DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219517X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219512X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219515X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219513X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219516X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219518X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 2010 imex curation Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gao Z., Liu HL., Daxinger L., Pontes O., He X., Qian W., Lin H., Xie M., Lorkovic ZJ., Zhang S., Miki D., Zhan X., Pontier D., Lagrange T., Jin H., Matzke AJ., Matzke M., Pikaard CS., Zhu JK. DIP original experiment: DIP-219514X Nature 2010 IMEx An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. drm2_arath DNA (cytosine-5)-methyltransferase DRM2 DRM2 Protein DOMAINS REARRANGED METHYLASE 2 T15N1.110/T15N1.120 At5g14620/At5g14630 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVIWNNDDDDFLEIDNFQSSPRSSPIHAMQCRVENLAGVAVTTSSLSSPTETTDLVQMGFSDEVFATLFDMGFPVEMISRAIKETGPNVETSVIIDTISKYSSDCEAGSSKSKAIDHFLAMGFDEEKVVKAIQEHGEDNMEAIANALLSCPEAKKLPAAVEEEDGIDWSSSDDDTNYTDMLNSDDEKDPNSNENGSKIRSLVKMGFSELEASLAVERCGENVDIAELTDFLCAAQMAREFSEFYTEHEEQKPRHNIKKRRFESKGEPRSSVDDEPIRLPNPMIGFGVPNEPGLITHRSLPELARGPPFFYYENVALTPKGVWETISRHLFEIPPEFVDSKYFCVAARKRGYIHNLPINNRFQIQPPPKYTIHDAFPLSKRWWPEWDKRTKLNCILTCTGSAQLTNRIRVALEPYNEEPEPPKHVQRYVIDQCKKWNLVWVGKNKAAPLEPDEMESILGFPKNHTRGGGMSRTERFKSLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQIKMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLMEKYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVRARMRGS DIP protein Q9M548 original sequence version: 60 DIP protein Q9M548 original sequence version: 86 10A38FC068636DB7 ago4_arath Protein argonaute 4 At2g27040 AGO4 OCP11 Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 T20P8.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSTNGNGADLESANGANGSGVTEALPPPPPVIPPNVEPVRVKTELAEKKGPVRVPMARKGFGTRGQKIPLLTNHFKVDVANLQGHFFHYSVALFYDDGRPVEQKGVGRKILDKVHQTYHSDLDGKEFAYDGEKTLFTYGALPSNKMDFSVVLEEVSATRANGNGSPNGNESPSDGDRKRLRRPNRSKNFRVEISYAAKIPLQALANAMRGQESENSQEAIRVLDIILRQHAARQGCLLVRQSFFHNDPTNCEPVGGNILGCRGFHSSFRTTQGGMSLNMDVTTTMIIKPGPVVDFLIANQNARDPYSIDWSKAKRTLKNLRVKVSPSGQEFKITGLSDKPCREQTFELKKRNPNENGEFETTEVTVADYFRDTRHIDLQYSADLPCINVGKPKRPTYIPLELCALVPLQRYTKALTTFQRSALVEKSRQKPQERMTVLSKALKVSNYDAEPLLRSCGISISSNFTQVEGRVLPAPKLKMGCGSETFPRNGRWNFNNKEFVEPTKIQRWVVVNFSARCNVRQVVDDLIKIGGSKGIEIASPFQVFEEGNQFRRAPPMIRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCMAPTRQPNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSDVPSIAAVVSSREWPLISKYRASVRTQPSKAEMIESLVKKNGTEDDGIIKELLVDFYTSSNKRKPEHIIIFRDGVSESQFNQVLNIELDQIIEACKLLDANWNPKFLLLVAQKNHHTKFFQPTSPENVPPGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSSHGGITAPGPISVAQLPRLKDNVANSMFFC DIP protein Q9ZVD5 original sequence version: 48 C0323FAE9592B147 nrpb1_arath DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase II largest subunit DNA-directed RNA polymerase II subunit 1 NRPB1 RPB1 RPB205 RPII F4B14.70 At4g35800 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDTRFPFSPAEVSKVRVVQFGILSPDEIRQMSVIHVEHSETTEKGKPKVGGLSDTRLGTIDRKVKCETCMANMAECPGHFGYLELAKPMYHVGFMKTVLSIMRCVCFNCSKILADEEEHKFKQAMKIKNPKNRLKKILDACKNKTKCDGGDDIDDVQSHSTDEPVKKSRGGCGAQQPKLTIEGMKMIAEYKIQRKKNDEPDQLPEPAERKQTLGADRVLSVLKRISDADCQLLGFNPKFARPDWMILEVLPIPPPPVRPSVMMDATSRSEDDLTHQLAMIIRHNENLKRQEKNGAPAHIISEFTQLLQFHIATYFDNELPGQPRATQKSGRPIKSICSRLKAKEGRIRGNLMGKRVDFSARTVITPDPTINIDELGVPWSIALNLTYPETVTPYNIERLKELVDYGPHPPPGKTGAKYIIRDDGQRLDLRYLKKSSDQHLELGYKVERHLQDGDFVLFNRQPSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMVPKCIVSPQANRPVMGIVQDTLLGCRKITKRDTFIEKDVFMNTLMWWEDFDGKVPAPAILKPRPLWTGKQVFNLIIPKQINLLRYSAWHADTETGFITPGDTQVRIERGELLAGTLCKKTLGTSNGSLVHVIWEEVGPDAARKFLGHTQWLVNYWLLQNGFTIGIGDTIADSSTMEKINETISNAKTAVKDLIRQFQGKELDPEPGRTMRDTFENRVNQVLNKARDDAGSSAQKSLAETNNLKAMVTAGSKGSFINISQMTACVGQQNVEGKRIPFGFDGRTLPHFTKDDYGPESRGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTSETGYIQRRLVKAMEDIMVKYDGTVRNSLGDVIQFLYGEDGMDAVWIESQKLDSLKMKKSEFDRTFKYEIDDENWNPTYLSDEHLEDLKGIRELRDVFDAEYSKLETDRFQLGTEIATNGDSTWPLPVNIKRHIWNAQKTFKIDLRKISDMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFFNILLRSTLASKRVLEEYKLSREAFEWVIGEIESRFLQSLVAPGEMIGCVAAQSIGEPATQMTLNTFHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPEASKSKEGAKTVQCALEYTTLRSVTQATEVWYDPDPMSTIIEEDFEFVRSYYEMPDEDVSPDKISPWLLRIELNREMMVDKKLSMADIAEKINLEFDDDLTCIFNDDNAQKLILRIRIMNDEGPKGELQDESAEDDVFLKKIESNMLTEMALRGIPDINKVFIKQVRKSRFDEEGGFKTSEEWMLDTEGVNLLAVMCHEDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCLRGVTENIMLGQLAPIGTGDCELYLNDEMLKNAIELQLPSYMDGLEFGMTPARSPVSGTPYHEGMMSPNYLLSPNMRLSPMSDAQFSPYVGGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARLSPASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGASPDYSPSAGYSPTLPGYSPSSTGQYTPHEGDKKDKTGKKDASKDDKGNP DIP protein P18616 original sequence version: 102 DIP protein P18616 original sequence version: 100 7B8488DBB5180B40 rdm1_arath Protein RDM1 RDM1 Protein RNA-directed DNA methylation 1 MWI23.5 At3g22680 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQSSMTMELRPSGDSGSSDVDAEISDGFSPLDTSHRDVADEGSLLRRAEMYQDYMKQVPIPTNRGSLIPFTSWVGLSISMKQLYGQPLHYLTNVLLQRWDQSRFGTDSEEQRLDSIIHPTKAEATIWLVEEIHRLTPSHLHMALLWRSDPMYHSFIDPIFPEK DIP protein Q9LUJ3 original sequence version: 50 nrpb1-rdm1-1 1 10 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association drm2-rdm1-1 2 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association rdm1-ago4-1 3 9 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association rdm1-drm2-1 4 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association rdm1-rdm1-1 6 11 unspecified role unspecified role prey prey Q9LUJ3:p.[Leu128Arg;Ile132Arg] L128R/I132R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I R certain certain sequence position certain certain certain sequence position certain L R This feature has been corrected as a result of our quality control procedures. The original label was 'L128R/I132R'. Wed May 02 16:51:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu128arg,ile132arg' Tue Jun 30 18:36:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu128Arg;Ile132Arg]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 11 unspecified role unspecified role bait bait Q9LUJ3:p.[Leu128Arg;Ile132Arg] L128R/I132R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I R certain certain sequence position certain certain certain sequence position certain L R This feature has been corrected as a result of our quality control procedures. The original label was 'L128R/I132R'. Wed May 02 17:18:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu128arg,ile132arg' Tue Jun 30 17:22:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu128Arg;Ile132Arg]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction rdm1-nrpb1-1 5 10 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association ago4-rdm1-1 7 9 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 11 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association