DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217083X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217080X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217082X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217079X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217084X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by staining molecular weight estimation by staining weight by staining Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217077X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by staining molecular weight estimation by staining weight by staining Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217078X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217085X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. Nature structural & molecular biology 2010 imex curation Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Jaru-Ampornpan P., Shen K., Lam VQ., Ali M., Doniach S., Jia TZ., Shan SO. DIP original experiment: DIP-217081X Nature structural & molecular biology 2010 IMEx ATP-independent reversal of a membrane protein aggregate by a chloroplast SRP subunit. sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE DIP protein O22265 original sequence version: 82 DIP protein O22265 original sequence version: 98 F75ED9C7046A1441 cb5_arath Chlorophyll a-b binding protein CP26, chloroplastic LHCB5 LHCB5 LHCIIc Light-harvesting complex II protein 5 F24G24.140 At4g10340 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASLGVSEMLGTPLNFRAVSRSSAPLASSPSTFKTVALFSKKKPAPAKSKAVSETSDELAKWYGPDRRIFLPDGLLDRSEIPEYLNGEVAGDYGYDPFGLGKKPENFAKYQAFELIHARWAMLGAAGFIIPEALNKYGANCGPEAVWFKTGALLLDGNTLNYFGKNIPINLVLAVVAEVVLLGGAEYYRITNGLDFEDKLHPGGPFDPLGLAKDPEQGALLKVKEIKNGRLAMFAMLGFFIQAYVTGEGPVENLAKHLSDPFGNNLLTVIAGTAERAPTL DIP protein Q9XF89 original sequence version: 78 cab6_arath Chlorophyll a-b binding protein 6, chloroplastic LHCA1 CAB6 LHCI-730 LHCII type III CAB-6 Light-harvesting complex protein Lhca1 F28P10.130 At3g54890 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASNSLMSCGIAAVYPSLLSSSKSKFVSAGVPLPNAGNVGRIRMAAHWMPGEPRPAYLDGSAPGDFGFDPLGLGEVPANLERYKESELIHCRWAMLAVPGILVPEALGYGNWVKAQEWAALPGGQATYLGNPVPWGTLPTILAIEFLAIAFVEHQRSMEKDPEKKKYPGGAFDPLGYSKDPKKLEELKVKEIKNGRLALLAFVGFCVQQSAYPGTGPLENLATHLADPWHNNIGDIVIPFN DIP protein Q01667 original sequence version: 76 2F5EE06B55A979CE sr541_arath Signal recognition particle 54 kDa protein 1 SRP-54A SRP-54 F9L1.25 At1g15310 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVLAELGGRITRAIQQMNNVTIIDEKVLNDFLNEITRALLQSDVSFGLVEKMQTNIKKIVNLDDLAAGHNKRLIIEQAIFKELCRMLDPGKPAFAPKKAKPSVVMFVGLQGAGKTTTCTKYAYYHQKKGYKAALVCADTFRAGAFDQLKQNATKAKIPFYGSYTESDPVKIAVEGVDRFKKEKCDLIIVDTSGRHKQAASLFEEMRQVAEATEPDLVIFVMDSSIGQAAFEQAEAFKETVSVGAVIITKMDGHAKGGGALSAVAATKSPVIFIGTGEHMDEFEVFDVKPFVSRLLGKGDWSGLVDKLQEVVPKDLQNELVENLSQGNFTLRSMYDQFQCSLRIPLNQLFSMLPGISAEMMPKGHGEESRVKMKRYMTMMDSMTNKELDSPNPKIFNESRIMRIARGSGRLVREVMEMLEEYKRIAKTMKGIKIPKNGDMSKVIPPQMLKQMGGMSGLQSLMKQMGSAKDMMGMFGGGGK DIP protein P37106 original sequence version: 78 mint DA4C0634420F486E cb22_pea Chlorophyll a-b binding protein AB80, chloroplastic AB80 LHCII type I CAB-AB80 protein protein pea Pisum sativum (Garden pea) Garden pea MAASSSSSMALSSPTLAGKQLKLNPSSQELGAARFTMRKSATTKKVASSGSPWYGPDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFSKNRELEVIHSRWAMLGALGCVFPELLSRNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWATQVILMGAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLADDPEAFAELKVKELKNGRLAMFSMFGFFVQAIVTGKGPLENLADHLADPVNNNAWSYATNFVPGK DIP protein P07371 original sequence version: 76 D2BD6A9EFC4BAF77 ab80-cao-1 6 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 14 unspecified role unspecified role unspecified role unspecified role Mature protein Region 38-269 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association ab80-cao-2 4 14 unspecified role unspecified role unspecified role unspecified role Mature protein Region 38-269 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ab80-cao-3 9 14 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain Region 199-202 Region 199-202 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P07371:p.Leu201Lys L201K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L K This feature has been corrected as a result of our quality control procedures. The original label was 'L201K'. Wed May 02 17:58:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu201lys' Tue Jun 30 16:29:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu201Lys' ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cao-lhcb5-1 1 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cao-lhca1-1 3 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ab80-1 2 14 unspecified role unspecified role unspecified role unspecified role Mature protein Region 38-269 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ab80-cao-4 7 14 unspecified role unspecified role unspecified role unspecified role Mature protein Region 38-269 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cao-6 5 10 unspecified role unspecified role unspecified role unspecified role Mature protein Region 57-373 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ab80-cao-5 8 14 unspecified role unspecified role unspecified role unspecified role Mature protein Region 38-269 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role O22265:p.Tyr204Ala Y204A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y204A'. Wed May 02 14:42:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr204ala' Tue Jun 30 19:41:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr204Ala' O22265:p.Arg161Ala R161A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R161A'. Wed May 02 15:17:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg161ala' Tue Jun 30 17:43:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg161Ala' Ankyrin 1 domain Region 136-158 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Ankyrin 2 domain Region 159-188 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Ankyrin 3 domain Region 193-222 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Ankyrin 4 domain Region 242-269 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Crhomodomain 1 Region 84-135 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Crhomodomain 2-3 Region 270-373 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction