DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Nature 2010 imex curation Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. Only protein-protein interactions chlre Chlamydomonas reinhardtii Chlamydomonas smithii molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. DIP original experiment: DIP-216289X Nature 2010 IMEx Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Nature 2010 imex curation Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. Only protein-protein interactions chlre Chlamydomonas reinhardtii Chlamydomonas smithii pull down pull down western blot western blot Immuno blot Immuno blot Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. DIP original experiment: DIP-216288X Nature 2010 IMEx Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. a8itk6_chlre TEF3 CHLREDRAFT_183448 CHLRE_07g340200v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MQTTVLRSSTRVGCVRPVGLARALPVRPVVFGSRTPMLLRSAKKDDGYISEDEGLGNVAADYCAIDGAGKKAKRSLGEMEQEFLAAMTSWYYEGKPTMSDEEFSLLKEELIWSGSMVAVLSSDEQRFLEASMAYAKGKPIMTDEDYDALKAELRNKSSIVTAQGPRCSIRSKKMYADAEPDYLRMTALNLPGVLFVLGLVFAVDYSTGFGVTKLVELPAPYGPILLWGLLLPSLFTVAYALTQVGFKDNLILKAPCPSCGSENFSYFGDVFTVAGARGQNLVECPNCKADMIFDEYKRVVVVAETSEVKQEKLAAAAAKKAAAAAKKKAKAAA DIP protein A8ITK6 original sequence version: 11 fenr_chlre Ferredoxin--NADP reductase, chloroplastic PETH FNR protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MASLRKPSNHADRACSRRLRVATRVAGRRMCRPVAATKASTAVTTDMSKRTVPTKLEEGEMPLNTYSNKAPFKAKVRSVEKITGPKATGETCHIIIETEGKIPFWEGQSYGVIPPGTKINSKGKEVPTARLYSIASSRYGDDGDGQTASLCVRRAVYVDPETGKEDPAKKGLCSNFLCDATPGTEISMTGPTGKVLLLPADANAPLICVATGTGIAPFRSFWRRCFIENVPSYKFTGLFWLFMGVGNSDAKLYDEELQAIAKAYPGQFRLDYALSREQNNRKGGKMYIQDKVEEYADEIFDLLDNGAHMYFCGLKGMMPGIQDMLERVAKEKGLNYEEWVEGLKHKNQWHVEVY DIP protein P53991 original sequence version: 69 q9zsj4_chlre Chlorophyll a-b binding protein, chloroplastic Lhcb3 LHCBM5 CHLREDRAFT_184775 CHLRE_03g156900v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MMLSRTVVNVQAKLTKKGGAPKKAAPASAQKKTIREKAGWWSNGGNEKLSAFYGPDRGLWLGPLSGTTPAYLTGEFPGDYGWDSAGLSADPETFKRYRELELIHARWAMLGALGCITPELLAKNGTPIVEPVWFKAGAQIFAEGGLDYLGNPGLVHAQSILATLAVQVILMGAIEGYRVNGGPAGEGLDKLHPGGQFFDPLGLAEDPDAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLANLDEHLASPFTSNAFTYAQKFTPQ DIP protein Q9ZSJ4 original sequence version: 44 cb29_chlre Chlorophyll a-b binding protein CP29 Lhcb4 Lhcbm4 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MVFKFPTPPGTQKKAGTTATKPAPKATTKKVATSTGTRSGGVGYRKYQGDALWLPNTTRPEWLDGSLPGDRGFDPLGLSKPSEFVVIGVDENDQNAAKNNKGSVEAIVQATPDEVSSENRLAPYSEVFGLARFRECELIHGRWAMLACLGALVAEATTGVSWVEAGKVELDGASYAGLSLPFSITQLIWIEVILVGGAEFYRNSETNPEKRCYPGGVFDPLKLASEDEERAFRLKTAEIKHARLAMVSFFGYGVQALSTGEGALGSLAKFADGLNNGKGL DIP protein Q93WD2 original sequence version: 79 DIP protein Q93WD2 original sequence version: 51 DIP protein Q93WD2 original sequence version: 50 psaf_chlre Photosystem I reaction center subunit III, chloroplastic Light-harvesting complex I 17 kDa protein PSI-F P21 protein PSAF protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MALTMRNPAVKASSRVAPSSRRALRVACQAQKNETASKVGTALAASALAAAVSLSAPSAAMADIAGLTPCSESKAYAKLEKKELKTLEKRLKQYEADSAPAVALKATMERTKARFANYAKAGLLCGNDGLPHLIADPGLALKYGHAGEVFIPTFGFLYVAGYIGYVGRQYLIAVKGEAKPTDKEIIIDVPLATKLAWQGAGWPLAAVQELQRGTLLEKEENITVSPR DIP protein P12356 original sequence version: 58 DFF1C7B8EC8CB536 cyf_chlre Cytochrome f petA protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MSNQVFTTLRAATLAVILGMAGGLAVSPAQAYPVFAQQNYANPREANGRIVCANCHLAQKAVEIEVPQAVLPDTVFEAVIELPYDKQVKQVLANGKKGDLNVGMVLILPEGFELAPPDRVPAEIKEKVGNLYYQPYSPEQKNILVVGPVPGKKYSEMVVPILSPDPAKNKNVSYLKYPIYFGGNRGRGQVYPDGKKSNNTIYNASAAGKIVAITALSEKKGGFEVSIEKANGEVVVDKIPAGPDLIVKEGQTVQADQPLTNNPNVGGFGQAETEIVLQNPARIQGLLVFFSFVLLTQVLLVLKKKQFEKVQLAEMNF DIP protein P23577 original sequence version: 92 DIP protein P23577 original sequence version: 91 1DC74E50552B7A53 q9fek6_chlre Chlorophyll a-b binding protein, chloroplastic lhcb5 LHCB5 CHLREDRAFT_184397 CHLRE_16g673650v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MQIQALFKKTGASAPAKKGTASTKVVKPSKAGGKATRGWLGGQGGAADLDKWYGPDRKLFLPSGLYDRSEIPEYLNGELAGDYGYDPLGLGKDPETVAKYRENELLHARWAMLAAAGILIPEGLQANGANIKGGTWFETGAEMLNGGTLNYFAVPWGIVSNPLPLFAVIAVEVGLMGAVEFYRRNGTGPAGYSPGIGKFDSSVFDGLDPLYPGGPFDPLGLADDPEVLQELKVKEIKNGRLAMVSVLGFAVQSYVTGEGPYANWTKHVADPFGYNLLTVLGAEERTPTL DIP protein Q9FEK6 original sequence version: 75 DIP protein Q9FEK6 original sequence version: 41 ucria_chlre Cytochrome b6-f complex iron-sulfur subunit, chloroplastic petC Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein Rieske iron-sulfur protein protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MAMLSSRRVAAPAKASAIRRSRVMPVVRAAAASSEVPDMNKRNIMNLILAGGAGLPITTLALGYGAFFVPPSSGGGGGGQAAKDALGNDIKAGEWLKTHLAGDRSLSQGLKGDPTYLIVTADSTIEKYGLNAVCTHLGCVVPWVAAENKFKCPCHGSQYNAEGKVVRGPAPLSLALAHCDVAESGLVTFSTWTETDFRTGLEPWWA DIP protein P49728 original sequence version: 75 7B2C2118E9F75974 cyb6_chlre Cytochrome b6 petB protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MSKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCIGGITFTCFLVQVATGFAMTFYYRPTVAEAFASVQYIMTDVNFGWLIRSIHRWSASMMVLMMVLHVFRVYLTGGFKRPRELTWVTGVIMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPDAIPGVGGFIVELLRGGVGVGQATLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL DIP protein Q00471 original sequence version: 81 E025FB9ED2ED78B4 psaa_chlre Photosystem I P700 chlorophyll a apoprotein A1 psaA ps1a1 PSI-A PsaA protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MTISTPEREAKKVKIAVDRNPVETSFEKWAKPGHFSRTLSKGPNTTTWIWNLHADAHDFDSHTSDLEEISRKVFSAHFGQLGIIFIWLSGMYFHGARFSNYEAWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITSELQLYTTAIGGLVMAAAMFFAGWFHYHKAAPKLEWFQNVESMLNHHLGGLLGLGSLAWAGHQIHVSLPVNKLLDAGVDPKEIPLPHDLLLNRAIMADLYPSFAKGIAPFFTLNWSEYSDFLTFKGGLNPVTGGLWLSDTAHHHVAIAVLFLVAGHMYRTNWGIGHSMKEILEAHRGPFTGEGHVGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYGTQLSLFTHHTWIGGFCIVGAGAHAAIFMVRDYDPTNNYNNLLDRVIRHRDAIISHLNWVCIFLGFHSFGLYIHNDTMSALGRPQDMFSDTAIQLQPVFAQWIQNTHFLAPQLTAPNALAATSLTWGGDLVAVGGKVAMMPISLGTSDFMVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKANLGFRFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVTASGVSHITGGNFAQSANTINGWLRDFLWAQSSQVIQSYGSALSAYGLIFLGAHFVWAFSLMFLFSGRGYWQELIESIVWAHNKLKVAPAIQPRALSITQGRAVGVAHYLLGGIATTWSFFLARIISVG DIP protein P12154 original sequence version: 118 DIP protein P12154 original sequence version: 94 DIP protein P12154 original sequence version: 93 4D8876D1094BDCD3 psaa-lhcb3-1 2 7 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 12 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlre Chlamydomonas reinhardtii Chlamydomonas smithii 3 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 4 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 6 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 9 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 5 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 8 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 10 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii 11 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii association association lhcb3-lhcb4-1 1 3 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 6 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 9 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 5 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 7 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 12 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 11 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii 8 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii physical association physical association