DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Li L., Ye H., Guo H., Yin Y. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li L., Ye H., Guo H., Yin Y. DIP original experiment: DIP-216301X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Li L., Ye H., Guo H., Yin Y. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Li L., Ye H., Guo H., Yin Y. DIP original experiment: DIP-216303X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Li L., Ye H., Guo H., Yin Y. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li L., Ye H., Guo H., Yin Y. DIP original experiment: DIP-216302X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression. bzr2_arath Protein BRASSINAZOLE-RESISTANT 2 BZR2 BES1 BIS1 At1g19350 F18O14_4 Protein BRI1-EMS-SUPPRESSOR 1 Protein BIN2 SUBSTRATE 1 Protein 107 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSDGATSTSAAAAAAAMATRRKPSWRERENNRRRERRRRAVAAKIYTGLRAQGNYNLPKHCDNNEVLKALCSEAGWVVEEDGTTYRKGHKPLPGDMAGSSSRATPYSSHNQSPLSSTFDSPILSYQVSPSSSSFPSPSRVGDPHNISTIFPFLRNGGIPSSLPPLRISNSAPVTPPVSSPTSRNPKPLPTWESFTKQSMSMAAKQSMTSLNYPFYAVSAPASPTHHRQFHAPATIPECDESDSSTVDSGHWISFQKFAQQQPFSASMVPTSPTFNLVKPAPQQLSPNTAAIQEIGQSSEFKFENSQVKPWEGERIHDVAMEDLELTLGNGKAHS DIP protein Q9LN63 original sequence version: 82 DIP protein Q9LN63 original sequence version: 47 DIP protein Q9LN63 original sequence version: 60 DIP protein Q9LN63 original sequence version: 79 DIP protein Q9LN63 original sequence version: 99 7B0AA929FED4748B iws2_arath Protein IWS1 homolog 2 IWS2 At4g19000 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQELDSSNDEWVKELEGENEESKFTGRRLVKKSISVPELVDEVEEDLDDFTEPADDFNDKVGKKRQRKKKDESGLEKTKKNKKQNSEEVQEMWDSITNNTNSQYGDKVVVKPPKKKDEDAEEIKKLFSLRKKKSKCDKTSMEIGMQVEQVMANLEIAVEDDVICNREGKPAINKLMKLPLLNETLSKKPLQGEFLDHGVLNLLKNWLEPLPDGSLPNINIRSAVLMILNDFRIDLDQDSRREQLIKSGLGKVIMFLSKSDEETTPNRRLANDIINKWGRIIYNKSTRYDNMFTQEELDEQRQILLRRQTKTAPKVSGTRARDFNTDIDLYELGTWTGRARAKIPTTMSMDFKIRPPSKVDINQEEEPCSKWQMEKRHKNKQQKNIRKGGMQALKLSVDGRTMLKYL DIP protein O49413 original sequence version: 45 bzr2-iws2-1 2 4 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP cYFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 5 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP nYFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress direct interaction direct interaction bzr2-iws2-2 1 4 unspecified role unspecified role bait bait Region 1-271 Region 1-271 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction bzr2-iws2-3 3 5 unspecified role unspecified role prey prey MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role bait bait Region 140-271 Region 140-271 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction