DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. DIP original experiment: DIP-219748X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. DIP original experiment: DIP-219746X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. DIP original experiment: DIP-219749X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. DIP original experiment: DIP-219747X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper pull down pull down predetermined predetermined participant predetermined Goley ED., Rammohan A., Znameroski EA., Firat-Karalar EN., Sept D., Welch MD. DIP original experiment: DIP-219745X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. arc1b_human Actin-related protein 2/3 complex subunit 1B Arp2/3 complex 41 kDa subunit p41-ARC ARPC1B ARC41 protein protein human Homo sapiens Human MAYHSFLVEPISCHAWNKDRTQIAICPNNHEVHIYEKSGAKWTKVHELKEHNGQVTGIDWAPESNRIVTCGTDRNAYVWTLKGRTWKPTLVILRINRAARCVRWAPNENKFAVGSGSRVISICYFEQENDWWVCKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRIFSAYIKEVEERPAPTPWGSKMPFGELMFESSSSCGWVHGVCFSASGSRVAWVSHDSTVCLADADKKMAVATLASETLPLLALTFITDNSLVAAGHDCFPVLFTYDAAAGMLSFGGRLDVPKQSSQRGLTARERFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGMDGGMSIWDVKSLESALKDLKIK DIP protein O15143 original sequence version: 110 DIP protein O15143 original sequence version: 150 1939F5B63D40BD27 arpc3_human Actin-related protein 2/3 complex subunit 3 ARPC3 ARC21 Arp2/3 complex 21 kDa subunit protein protein human Homo sapiens Human MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ DIP protein O15145 original sequence version: 91 DIP protein O15145 original sequence version: 136 7149F598B48F0EAC arpc5_human Actin-related protein 2/3 complex subunit 5 ARPC5 ARC16 Arp2/3 complex 16 kDa subunit protein protein human Homo sapiens Human MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV DIP protein O15511 original sequence version: 89 DIP protein O15511 original sequence version: 133 F050B11774EA6E66 arp3_human Actin-related protein 3 ACTR3 ARP3 Actin-like protein 3 protein protein human Homo sapiens Human MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS DIP protein P61158 original sequence version: 117 DIP protein P61158 original sequence version: 84 DIP protein P61158 original sequence version: 127 23E5564198B81C63 arpc2_human Actin-related protein 2/3 complex subunit 2 ARPC2 ARC34 PRO2446 Arp2/3 complex 34 kDa subunit protein protein human Homo sapiens Human MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR DIP protein O15144 original sequence version: 99 DIP protein O15144 original sequence version: 122 DIP protein O15144 original sequence version: 119 DIP protein O15144 original sequence version: 140 3BA57121BE9A05F2 acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 arpc4_human Actin-related protein 2/3 complex subunit 4 ARPC4 ARC20 Arp2/3 complex 20 kDa subunit protein protein human Homo sapiens Human MTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF DIP protein P59998 original sequence version: 77 DIP protein P59998 original sequence version: 123 273CCB230AC703DF arp2_human Actin-related protein 2 ACTR2 ARP2 Actin-like protein 2 protein protein human Homo sapiens Human MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR DIP protein P61160 original sequence version: 78 DIP protein P61160 original sequence version: 121 1BFA6B442ED1A797 actr2-actr3-10 4 8 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 12 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 10 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 13 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association arpc1b-actr2-1 5 8 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper 12 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper 10 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 13 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper association association arpc3-arpc1b-1 1 7 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper proximity proximity close-contact colocalization neighbourhood interaction actr2-actr3-11 2 8 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 12 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 10 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 13 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper association association actin-1 3 11 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction