DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nature structural & molecular biology 2010 imex curation Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. DIP original experiment: DIP-217074X Nature structural & molecular biology 2010 IMEx Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nature structural & molecular biology 2010 imex curation Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly pull down pull down ms participant Identification by mass spectrometry ms participant Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. DIP original experiment: DIP-217076X Nature structural & molecular biology 2010 IMEx Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. Nature structural & molecular biology 2010 imex curation Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Nayak A., Berry B., Tassetto M., Kunitomi M., Acevedo A., Deng C., Krutchinsky A., Gross J., Antoniewski C., Andino R. DIP original experiment: DIP-217075X Nature structural & molecular biology 2010 IMEx Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. ago2_drome Protein argonaute-2 AGO2 CG7439 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGKKDKNKKGGQDSAAAPQPQQQQKQQQQRQQQPQQLQQPQQLQQPQQLQQPQQQQQQQPHQQQQQSSRQQPSTSSGGSRASGFQQGGQQQKSQDAEGWTAQKKQGKQQVQGWTKQGQQGGHQQGRQGQDGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHQQGRQGQEGGYQQRPPGQQQGGHEQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPSGQQQGGHQQGRQGQEGGYQQRPPGQQPNQTQSQGQYQSRGPPQQQQAAPLPLPPQPAGSIKRGTIGKPGQVGINYLDLDLSKMPSVAYHYDVKIMPERPKKFYRQAFEQFRVDQLGGAVLAYDGKASCYSVDKLPLNSQNPEVTVTDRNGRTLRYTIEIKETGDSTIDLKSLTTYMNDRIFDKPMRAMQCVEVVLASPCHNKAIRVGRSFFKMSDPNNRHELDDGYEALVGLYQAFMLGDRPFLNVDISHKSFPISMPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVTIASYFHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVRKRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMVKNGSWRMDGMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKAVNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGTNRFLDLKKEYAKRTIVPEFMKKNPMYFV DIP protein Q9VUQ5 original sequence version: 74 DIP protein Q9VUQ5 original sequence version: 71 DIP protein Q9VUQ5 original sequence version: 73 0DC73AF09CC33F73 poln_crpvc Replicase polyprotein ORF1 protein protein crpvc crpvc CrPV MSFQQTNNNATNNINSLEELAAQELIAAQFEGNLDGFFCTFYVQSKPQLLDLESECYCMDDFDCGCDRIKREEELRKLIFLTSDVYGYNFEEWKGLVWKFVQNYCPEHRYGSTFGNGLLIVSPRFFMDHLDWFQQWKLVSSNDECRAFLRKRTQLLMSGDVESNPGPVQSRPVYACDNDPRAIRLEKALQRRDEKISTLIKKLRQEIKNNRIYTQGFFDDLKGAKGEVGQLNGNLTRICDFLENSLPTLTAQIQTTVLTTTDKYVNLKEDLLKVAILLVLVRLLMVWKKYRAALIVIILFVMHFYGFDKQILDIVLDLKDKILQTTTQAGTETLEEVVYHPWFDTCGKLIFAVLAFFAIKKIPGKQDWDNYISRLDRIPKAIEGSKKIVDYCSEYFNLSVDEVKKVVLGKELKGTQGLYDEIHVWAKEIRHYLDLDERNKITLDTETAAKVEDLYKRGLKYSEEKIPDRDIARFITTMLFPAKSLYEQVLLSPVKGGGPKMRPITVWLTGESGIGKTQMIYPLCIDILREMGIVKPDAYKHQAYARQVETEYWDGYNGQKIVIYDDAFQLKDDKTKPNPEIFEVIRTCNTFPQHLHMAALQDKNMYSQAEVLLYTTNQFQVQLESITFPDAFYNRMKTHAYRVQIKQEKSIWVRNARGEEYNALDVTKLNKDEAIDLSVYEFQKMRFDDESATKWIDDGEPISYDEFARTICKAWKEEKEKTFHQLQWLEAYASRTVAQGGSETSEYYDVWDETYFSNLLSQGFMAGKSLIEMEAEFASDAETFNAYIEYKKNIPKETKWSKWMTILDEQISALSTKIRELKNKAYKFISEHPYLTALGFIGVMISAFAMYSFFERTLTDDTITSEVGSSGDNKTQKISKRVVEVGGSGDVKTTKPAKTAVEVGSSGDSKTMKNKITKVEVGSSGDSKTQKQRNTKVEVGKELEKEAETQGCSDPAAHALVLDVLQKNTYCLYYERMVKGEMKRYRLATATFLRGWVCMMPYHFIETLYARKVAPSTNIYFSQPNCDDVIVVPVSHFIAPNAERVELTTACTRIHYKDETPRDCVLVNLHRRMCHPHRDILKHFVKKSDQGNLRGVFQGTLATFHQSANELCRAYQWLQAIRPLDQEITIYHEDTDMFDYESESYTQRDCYEYNAPTQTGNCGSIVGLYNKRMERKLIGMHIPGNVSECHGYACPLTQEAIMDGLNRLEKLDPVNNITVQCCFEPPSDIKDTMSGETPEGKFCAIGKSNIKVGQAVKTTLLKSCIYGMLSKPITKPAHLTRTRLPNGEIVDPLMKGLKKCGVDTAVLDAEIVESAALDVKQVVLTQYNSMLDVNKYRRFLTYEEATQGTGDDDFMKGIARQTSPGYRYFQMPRKLPGKQDWMGSGEQYDFTSQRAQELRRDVEELIDNCAKGIIKDVVFVDTLKDERRPIEKVDAGKTRVFSAGPQHFVVAFRKYFLPFAAYLMNNRIDNEIAVGTNVYSTDWERIAKRLKKHGNKVIAGDFGNFDGSLVAQFFGQSCGKSFYPWFKTFNDVNTEDGKRNLMICIGLWTHIVHSVHSYGDNVYMWTHSQPSGNPFTVIINCLYNSMIMRIVWILLARKLAPEMQSMKKFRENVSMISYGDDNCLNISDRVVEWFNQITISEQMKEIKHEYTDEGKTGDMVKFPSLSEIHFLKKRFVFSHQLQRTVAPLQKDVIYEMLNWTRNTIDPNEILMMNINTAFREIVYHGKSEYQKLRSGIEDLAMKGILPQQPQILTFKAYLWDATMLADEVYDF 78C5969469EAA716 crpv_1a-ago2-1 2 4 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role bait bait N-terminal region Region 1-148 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association crpv_1a-ago2-2 1 4 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range N-terminal region Region 1-148 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association crpv_1a-ago2-3 3 4 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range N-terminal region Region 1-148 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association