPATRQRDGHFKKRSRPDLRTRARRNLYKKQESEQAGVAKDAKSVASDVPLYKDGEAKTDPAGLSSPHLPGTSSAAPDLEGPEFPVESVASRIQAEPDNLARASASPDRIPSLPQETVDQEPKDQKRKSFEQAASASFPEKKPRLEDRQSFRNTIESVHTEKPQPTKEEPKVPPIRIQLSRIKPPWVVKGQPTYQICPRIIPTTESSCRGWTGARTLADIKARALQVRGARGHHCHREAATTAIGGGGGPGGGGGGATDEGGGRGSSSGDGGEACGHPEPRGGPSTPGKCTSDLQRTQLLPPYPLNGEHTQAGTAMSRARREDLPSLRKEESCLLQRATVGLTDGLGDASQLPVAPTGDQPCQALPLLSSQTSVAERLVEQPQLHPDVRTECESGTTSWESDDEEQGPTVPADNGPIPSLVGDDTLEKGTGQALDSHPTMKDPVNVTPSSTPESSPTDCLQNRAFDDELGLGGSCPPMRESDTRQENLKTKALVSNSSLHWIPIPSNDEVVKQPKPESREHIPSVEPQVGEEWEKAAPTPPALPGDLTAEEGLDPLDSLTSLWTVPSRGGSDSNGSYCQQVDIEKLKINGDSEALSPHGESTDTASDFEGHLTEDSSEADTREAAVTKGSSVDKDEKPNWNQSAPLSKVNGDMRLVTRTDGMVAPQSWVSRVCAVRQKIPDSLLLASTEYQPRAVCLSMPGSSVEATNPLVMQLLQGSLPLEKVLPPAHDDSMSESPQVPLTKDQSHGSLRMGSLHGLGKNSGMVDGSSPSSLRALKEPLLPDSCETGTGLARIEATQAPGAPQKNCKAVPSFDSLHPVTNPITSSRKLEEMDSKEQFSSFSCEDQKEVRAMSQDSNSNAAPGKSPGDLTTSRTPRFSSPNVISFGPEQTGRALGDQSNVTGQGKKLFGSGNVAATLQRPRPADPMPLPAEIPPVFPSGKLGPSTNSMSGGVQTPREDWAPKPHAFVGSVKNEKTFVGGPLKANAENRKATGHSPLELVGHLEGMPFVMDLPFWKLPREPGKGLSEPLEPSSLPSQLSIKQAFYGKLSKLQLSSTSFNYSSSSPTFPKGLAGSVVQLSHKANFGASHSASLSLQMFTDSSTVESISLQCACSLKAMIMCQGCGAFCHDDCIGPSKLCVLCLVVR DIP protein Q8IXJ9 original sequence version: 71 DIP protein Q8IXJ9 original sequence version: 91 8FCE072F3295AA34 bap1_human Ubiquitin carboxyl-terminal hydrolase BAP1 BAP1 KIAA0272 hucep-6 BRCA1-associated protein 1 Cerebral protein 6 protein protein human Homo sapiens Human MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ DIP protein Q92560 original sequence version: 79 031DA03AE1841D85 calyp_drome Ubiquitin carboxyl-terminal hydrolase calypso calypso BAP1 homolog CG8445 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNAAGGGSGAQAAAVAAGNNSLSHNALLSTASGATTMPMAQLADGWLELESDPGLFTLLLKDFGCHDVQVEEVYDLQKPIESPYGFIFLFRWIEERRARRKIVETTAEIFVKDEEAISSIFFAQQVVPNSCATHALLSVLLNCNENNLQLGDTLSRLKTHTKGMSPENKGLAIGNTPELACAHNSHAMPQARRRLERTGAGVSSCRFTGEAFHFVSFVPINGQLFELDGLKPYPMNHGGWEDSEDWTDKFRRVMAERLGIATGEQDIRFNLMAVVPDRRIAITHKLKMLRTNQAIVSGTLQKLLKADEQGESGNGDSQRPDTPTTLLEPSAFTARDLQSLLKNLDTEIAINEQHLADENDRRHMFKVDASRRTHNYDKFICTFLSMLAHQGVLGELVSQHLLPSKKVSGQGAANRISKQSTTASAGGSTAAGTASTPKTQQQQAAAAKNGKSPSKTPGRRRKGRNKCRKRK DIP protein Q7K5N4 original sequence version: 34 bap1-asxl1-6 2 6 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role prey prey N-terminal domain Region 2-365 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association calypso-asx-1 1 4 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait TAP-tag TAP-tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association calypso-asx-2 3 4 unspecified role unspecified role prey prey N-terminal domain Region 2-337 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association