DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216232X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216223X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216221X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216225X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216231X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216233X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216227X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216228X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216229X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216220X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216226X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216222X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216224X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature 2010 imex curation Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216230X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. arf_human Tumor suppressor ARF p14ARF CDKN2A CDKN2 MLM Alternative reading frame Cyclin-dependent kinase inhibitor 2A protein protein human Homo sapiens Human MVRRFLVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRLGQQPLPRRPGHDDGQRPSGGAAAAPRRGAQLRRPRHSHPTRARRCPGGLPGHAGGAAPGRGAAGRARCLGPSARGPG DIP protein Q8N726 original sequence version: 97 DIP protein Q8N726 original sequence version: 113 DIP protein Q8N726 original sequence version: 72 7739A9050C21BC96 myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 p06748-1 Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL DIP protein P06748-1 original sequence version: 180 DIP protein P06748 original sequence version: 138 DIP protein P06748-1 original sequence version: 138 DIP protein P06748 original sequence version: 178 DIP protein P06748 original sequence version: 132 620BC7BA2E4A0054 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tripc_human E3 ubiquitin-protein ligase TRIP12 TRIP12 Thyroid receptor-interacting protein 12 KIAA0045 E3 ubiquitin-protein ligase for Arf ULF HECT-type E3 ubiquitin transferase TRIP12 protein protein human Homo sapiens Human MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS This sequence has been withdrawn from Uniprot. 294A7C063A3332DE 294A7C063A3332DE cdkn2a-ub-2 6 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ulf-cdkn2a-1 10 15 unspecified role unspecified role prey prey human Homo sapiens Human 19 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ulf-cdkn2a-2 4 15 unspecified role unspecified role prey prey human Homo sapiens Human 19 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range This tag was used for western blot detection. human Homo sapiens Human physical association physical association cdkn2a-ulf-1 3 15 unspecified role unspecified role bait bait human Homo sapiens Human 19 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association myc-ulf-1 9 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 19 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-759 Region 1-759 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction ulf-myc-1 14 16 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 19 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range This tag was used for western blot detection. human Homo sapiens Human physical association physical association cdkn2a-ulf-2 12 15 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 19 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-759 Region 1-759 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction npm1-ulf-1 11 17 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 19 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association myc-ulf-2 5 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ULF-binding domain Region 262-439 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 19 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction ulf-myc-2 7 16 unspecified role unspecified role prey prey human Homo sapiens Human 19 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association cdkn2a-ub-3 2 15 unspecified role unspecified role bait bait human Homo sapiens Human 18 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human physical association physical association ulf-myc-3 1 16 unspecified role unspecified role prey prey ULF-binding domain Region 329-439 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 19 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range This tag was used for western blot detection. human Homo sapiens Human physical association physical association ulf-myc-4 13 17 unspecified role unspecified role prey prey human Homo sapiens Human 16 unspecified role unspecified role prey prey human Homo sapiens Human 19 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range This tag was used for western blot detection. human Homo sapiens Human association association myc-ulf-3 8 16 unspecified role unspecified role bait bait human Homo sapiens Human 19 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association