DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216837X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216838X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216840X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216839X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216835X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. Structure (London, England : 1993) 2010 imex curation Lupardus PJ., Birnbaum ME., Garcia KC. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lupardus PJ., Birnbaum ME., Garcia KC. DIP original experiment: DIP-216836X Structure (London, England : 1993) 2010 IMEx Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2. i13r2_human Interleukin-13 receptor subunit alpha-2 IL13RA2 IL13R Interleukin-13-binding protein protein protein human Homo sapiens Human MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGFILILVIFVTGLLLRKPNTYPKMIPEFFCDT DIP protein Q14627 original sequence version: 99 3C6ACB1B5562C887 il13_human Interleukin-13 IL13 NC30 protein protein human Homo sapiens Human MHPLLNPLLLALGLMALLLTTVIALTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN DIP protein P35225 original sequence version: 112 BE2D46CCF913D129 i13r1_human Interleukin-13 receptor subunit alpha-1 Cancer/testis antigen 19 IL13RA1 IL13R IL13RA protein protein human Homo sapiens Human MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNSTLYITMLLIVPVIVAGAIIVLLLYLKRLKIIIFPPIPDPGKIFKEMFGDQNDDTLHWKKYDIYEKQTKEETDSVVLIENLKKASQ DIP protein P78552 original sequence version: 96 5983B3E8F554107B il13-il13ra2-1 1 7 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il13-il13ra2-2 5 7 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il13-il13ra1-4 4 9 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il13ra2-il13-1 2 7 unspecified role unspecified role bait bait Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role prey prey P35225:p.Lys137Ala K137A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K137A'. Wed May 02 18:02:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 19:05:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' P35225:p.Phe140Ala F140A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F140A'. Wed May 02 18:02:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe140ala' Tue Jun 30 15:40:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe140Ala' Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P35225:p.Ile47Ala I47A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I47A'. Wed May 02 14:57:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile47ala' Tue Jun 30 18:00:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile47Ala' P35225:p.Lys138Ala K138A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K138A'. Wed May 02 17:15:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys138ala' Tue Jun 30 18:32:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys138Ala' trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il13-il13ra2-3 6 7 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 29-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role unspecified role unspecified role Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il13ra1-il13-1 3 9 unspecified role unspecified role bait bait trini Trichoplusia ni (Cabbage looper) Cabbage looper 8 unspecified role unspecified role prey prey Region 21-132 Region 21-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P35225:p.Ile47Ala I47A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I47A'. Wed May 02 14:36:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile47ala' Tue Jun 30 17:28:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile47Ala' P35225:p.Lys137Ala K137A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K137A'. Wed May 02 19:27:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys137ala' Tue Jun 30 18:35:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys137Ala' P35225:p.Phe140Ala F140A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F140A'. Wed May 02 18:53:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe140ala' Tue Jun 30 18:58:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe140Ala' P35225:p.Ile123Ala I123A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I123A'. Wed May 02 18:32:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123ala' Tue Jun 30 17:23:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Ala' P35225:p.Glu124Ala E124A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E124A'. Wed May 02 20:19:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu124ala' Tue Jun 30 19:51:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu124Ala' P35225:p.Lys122Ala K122A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K122A'. Wed May 02 19:04:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys122ala' Tue Jun 30 17:44:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys122Ala' P35225:p.Met66Ala M66A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M66A'. Wed May 02 16:32:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met66ala' Tue Jun 30 17:22:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met66Ala' P35225:p.Arg141Ala R141A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R141A'. Wed May 02 19:36:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg141ala' Tue Jun 30 16:34:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg141Ala' trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction