DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A role for FKBP52 in Tau protein function. A role for FKBP52 in Tau protein function. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. DIP original experiment: DIP-219245X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A role for FKBP52 in Tau protein function. A role for FKBP52 in Tau protein function. A role for FKBP52 in Tau protein function. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. Only protein-protein interactions rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. DIP original experiment: DIP-219243X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A role for FKBP52 in Tau protein function. A role for FKBP52 in Tau protein function. A role for FKBP52 in Tau protein function. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu EE. DIP original experiment: DIP-219244X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A role for FKBP52 in Tau protein function. fkbp4_human Peptidyl-prolyl cis-trans isomerase FKBP4 FK506-binding protein 4 HSP-binding immunophilin Rotamase 52 kDa FK506-binding protein FKBP59 Immunophilin FKBP52 FKBP4 FKBP52 51 kDa FK506-binding protein 59 kDa immunophilin protein protein human Homo sapiens Human MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKYKQALLQYKKIVSWLEYESSFSNEEAQKAQALRLASHLNLAMCHLKLQAFSAAIESCNKALELDSNNEKGLFRRGEAHLAVNDFELARADFQKVLQLYPNNKAAKTQLAVCQQRIRRQLAREKKLYANMFERLAEEENKAKAEASSGDHPTDTEMKEEQKSNTAGSQSQVETEA DIP protein Q02790 original sequence version: 130 DIP protein Q02790 original sequence version: 128 DIP protein Q02790 original sequence version: 144 6A498105418D9435 fkbp4_rat Peptidyl-prolyl cis-trans isomerase FKBP4 Fkbp4 Fkbp52 52 kDa FK506-binding protein 59 kDa immunophilin FK506-binding protein 4 FKBP59 HSP-binding immunophilin Immunophilin FKBP52 Rotamase protein protein rat Rattus norvegicus (Rat) Rat MTAEEMKVAENGAQSAPLPLEGVDISPKQDEGVLKVIKREGTGTETAMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAVATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEDEDGGIIRRIRTRGEGYARPNDGAMVEVALEGYYNDRLFDQRELCFEVGEGESLDLPCGLEEAIQRMEKGEHSIVYLKPSYAFGSVGKERFQIPPHAELRYEVHLKSFEKAKASWEMNSEEKLEQSNIVKERGTVYFKEGKYKQALLQYKKIVSWLEYESSFSGEEMQKVHALRLASHLNLAMCHLKLQAFSAAIESCNKALELDSNNEKGLFRRGEAHLAVNDFDLARADFQKVLQLYPSNKAAKTQLAVCQQRTRRQLAREKKLYANMFERLAEEEHKAKTEVAAGDHPTDAEMKGEPNNVAGNQAQVKTEA DIP protein Q9QVC8 original sequence version: 71 p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636-8 original sequence version: 206 DIP protein P10636-8 original sequence version: 203 DIP protein P10636-8 original sequence version: 166 DIP protein P10636-8 original sequence version: 218 835A8706D847A8CC tau_rat Microtubule-associated protein tau Mapt Mtapt Tau Neurofibrillary tangle protein Paired helical filament-tau protein protein rat Rattus norvegicus (Rat) Rat MAEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P19332 original sequence version: 107 mint Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization 915DCA04EF0B2902 fkbp4-mapt-1 2 7 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat 5 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat physical association physical association fkbp4-mapt-2 3 7 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat 4 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association mapt-fkbp4-1 1 6 unspecified role unspecified role bait bait ecolx Escherichia coli 4 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction